Summary
Copper has been localized in copper sulphate treated eggs of the slug Agriolimax reticulatus (Mü). This has been accomplished using a freeze-fracture technique, the freeze-dried halves of the fractured eggs being analysed in the scanning electron microscope, using both energy dispersive and wavelength dispersive X-ray microanalysis systems. The distribution of copper obtained using these methods has been compared with that achieved using a standard histochemical technique. Both techniques revealed that the copper is initially retained in the perivitelline membranes. The application of X-ray microanalysis to such studies is discussed.
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Barlow, D.J., Morgan, W.S.G.: Energy dispersive X-ray analysis as an autopsy technique for copper-caused fish mortality. Edax Editor 6, 96–99 (1976)
Bayne, C.J.: Observations on the composition of the layers of the egg of Agriolimax reticulatus, the grey field slug (Pulmonata, Stylomatophora). Comp. Biochem. Physiol. 19, 317–338 (1966)
Bayne, C.J.: Histochemical studies on the egg capsules of eight gastropod molluscs. Proc. malac. Soc. Lond. 38, 199–212 (1968)
Carrick, R.: The life history and development of Agriolimax agrestis L., the grey field slug. Trans. roy. Soc. Edinb. 59, 563–596 (1938)
Goldfischer, S., Moskal, J.: Electron probe microanalysis of liver in Wilson's disease. Amer. J. Path. 48, 305–315 (1966)
Kandiah, K.: Some limitations of energy dispersive X-ray spectrometers. In: Physical aspects of electron microscopy and microbeam analysis (B.M. Siegel and D.R. Beaman, eds.). New York: John Wiley 1975
Russ, J.C.: Microanalysis of thin sections in the TEM and STEM using energy dispersive X-ray analysis. In: Electron microscopy and cytochemistry (E. Wisse, W.Th. Daems, I. Molenaar, P. Van Duijn, eds.). Amsterdam: North Holland 1973
Stephenson, J.W.: A review of the biology and ecology of slugs of agricultural importance. Proc. malac. Soc. Lond. 38, 169–178 (1968)
Tousimis, A.J., Adler, I.: Electron probe X-ray microanalyzer study of copper within Descemet's membrane of Wilson's disease. J. Histochem. Cytochem. 11, 40–47 (1963)
Waterhouse, D.F.: Studies on the physiology and toxicology of blowflies X. A histochemical examination of the distribution of copper in Lucilia cuprina. Austral. Counc. Sci. Indust. Res. Bull. 191, 1–39 (1945)
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This research was supported by the Agricultural Research Council (G.B.), Grant No. AG 72/13
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Ryder, T.A., Bowen, I.D. The use of X-ray microanalysis to demonstrate the uptake of the molluscicide copper sulphate by slug eggs. Histochemistry 52, 55–60 (1977). https://doi.org/10.1007/BF00495814
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DOI: https://doi.org/10.1007/BF00495814