, Volume 84, Issue 3, pp 271–274 | Cite as

Simultaneous electron microscopic detection of colloidal gold labeled ligand and adenylate cyclase

Influence of concanavalin-A on adenylate cyclase activity of fibroblasts
  • F. Sudár
  • G. Csaba


Concanavalin-A-colloidal gold (Con-A-G) complex and adenylate cyclase activity were detected simultaneously in electron microscopic preparations of human fibroblast cultures, by a combined histochemical technique. The colloidal gold particles appeared as round bodies which could be readily differentiated from the amorphous product of the adenylate cyclase enzyme reaction. The combined technique makes possible the simultaneous visualization of the bound ligand (i.e. of its binding site), and of the enzyme activated by the lignad. Treatment of the cells with Con-A accounted for a considerable increase in intracellular adenylate cyclase activity. The activity increase was disproportionally greater than the amount of bound ligand, and it also appeared in localizations showing no indication of ligand binding. Treatment of the fibroblasts with Con-A was followed by internalization of the lignand and the enzyme inside at least seemingly segregated vesicles.


Adenylate Cyclase Gold Particle Colloidal Gold Adenylate Cyclase Activity Fibroblast Culture 
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  1. Bancroft JD, Stevens A (1982) Theory and practice of histological techniques. Churchill-Livingstone, Edinburgh New YorkGoogle Scholar
  2. Costlow ME, Gallagher PE (1979) Relationship of prolactin receptors to concanavalin A binding. Biochim Biophys Acta 587:192–201Google Scholar
  3. Csaba G, Kovács P (1982) Histamine-lectin and insulin-lectin binding site overlaps in Tetrahymena. Cell Mol Biol 28:153–158Google Scholar
  4. Feller M, Behnke D, Gruenstein E (1979) Relationship between lectin monosaccharide specificity and binding to the plasma membrane of human fibroblasts. Biochim Biophys Acta 586:315–329Google Scholar
  5. Fletcher WA, Greenan JRT (1985) Receptor mediated action without receptor occupancy. Endocrinology 116:1660–1662Google Scholar
  6. Frens G (1972) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature (Phys Sci) 241:20–22Google Scholar
  7. Gilman AG (1970) A protein binding assay for adenosine 3′5′-cyclic monophosphate. Proc Natl Acad Sci USA 67:305–312Google Scholar
  8. Hollenberg MD (1985) Examples of homospecific and heterospecific receptor regulation. TIPS 6:242–245Google Scholar
  9. Kovács P, Csaba G, László V (1984) Study of the imprinting and overlap of insulin and Concanavalin A at the receptor level in a protozoan (Tetrahymena) model system. Acta Physiol Hung 64:19–23Google Scholar
  10. Reik GL, Petzold JA, Higgins J, Greengard P, Barrnett RJ (1968) Hormone-sensitive adenyl cyclase: cytochemical localization in rat liver. Science 168:382–384Google Scholar
  11. Sandra A, Leon MA, Przybilsky RJ (1979) Reversla by insulin of Concanavalin A inhibition of myotube formation and evidence for common binding sites. Endocrinology 105:391–401Google Scholar
  12. Schrével J, Gros D, Monsigny M (1981) Cytochemistry of cell glycoconjugates. Gustav Fischer, Stuttgart New YorkGoogle Scholar
  13. Sharon NT, Lis A (1975) Use of lectins for the study of membranes. In: Korn E (ed) Methods of membrane biology, vol 3. Plenum Press, New YorkGoogle Scholar
  14. Suzuki T, Makino H, Kanatsuka A, Osegawa M, Yoshida S, Sakamoto Y (1984) Activation of insulin sensitive phosphodiesterase by lectins and dextran complex in rat fat cells. Metabolism 33:572–576Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • F. Sudár
    • 1
  • G. Csaba
    • 1
  1. 1.Department of BiologySemmelweis University of MedicineBudapestHungary

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