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Absorption cytophotometry: Evaluation of three methods for the determination of DNA in feulgen stained nuclei

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Summary

Three methods for the evaluation of the relative amount of DNA per nucleus by absorption cytophotometry are compared. A combination of the two-wavelength method (Patau, 1952; Ornstein, 1952), the one-wavelength two-area method (Garcia, 1965) and the determination of transmission through nuclear plugs, is proposed in order to estimate systematic errors made by absorption cytophotometry.

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Authors and Affiliations

  1. Laboratory of Biochemistry and Laboratory of Plant Physiology, State University of Ghent, K.L. Ledeganckstraat 35, B-9000, Ghent, Belgium

    Patrick Van Oostveldt & Guido Boeken

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  1. Patrick Van Oostveldt
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  2. Guido Boeken
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Van Oostveldt, P., Boeken, G. Absorption cytophotometry: Evaluation of three methods for the determination of DNA in feulgen stained nuclei. Histochemistry 50, 147–159 (1976). https://doi.org/10.1007/BF00495825

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