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Histochemistry

, Volume 84, Issue 3, pp 251–255 | Cite as

Histochemical and immunohistochemical profile of pink muscle fibres in some teleosts

  • E. Mascarello
  • M. G. Romanello
  • P. A. Scapolo
Article

Summary

The pink muscle of several Teleosts was examined immunohistochemically using antisera specific for the myosins of red and white muscle, and histochemically using various methods for demonstrating myosin ATPase (in ATPase) activity.

In the catfish the pink muscle consists of 2 different layers of fibres. The superficial layer has a low mATPase activity after both acid and alkali pre-incubation, whereas the deeper layer has a high mATPase activity after acid and alkali pre-incubation, being more resistent to these conditions even than is the white muscle.

In the trout the pink muscle is composed of fibres with the same mATPase activity as in the superficial pink muscle of the catfish, whereas in the rock goby, goldfish, mullet and guppy the pink muscle is like the deep pink layer of the catfish.

Immunohistochemically the fibres of the pink muscle behave like the white muscle fibres except in the guppy and rock goby in which at the level of the lateral line there occurs a transition zone between red and pink fibres. The fibres of this region react with both anti-fast and (to a lesser extent) anti-slow myosin antisera, and have a mATPase activity which, going from the superficial to the deeper fibres, gradually loses the red muscle characteristics to acquire those of the main pink muscle layer.

Keywords

Muscle Fibre Transition Zone Deep Layer Lateral Line Muscle Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Akster HA (1983) A comparative study of fibre type characteristics and terminal innervation in head and axial muscle of the carp (Cyprinus carpio L.). A histochemical and electron microscopical study. Neth J Zool 33:164–188Google Scholar
  2. Akster HA, Osse JWM (1978) Muscle fibre types in head muscle of the perch (Perca fluviatilis L.), Teleostei. A histochemical and electro-myographical study. Neth J Zool 28:94–110Google Scholar
  3. Bokdawala FD (1967) A histochemical study of fat in the red and white fibres of fish skeletal muscle. J Anim Morphol Physiol 14:231–241Google Scholar
  4. Bokdawala FD, George JC (1967a) A histochemical study of the red and white muscles of the carp, Cirrhina mrigala. J Anim Morphol Physiol 14:60–68Google Scholar
  5. Bodkawala FD, George JC (1967b) A quantitative study of fat, glycogen, lipase and succinic dehydrogenase in fish muscle. J Anim Morphol Physiol 14:223–230Google Scholar
  6. Carpenè E, Veggetti A, Mascarello F (1982) Histochemical fibre types in the lateral muscle of fishes in fresh, brackish and salt water. J Fish Biol 20:379–396Google Scholar
  7. Connell JJ (1960) Studies on the proteins of fish skeletal muscle. 7. Denaturation and aggregation of cod myosin. Biochem J 75:530–538Google Scholar
  8. George JC, Bokdawala FD (1964) Cellular organization and fat utilization in fish muscle. J Anim Morphol Physiol 11:124–132Google Scholar
  9. Johnston IA, Lucking M (1978) Temperature induced variation in the distribution of different types of muscle fibre in the Goldfish (Carassius auratus). J Comp Physiol 124:111–116Google Scholar
  10. Johnston IA, Patterson S, Ward P, Goldspink G (1974) The histochemical demonstration of myofibrillar adenosine tri phosphatase activity in fish muscle. Can J Zool 52:871–877Google Scholar
  11. Johnston IA, Ward PS, Goldspink G (1975a) Studies on the swimming musculature of the rainbow trout. I. Fibre types. J Fish Biol 7:451–458Google Scholar
  12. Johnston IA, Walesby NJ, Davison W, Goldspink G (1975b) Temperature adaptation in myosin of Antartic fish. Nature 254:74–75Google Scholar
  13. Johnston IA, Davison W, Goldspink G (1977) Energy metabolism of carp swimming muscles. J Comp Physiol 203–216Google Scholar
  14. Kronnie G te, Tatarczuch L, van Raamsdonk W, Kilarski W (1983) Muscle fiber types in the myotome of stickleback, Gasterosteus aculeatus L.; a histochemical, immunohistochemical and ultrastructural study. J Fish Biol 22:303–316Google Scholar
  15. Mosse PRL, Hudson RCL (1977) The functional roles of different muscle fibre types identified in the myotomes of marine Teleosts: a behavioural anatomical and histochemical study. J Fish Biol 11:417–430Google Scholar
  16. Ogata T, Mori M (1964) Histochemical study of oxidative enzymes in Vertebrate muscles. J Histochem Cytochem 12:171–182Google Scholar
  17. Patterson S, Johnston JA, Goldspink G (1975) A histochemical study of the lateral muscles of five Teleost species. J Fish Biol 7:159–166Google Scholar
  18. Raamsdonk W van, Pool CW, teKronnie G (1978) Differentiation of muscle fiber types in the Teleost Brachydanio rerio. Anat Embryol 153:137–155Google Scholar
  19. Raamsdonk W van, teKronnie G, Pool CW, van de Laarse W (1980) An immune histochemical and enzymatic characterization of the muscle fibres in myotomal muscle of the Teleost Brachydanio rerio, Hamilton, Buchanan. Acta Histochem 67:200–216Google Scholar
  20. Raamsdonk W van, van t'Veer L, Veeken K, teKronnie G, de Jager S (1982) Fiber type differentiation in fish. Mol Physiol 2:31–47Google Scholar
  21. Rowlerson A, Scapolo PA, Mascarello F, Carpenè E, Veggetti A (1985) Comparative study of myosin present in the lateral muscle of some fish: species variations in myosin isoforms and their distribution in red, pink and white muscle. J Muscle Res Cell Motil 6:601–640Google Scholar
  22. Snow DH, Billeter R, Mascarello F, Carpenè E, Rowlerson A, Jenny E (1982) No classical type IIB fibres in dog skeletal muscle. Histochemistry 75:53–65Google Scholar
  23. Tatarczuch L, Kilarski W (1982) Histochemical analysis of muscle fibres in myotome of Teleost fishes (Carassius auratus, Gibelio). Folia Histochem Cytochem 20:143–170Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • E. Mascarello
    • 1
  • M. G. Romanello
    • 1
  • P. A. Scapolo
    • 2
  1. 1.Istituto di Anatomia degli Animali Domestici con Istologia ed EmbriologiaUniversità degli Studi di MilanoMilanoItalia
  2. 2.Istituto di Anatomia degli Animali Domestici con Istologia ed EmbriologiaUnversità degli Studi di BolognaBolognaItalia

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