Summary
The ovaries of 70 mature Mongolian gerbils (Meriones unguiculatus) were investigated morphologically and enzyme histochemically for the appearance of acid phosphatase, non-specific esterases, succinate dehydrogenase and thiamine pyrophosphatase. In the oocyte two particular enzyme active zones exist depending on the state of development. In young oocytes acid phosphatase, succinate dehydrogenase and thiamine pyrophosphatase can be found only in the perinuclear zone. From the late secondary follicle on, activity of acid phosphatase, succinate dehydrogenase and non-specific esterases can be detected only in a peripheral area of cytoplasm, whereas thiamine pyrophosphatase is present in the entire ooplasm.
In the follicular epithelium a different pattern of enzyme distribution suggests a functional differentiation of the epithelial cells during folliculogenesis.
Similar content being viewed by others
References
Adams EC, Hertig AT (1964) Studies on guinea pig oocyte. I. Electron microscopic observations on the development of cytoplasmic organelles in oocytes of primordial and primary follicles. J Cell Biol 21:397–427
Allen JM, Slater JJ (1961) A cytochemical study of Golgi associated thiamine pyrophosphatase in the epididymis of the mouse. J Histochem Cytochem 9:418–423
Anderson E, Beams HW (1960) Cytological observations on the fine structure of the guinea-pig ovary with special reference to the oogenium, primary oocyte, and associated follicle cells. J Ultrastruct Res 5:432–446
Austin CR (1956) Cortical granules in hamster eggs. Exp Cell Res 10:533–540
Balbiani EG (1893) Centrosome et “Dotterkern”. J Anat Physiol (Paris) 29:145–179
Banon P, Brandes D, Frost J (1964) Lysosomal enzymes in the rat ovary and endometrium during the estrous cycle. Acta Cytol 8:416–425
Barka T, Anderson PJ (1962) Histochemical methods for acid phosphatase using hexazonium-p-rosanilin as coupler. J Histochem Cytochem 10:741–753
Bjersing L (1977) Ovarian histochemistry. In: Zuckermann L, Weir B (eds) The ovary. Academic Press, New York London, pp 303–368
Blanchette EJ (1961) A study of the fine structure of the rabbit primary oocyte. J Ultrastruct Res 5:349–363
Brachet J (1960) The biochemistry of development. Pergamon Press, London Oxford New York
Bulmer D (1964) The histochemical distribution of certain ovarian enzymes. J Anat 98:27–36
Burkl W (1965) Zur kausalen Genese der Follikelatresie. Arch Gynäkol 200:689–698
Burkl W, Thiel-Bartosch E (1967) Elektronenmikroskopische Untersuchungen über die Granulosa atresierender Tertiärfollikel bei der Ratte. Arch Gynäkol 204:238–250
Burr JH, Davis JI (1951) The vascular system of the rabbit ovary and its relationship to ovulation. Anat Rec 111:273–297
Burstone MS (1958) Histochemical demonstration of acid phosphatase with naphthol-AS-Phosphate. J Natl Cancer Inst 21:523–539
Davis BJ, Ornstein L (1959) High resolution enzyme localization with a new diazo reagent, “hexazonium pararosanilin”. J Histochem Cytochem 7:297–298
DeDuve CM (1963) General properties of lysosomes. The lysosomes concept. In: Lysosomes, Ciba Foundation Symp Edit AVS des Reuck and MP Cameron. Little Brown, Boston, Comp, pp 1–31
Hadek R (1963) Electron microscope study on primary liquor folliculi secretion in the mouse ovary. J Ultrastruct Res 9:445–458
Hertig AT (1968) The primary human oocyte: Some observations on the fine structure of Balbiani's vitelline body and the origin of the annulate lamellae. Am J Anat 122:107–138
Jirasek JE (1961) Zur Lokalisation der unspezifischen Esterase im menschlichen Ovarium. Arch Gynäkol 196:359–364
Kar AB (1962) Incipient follicular atresia and acid phosphatase activity in ovary and serum of rats. J Sci Indust Res 21:231–237
Kling D (1981) Total atresia of the ovaries of Tilapia leucosticta after intoxication with the insecticide Lebaycid®. Experientia 37:73–74
Korfsmeier KH (1979) Acid phosphatase in the guinea pig oocytes. Histochemistry 63:123–127
Korfsmeier KH (1980a) Lysosomal enzymes in oocytes of the mongolian gerbil, Meriones unguiculatus. Histochemistry 70:91–93
Korfsmeier KH (1980b) Lysosomale Enzyme in Säugereizellen. Verh Anat Ges 74:449–450
Krauskopf C (1968) Elektronenmikroskopische Untersuchungen über die Struktur der Oocyte und des 2 Zellstadiums beim Kaninchen. I Oocyte. Z Zellforsch 92:275–295
Lobel BL, Rosenbaum RM, Wendler-Deane H (1961) Enzymic correlates of physiological regression of follicles and corpora lutea in the ovaries of normal rat. Endocrinology 68:232–247
Lojda ZP, Gossrau R, Schiebler TH (1976) Enzymhistochemische Methoden. Springer; Berlin Heidelberg New York
Lojda ZP (1965) Remarks on histochemical detection of dehydrogenase. II. Intracellular localization. Folia Morphol (Prague) 3:84–96
McReynolds H, Siraki CM, Bramson PH, Pollock RJ, jr (1973) Smooth muscle-like cells in ovaries of the hamster and gerbil. Z Zellforsch 140:1–8
Mossman HW, Duke KL (1973) Comparative morphology of the mammalian ovary. University of Wisconsin Press, Wisconsin
Novikoff AB, Goldfischer S (1961) Nucleoside diphosphatase activity in the Golgi-apparatus and its usefullness for cytological studies. Proc Natl Acad Sci USA 47:802–810
Oakberg EF (1979) Follicular growth and atresia in the mouse. In vitro 15:41–49
Odor DL (1960) Electron microscopy study on ovarian oocytes and unfertilized tubal ova in the rat. J Biophys Biochem Cytol 7:567–674
Palade GE (1952) A study of fixation for electron microscopy. J Exp Med 95:285–298
Peluso JJ, England-Charlesworth C, Bolender DL, Steger RW (1980) Ultrastructural alterations associated with the initiation of follicular atresia. Cell Tissue Res 211:105–115
Richardson UE, Jarett L, Finke EH (1960) Embedding in expoxy resins for ultra thin sectioning in electron microscopy. Stain Technol 35:313–323
Roth ThF, Porter KR (1964) Yolk protein uptake in the oocyte of the mosquito Aedes aegyptii L. J Cell Biol 20:313–332
Rune G (1982) Zisternen und tubuläre Systeme in der Oocyte von Meriones unguiculatus Verh Anat Ges 76: (in press)
Schmidtler W (1980) Lysosomale Enzyme in der Oogenese und Follikulogenese des Meerschweinchens. Histochemistry 70:77–90
Sotelo JR (1959) An electron microscope study of the cytoplasmic and nuclear components of rat primary oocytes. Z Zellforsch 50:749–765
Sotelo JR, Porter KR (1959) An electron microscope study on the rat ovum. J Biophys Biochem Cytol 5:327–342
Spanel-Borowski K (1981) Morphological investigations on follicular atresia in Canine ovaries. Cell Tissue Res 214:155–168
Szollosi D (1962) Cortical granules: A general feature of mammalian eggs. Fertil Steril 4:223–224
Taki I, Hamanaka N (1966) Histochemical observations of enzymatic pattern in human ovaries. Am J Obstet Gynecol 96:388–399
Thiessen D, Yahr P (1977) The gerbil in behavioral investigations. University of Texas Press, Austin. London
Trujillo-Cenoz O, Sotelo JR (1959) Relationship of the ovular surface with follicle cells and origin of the zona pellucida in rabbit oocytes. J Biophys Biochem Cytol 5:347–350
Vazquez-Nin GH, Sotelo JR (1967) Electron microscope study of the atretic oocytes of the rat. Z Zellforsch 80:518–533
Wartenberg H (1964) Experimentelle Untersuchungen über dic Stoffaufnahme durch Pinocytose während der Vitellogenese der Amphibianoocyten. Z Zellforsch 63:1004–1019
Watzka M (1957) Das Ovarium. In: Handbuch der mikroskopischen Anatomie des Menschen. Harn- und Geschlechtsapparat. Ergänzungen zu Bd VII/1, Teil 3: Weibliche Geschlechtsorgane. Springer, Berlin Göttingen Heidelberg
Weakley B (1966) Electron microscope of the oocyte and granulosa cells in the developing ovarian follicles of the golden hamster (Mesocricetus auratus). J Anat 100:503–531
Zamboni L, Mastroanni L (1966) Electron microscope studies on rabbit ovary. I. The follicular oocyte. J Ultrastruct Res 14:95–117
Zerbian K (1966) Über das histochemische Verhalten einiger Enzyme bei der Follikelatresie im Ovarium des Meerschweinchens. Acta Histochem 23:303–321
Author information
Authors and Affiliations
Additional information
This investigation was supported by the Deutsche Forschungsgemeinschaft
Rights and permissions
About this article
Cite this article
Rune, G. Histochemical investigation of the folliculogenesis in the ovary of the Mongolian gerbil (Meriones unguiculatus). Histochemistry 80, 299–306 (1984). https://doi.org/10.1007/BF00495781
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00495781