Histochemistry

, Volume 49, Issue 4, pp 327–335 | Cite as

Histochemical findings in the rat gastric mucosa during starvation

  • M. Zaviačič
  • M. Brozman
Article

Summary

The influence of starving on the activity of enzymes of the rat gastric mucosa was investigated by selected histochemical methods. Beside the conventional methods of enzymatic histochemistry the technique of semipermeable membranes was used in the proof of lysosomal enzymes. Dehydrogenases were proved in aqueous and also in gel media with PMS.

During the starvation in the parietal cells a marked increase took place in the activity of acid phosphatase, E-600 resistant esterase, less in β-glucuronidase. High activity of the lysosomal enzymes in macrophages did not change during starvation. Nor did any changes took place in the activity of alkaline phosphatase in the endothelium of the capillaries. The chief cells in the control and starving animals, in contrast to the human gastric mucosa, did not contain any non-specific esterase. Concerning dehydrogenase, parietal cells with a different activity of these enzymes were observed both in starved and control animals.

In the rat gastric mucosa starving induced changes in the activity of the enzymes which mark important organelles of the cells. Thus it is possible to consider the observed histochemical changes as a functional manifestation of morphological damage of cellular structures which are affected during starvation.

Keywords

Gastric Mucosa Acid Phosphatase Parietal Cell Lysosomal Enzyme Chief Cell 

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References

  1. Bednář, B., Miřejovský, P., Stanová, M.: Changes in the hepatocyte during starving. Čs. Pat. 9, 78–86 (1973) (in Czech.)Google Scholar
  2. Burstone, M.S.: Enzyme histochemistry and its application in the study of neoplasms, pp. 464. Berlin-Göttingen-Heidelberg: Springer 1962Google Scholar
  3. Cardell, R.R.: Action of metabolic hormones on the fine structure of rat liver cells. I. Effects of fasting on the ultrastructure of hepatocytes. Amer. J. Anat. 131, 21–54 (1971)Google Scholar
  4. Correia, J.P., Filipe, M.I., Santos, J.C.: Histochemistry of the gastric mucosa. I. In normal mucosa. II. In pathological conditions and in changes induced by histamine. Gut 4, 68–76 (1963)Google Scholar
  5. David, H.: Zur Mitochondriengröße in den Lebern von Hunger-Mäusen (Mikroskopische und submikroskopische Untersuchungen). Acta biol. med. germ. 4, 159–165 (1960)Google Scholar
  6. Davis, B.J., Ornstein, L.: High resolution enzyme localization with a new diazo reagent “Hexazonium Pararosaniline”. J. Histochem. Cytochem. 7, 297–298 (1959)Google Scholar
  7. Ďuriš, I., Zaviačič, M., Hollý, D., Ondrejička, M.: Diphenylamine reaction of gastric juice. Correlation of the reaction with histochemical and histoenzymological findings of gastric mucosa. Gastroent. jap. 5, 204–214 (1970)Google Scholar
  8. Floch, M.H., van Noorden, S., Spiro, H.M.: Histochemical localization of gastric and small bowel mucosal enzymes of man, monkey, and chimpanzee. Gastroenterology 52, 230–238 (1967)Google Scholar
  9. Gasbarrini, G., Faggioli, F., Roda, E.: Aspetti ultramicroscopici degli epiteli nel digiuno protratto. II. Osservationi sul pangreas esocrine del ratto. G. Clin. med. 49, 1302–1310 (1968)Google Scholar
  10. Hayashi, M.: Histochemical demonstration of N-acetyl-beta-glucosaminidase employing Naphthol ASBI N-acetyl-beta-glucosaminide. J. Histochem. Cytochem. 13, 355–360 (1965)Google Scholar
  11. Hayashi, M., Nakajima, Y., Fishman, W.H.: The cytologic demonstration of beta-glucuronidase employing naphthol ASBI glucuronide and hexazonium-para-rosaniline. A preliminary report. J. Histochem. Cytochem. 12, 293–297 (1964)Google Scholar
  12. Helander, H.F.: Ultrastructure of fundus glands of the mouse gastric mucosa. An electron microscopical study in fasted and refed animals with observations on ultrastructure after different fixation and embedding procedures. J. Ultrastruct. Res., Suppl. 4, 1–123 (1962)Google Scholar
  13. Herdson, P.B., Garvin, P.J., Jenings, R.B.: Fine structural changes produced in rat liver by partial starvation. Amer. J. Path. 65, 157–181 (1964)Google Scholar
  14. Holt, S.J., Withers, R.F.J.: Studies in enzyme cytochemistry. V. An appraisal of indigogenic reactions for esterase localization. Proc. roy. Soc. Edinb. B 148, 520–531 (1958)Google Scholar
  15. Kapeller, K., Mikulajová, M., Bučko, A.: Ultrastructure of the acinar cell of the rat pancreas during starving. Bratisl. lek. Listy 55, 70–79 (1971) in SlovakGoogle Scholar
  16. Komáromy, L., Montskó, T., Tigyi, A., Lissak, K.: Light and electron microscopic studies of the acinar cells of the pancreas after fasting and feeding. Acta physiol. Acad. Sci. hung. 31, 209–216 (1967)Google Scholar
  17. Komnick, H.: Zur funktionellen Morphologie der Salzsäureproduktion in der Magenschleimhaut. Histochemischer Chloridnachweis mit Hilfe der Elektronenmikroskopie. Histochemie 3, 354 (1963)Google Scholar
  18. Lojda, Z.: Fixation in histochemistry. Folia morph. Prague 13, 65–83 (1965a)Google Scholar
  19. Lojda, Z.: Remarks on histochemical demonstration of dehydrogenases. II. Intracellular localization. Folia morph. Prague 13, 84–96 (1965b)Google Scholar
  20. Lojda, Z.: Introduction to enzyme histochemistry. Part I, pp. 27. Brno: Czechoslovak Society of Histo- and Cytochemistry 1966 (in Czech.)Google Scholar
  21. Lojda, Z.: Histochemical detection of enzymes. Part II, pp. 21. Brno: Czechoslovak Society of Histo- and Cytochemistry 1968 [in Czech]Google Scholar
  22. Lojda, Z.: Basis on histochemical detection of enzymes, pp. 86. Brno: Czechoslovak Society of Histo- and Cytochemistry 1970 [in Czech]Google Scholar
  23. Lojda, Z.: Aktuelle Probleme der Cytochemie der lysosomalen Hydrolasen. Acta morph. Acad. Sci. hung. 20, 269–293 (1972)Google Scholar
  24. Lojda, Z., Večerek, B., Polichová, H.: Some remarks concerning the histochemical detection of acid phosphatase by azocoupling reactions. Histochemie 3, 428–454 (1964)Google Scholar
  25. Nevalainen, T.J., Janigan, D.T.: Degeneration of mouse pancreatic acinar cells during fasting. Virchows Arch. Abt. B Zellpath. 15, 107–118 (1974)Google Scholar
  26. Paradisi, F., Cavazzuti, F.: Exocrine pancreas and experimental dietary lesions. Gastroenterologia 103, 1–14 (1965)Google Scholar
  27. Pearse, A.G.E.: Histochemistry. Theoretical and applied, 3rd ed., Vol. 2, pp. 1518. Edinburgh and London: Churchill Livingstone 1972Google Scholar
  28. Planteydt, H.T., Willighagen, R.G.J.: Enzyme histochemistry of the human stomach with special reference to intestinal metaplasia. J. Path. Bact. 80, 317–323 (1960)Google Scholar
  29. Ragins, H., Dittbrenner, M.: Intracellular enzymatic histochemistry of the human stomach with special reference to atrophic gastritis. Gut 6, 357–363 (1965)Google Scholar
  30. Scarpelli, D.G., Chiga, M., Haynes, E.: Population changes in rat liver mitochondria after inanition and refeeding: A possible model of mitochondriogenesis. J. Cell Biol. 39, 119 (1968)Google Scholar
  31. Shrader, R.E., Zeman, F.J.: Histochemically demonstrable enzymes in the organs of digestive system of the newborn rat. Progr. Histochem. Cytochem. 3, 125–178 (1972)Google Scholar
  32. Siegel, H.I., Lev, R., Grall, G.B.J.: Enzyme histochemistry of chronic atrophic gastritis. Amer. J. dig. Dis. 13, 974–986 (1968)Google Scholar
  33. Tigyi, A., Montskó, T., Komáromy, L., Lissak, K.: Electron microscopic studies of the pancreatic acinar cell organelles in various functional condition. Acta physiol. Acad. Sci. hung. 32, 19–24 (1967)Google Scholar
  34. Webster, P.D., Singh, M., Tucker, P.C., Black, O.: Effects of fasting and feeding on the pancreas. Gastroenterology 62, 600–605 (1972)Google Scholar
  35. Zaviačič, M.: Influence of fasting on the structure of gastric mucosa. Thesis for CSc. degree Komenský University, Medical School, pp. 178, Bratislava 1974a [in Slovak]Google Scholar
  36. Zaviačič, M.: Influence of starving on the histochemical picture of rat pancreas, pp. 70–76. In: Bučko, A., Kopec, Z., Nutrition and pancreas. Bratislava: VEDA vydavatel'stvo SAV 1974b [in Slovak, Summary in English]Google Scholar
  37. Zaviačič, M., Brozman, M.: Histochemical localisation of enzymes in the normal rat gastric mucosa using the technique of the semipermeable membranes and the other methods. Acta histochem. (Jena) (1976d) in pressGoogle Scholar
  38. Zaviačič, M., Brozman, M., Jakubovský, J.: Influence of fasting and stimulation on the rat gastric endocrine cells. Histochemical and ultrastructural study. Histochemistry (1976a) in pressGoogle Scholar
  39. Zaviačič, M., Brozman, M., Jakubovský, J.: Influence of starving on the rat gastric mucosa. Light and electron microscopical findings. (Submitted to publication, 1976b)Google Scholar
  40. Zaviačič, M., Brozman, M., Jakubovský, J., Ďuriš, I.: Ultrastructure of the human antral G cells during fasting. (Submitted to publication, 1976c)Google Scholar
  41. Zaviačič, M., Brozman, M., Jakubovský, J., Ďuriš, I., Koška, M., Hollý, D.: Histoenzymatic and ultrastructural findings in the human gastric mucosa during fasting. Gastroent. jap. 10, 261–270 (1975)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • M. Zaviačič
    • 1
  • M. Brozman
    • 1
  1. 1.Institute of Pathology, Medical SchoolKomenský University BratislavaBratislavaCzechoslovakia

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