Summary
A comparsion of the Feulgen hydrolysis curves and the chromatin compactness of the liver cell nuclei of young and old rats was made. It was found that the rate of DNA depurination and chromatin compactness are higher in the liver cell nuclei of old rats, both in di-and tetraploidal cells. The effect of fixation upon the course of the hydrolysis curves is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andersson, G.: Histochemical properties of spermatozoa and somatic cells. III. Depolimerization and extraction of DNA during Feulgen acid hydrolysis. Histochemistry 48, 315–324 (1976)
Andersson, G., Kjellstrand, P.: Exposure and removal of stainable groups during Feulgen acid hydrolysis of fixed chromatin at different temperetures. Histochemie 27, 165–172 (1971)
Andersson, G., Kjellstrand, P.: A study of DNA depolymerisation during Feulgen acid hydrolysis. Histochemistry 43, 123–130 (1975)
Beneke, G., Ervig, K., Schmitt, W.: Poliploidisierung und Heterochromatisierung von Sehnenzellkernen in Abhängigkeit vom Lebensalter. Beitr. Path 141, 19–32 (1970)
Berdyshev, G., Zhelabovskaya, S.: Composition, template properties and thermostability of liver chromatin from rats of various age at deproteinization by NaCl solutions. Exp. Gerontol. 7, 321–330 (1972)
Carriere, R.: The growth of liver parenchymal nuclei and its endocrine regulation. Int. Rev. Cytol. 25, 201–278 (1969)
Denkhaus, W., Kiefer, G., Meuter, P.: Untersuchungen über altersabhängige Veränderungen der Säurelabilität des Nuckleoproteids. Virchows Archiv Abt. B 3, 171–180 (1969)
Duijndam, W., van Duijn, P.: The influence of chromatin compactness on the stoichiometry of the Feulgen-Schiff procedure studied in model films. I. Theoretical kinetics and experiments with films containing isolated deoxyribonucleic acid. J. Histochem. Cytochem. 23, 882–890 (1975a)
Duijndam, W., van Duijn, P.: The influence of chromatin compactness on the stoichiometry of the Feulgen-Schiff procedure studied in model films. II. Investigations on films containing condensed or swollen chicken erythrocyte nuclei. J. Histochem. Cytochem. 23, 891–900 (1975b)
Graumann, W.: Zur Standardisierung der Schiff'schen Reagens. Z. wiss. Mikr. 61, 225–226 (1953)
Jordanov, J.: On the cytochemical demonstration of basic proteins in the cell nucleus, including the nucleolus. Acta histochem. (Jena) 55, 245–254 (1976)
Krug, H., von Wenk, H.: Veränderungen der Feulgen-Hydrolysekurve während des postnatalen Wachstums in Hippocampus der Ratte. Acta histochem. (Jena) 45, 305–321 (1974)
Rowiński, J., Pieńkowski, M., Abramczuk, J.: Area representation of optical density of chromatin in resting and stimulated lymphocytes as measured by means of Quantimet. Histochemie 32, 75–80 (1972)
Russel, A., Dowling, L., Herrmann, R.: Age-related differences in mouse liver DNA melting and hydroxylapatite fractionation. Gerontologia (Basel) 16, 159–171 (1970)
Sprenger, E., Böhm, N., Schaden, M., Sandritter, W.: Zur Kinetik der Feulgen-Hydrolyse bei Zellen junger und alter Ratten. Beitr. Path. 143, 59–69 (1971)
Author information
Authors and Affiliations
Additional information
This investigation was supported by grant 474/VI Committee of Cell Pathology, Polish Academy of Sciences
Rights and permissions
About this article
Cite this article
Myśliwski, A., Myśliwska, J. & Kmieć, Z. Age-related changes in chromatin of liver cell nuclei of different ploidity. Histochemistry 52, 91–96 (1977). https://doi.org/10.1007/BF00495818
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00495818