, Volume 56, Issue 3–4, pp 239–244 | Cite as

The localization of calcium by X-ray microanalysis in myopathic muscle fibers

  • Ernö Dux
  • Ida Tóth
  • László Dux
  • Ferenc Joó


An electron histochemical study was undertaken to localize calcium with ammonium oxalate precipitation technique in soleus muscle of rat in normal cases and in myopathy induced experimentally by a prolonged treatment of 2,4-dichlorophenoxyacetate (2,4-D). The calcium content of precipitates was detected by energy-dispersive X-ray microanalysis. In normal cases, the electron dense precipitates containing calcium were mainly found in the vesicles of sarcoplasmic reticulum, whereas in 2,4-D induced myopathy the deposits were shifted near the Z line into the myofibrils. Calcium, because the uptake into sarcoplasmic vesicles was inhibited by 2,4-D, could attach to other binding sites, such as to the troponin-C.A long-lasting binding of calcium might lead to a prolonged activation of the actin-myosin system.


Calcium Oxalate Myopathy Sarcoplasmic Reticulum Calcium Content 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Ernö Dux
    • 1
  • Ida Tóth
    • 1
  • László Dux
    • 2
  • Ferenc Joó
  1. 1.Institute of Biophysics, Biological Research CenterHungarian Academy of SciencesSzegedHungary
  2. 2.Institute of BiochemistryMedical University of SzegedSzegedHungary

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