Summary
A statistically significant decrease in the intensity of catecholamine fluorescence of some carotid body glomus cells was observed after inhibition of the enzyme tyrosine hydroxylase by injection of 80 mg/kg α-methyl-paratyrosine. The intensity of the formaldehyde-induced fluorescence was measured in individual glomus cells. The maximum decrease in the intensity was observed 4 to 6h after the α-methyltyrosine injection. This suggests a rapid turnover in the catecholamines of the carotid body.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Böck, P.: Elektronenmikroskopische Untersuchungen zur Innervation des Glomus Caroticum beim Menschen. Z. mirk.-anat. Forsch. 82, 461–476 (1970)
Costa, M., Eränkö, O.: Histochemical correlates of cold-induced trans-synaptic induction in the rat superior cervical ganglion. Histochem. J. 6, 35–53 (1974)
Eränkö, O.: The practical histochemical demonstration of catecholamines by formaldehyde-induced fluorescence. J. roy. micr. Soc. 87, 259–276 (1967)
Fisz, M.: Probability theory and mathematical statistics. In: Wiley publications in statistics, p. 394. New York: John Wiley 1963
Hellström, S.: Morphometric studies of dense-cored vesicles in type I cells of rat carotid body. J. Neurocytol. 4, 77–86 (1975)
Hellström, S., Koslow, S.: Effects of glucocorticoid treatment on catecholamine content and ultrastructure of rat carotid body. Brain Res. 102, 245–255 (1976)
Korkala, O., Waris, T.: Ultrastructural changes in the rat carotid body in severe haemorrhagia. Cell Tiss. Res. 158, 355–362 (1975)
Levitt, M., Spector, S., Sjoerdsma, A., Udenfriend, S.: Elucidation of the rate-limiting step in norepinephrine biosynthesis in the perfused guinea-pig heart. J. Pharmacol. exp. Ther. 148, 1–8 (1965)
McDonald, D., Mitchell, R.A.: The innervation of glomus cells, ganglion cells, and blood vessels in the carotid body. A quantitative ultrastructural analysis. J. Neurocytol. 4, 177–230 (1975)
Moore, K., Dominic, J.: Tyrosine hydroxylase inhibitors. Fed. Proc. 30, 859–870 (1971)
Ploem, J.S.: The microscopic differentiation of colour of formaldehyde-induced fluorescence. Progr. Brain Res. 34, 27–38 (1971)
Sampson, S.R.: Innervation of the carotid body: another point of view. Fed. Proc. 31, 1383–1384 (1972)
Sharman, D.F.: The catabolism of catecholamines. Brit. med. Bull. 29, 110–119 (1972)
Spector, S., Sjoerdsma, A., Udenfriend, S.: J. Pharmacol. exp. Ther. 147, 86 (1965)
Van Orden, L.S. III, Burke, J.P., Geyer, M., Lodoen, F.V.: J. Pharmacol. exp. Ther. 174, 56–71 (1970)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Grönblad, M., Korkala, O. Loss of histochemically demonstrable catecholamines in the glomus cells of the carotid body after α-methyl-para-tyrosine treatment. Histochemistry 52, 85–90 (1977). https://doi.org/10.1007/BF00495817
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00495817