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Freeze-fracture-autoradiography

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Summary

A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing. The advantages and limitations of the new method are discussed in terms of monolayer quality, contact, histochemography, resolution, freezing and recrystallisation artefacts.

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Authors and Affiliations

  1. I. Department of Physiology, University of Heidelberg, Im Neuenheimer Feld 326, D-6900, Heidelberg, Germany

    Eckhard Rix, August Schiller & Roland Taugner

Authors
  1. Eckhard Rix
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  2. August Schiller
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  3. Roland Taugner
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Additional information

This work was supported by the Deutsche Forschungsgemeinschaft and, as far as basic laboratory equipment is concerned, by the Bundesminister für Forschung und Technologie

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Rix, E., Schiller, A. & Taugner, R. Freeze-fracture-autoradiography. Histochemistry 50, 91–101 (1976). https://doi.org/10.1007/BF00495820

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  • DOI: https://doi.org/10.1007/BF00495820

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