Summary
Subcutaneous (SC) injections of ceruletide (caerulein diethylammonium hydrate, CER) and the octapeptide of cholecystokinin (CCK-8) reduced the intake of liquid food in male NMRI mice starved for 18 h. The corresponding ED50 values were 2 μg/kg for CER and 24 μg/kg for CCK-8; hence, on a molar basis, CER was 14 times more potent than CCK-8. Naloxone (0.2 and 1 mg/kg, SC) inhibited eating. (d-Ala)2 (MePhe)4-(Met(O)-ol)5-enkephalin (FK 33-824; 0.3 and 1 mg/kg) was only stimulatory. Naloxone enhanced the effect of CER, whereas FK 33-824 antagonized it. It is concluded that concerning the inhibition of food intake, opioid peptides can be antagonists of CCK-like peptides. This is consistent with the current view of the regulation of appetitive behaviour.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brown DR, Holtzman SG (1979) Suppression of deprivationinduced food and water intake in rats and mice by naloxone. Pharmacol Biochem Behav 11:567–573
Crawley JN, Rojas-Ramirez JA, Mendelson WB (1982) The role of central and peripheral cholecystokinin in mediating appetitive behaviors. Peptides 3:535–538
Deschodt-Lanckman M, Bui ND, Noyer M (1981) Degradation of cholecystokinin-like peptides by a crude rat brain synaptosomal fraction: A study by high pressure liquid chromatography. Regul Peptides 2:15–30
Dockray GJ (1982) The physiology of cholecystokinin in brain and gut. Br Med Bull 38:253–258
Extein I, Goodwin FK, Lewy AJ, Schoenfeld RI, Fakhuri LR, Gold MS, Redmond DE (1979) Behavioral and biochemical effects of FK 33-824, a parenterally and orally active enkephalin analogue. In: Usdin E, Bunney WE Jr, Kline NS (eds) Endorphins in mental health. Macmillan Press, London, pp 279–292
Holtzman SG (1974) Behavioral effects of separate and combined administration of naloxone and d-amphetamine. J Pharmacol Exp Ther 189:51–60
Innis RB, Snyder SH (1980) Distinct cholecystokinin receptors in brain and pancreas. Proc Natl Acad Sci USA 77:6917–6921
Itoh S, Katsuura G (1982) Effects of β-endorphin, thyrotropinreleasing hormone and cholecystokinin on body-shaking behavior in rats. Jpn J Physiol 32:667–675
Jalowiec JE, Panksepp J, Zolovick AJ, Najam N, Herman BH (1981) Opioid modulation of ingestive behavior. Pharmacol Biochem Behav 15:477–484
Jones JG, Richter JA (1981) The site of action of naloxone in suppressing food and water intake in rats. Life Sci 28:2055–2064
Jurna I, Zetler G (1981) Antinociceptive effect of centrally administered caerulein and cholecystokinin octapeptide (CCK-8). Eur J Pharmacol 73:323–331
Manara L, Bianchi G, Fiocchi R, Notarnicola A, Peracchia F, Tavani A (1983) Inhibition of gastrointestinal transit, by morphine and FK 33-824 in the rat and comparative narcotic antagonist properties of naloxone and its N-methyl-quaternary analog. Life Sci 31:1271–1274
Margules DL, Moisset B, Lewis MJ, Shibuya H, Pert CB (1978) β-endorphin is associated with overeating in genetically obese mice (ob/ob) and rats (fa/fa). Science 202:988–991
McLaughlin CL, Baile CA (1981) Obese mice and the satiety effects of cholecystokinin, bombesin and pancreatic polypeptide. Physiol Behav 26:433–438
Moran TH, McHugh PR (1982) Cholecystokinin suppresses food intake by inhibiting gastric emptying. Am J Physiol 242:R491-R497
Morley JE (1982) The ascent of cholecystokinin (CCK) from gut to brain. Life Sci 30:479–493
Morley JE, Levine AS (1982) the role of the endogenous opiates as regulators of appetite. Am J Clin Nutr 35:757–761
Morley JE, Levine AS, Kneip J, Grace M (1982a) The effect of vagotomy on the satiety effects of neuropeptides and naloxone. Life Sci 30:1943–1947
Morley JE, Levine AS, Murray SS, Kneip J, Grace M (1982b) Peptidergic regulation of stress-induced eating. Am J Physiol 243:R159-R163
Morley JE, Levine AS, Yim GK, Lowy MT (1983) Opioid modulation of appetite. Neurosci Biobehav Rev 7:227–256
Newmark SR (1981) Caerulein inhibits food intake in the rat and the mouse. Nutr Rep Int 23:151–154
Parrott RF, Batt RAL (1980) The feeding response of obese mice (genotype, ob ob) and their wild-type littermates to cholecystokinin (pancreozymin). Physiol Behav 24:751–754
Roemer D, Buescher HH, Hill RC, Pless J, Bauer W, Cardinaux F, Closse A, Hauser D, Huguenin R (1977) A synthetic enkephalin analogue with prolonged parenteral and oral analgesic activity. Nature 268:547–549
Schally AV, Redding TW, Lucien HW, Meyer J (1967) Enterogastrone inhibits eating by fasted mice. Science 157:210–211
Scheufler E, Zetler G (1981) Interactions of caerulein and morphine on gastrointestinal propulsion in the mouse. Naunyn-Schmiedeberg's Arch Pharmacol 318:66–69
Strohmayer AJ, Smith GP (1981) Cholecystokinin inhibits food intake in genetically obese (C57BL/6J-ob) mice. Peptides 2:39–44
Van Ree JM, Gaffori O, De Wied D (1983) In rats, the behavioral profile of CCK-8 related peptides resembles that of antipsychotic agents. Eur J Pharmacol 93:63–78
Zetler G (1979) Antagonism of cholecystokin-like peptides by opioid peptides, morphine or tetrodotoxin. Eur J Pharmacol 60:67–77
Zetler G (1982a) Cholecystokinin octapeptide, caerulein and caerulein analogues: effects on thermoregulation in the mouse. Neuropharmacology 21:795–801
Zetler G (1982b) Caerulein and morphine: an attempt to differentiate their antinociceptive effects. Pharmacology 25:121–129
Zetler G (1983) Behavioral pharmacology of cholecystokinin (CCK) and analogs. Psychopharmacol Bull 19:347–351
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zetler, G., Mörsdorf, KH. Effects of ceruletide and cholecystokinin octapeptide on eating in mice. Naunyn-Schmiedeberg's Arch. Pharmacol. 325, 209–213 (1984). https://doi.org/10.1007/BF00495945
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00495945