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Histochemistry

, Volume 80, Issue 3, pp 273–276 | Cite as

Substance P-like immunoreactive trigeminal ganglion cells supplying the cornea

  • J. I. Lehtosalo
Article

Summary

Trigeminal ganglion cells supplying the cornea were traced with intra-axonally transported horseradish peroxidase and, subsequently studied for the presence of substance P-like immunoreactivity. Approximately 0%–30% of trigeminal ganglion cells contained immunoreactive substance P. These cells were of a small size (15–50 μm in diameter) and were distributed throughout the ganglion. The ganglion cells supplying the cornea were of a relatively small size as well but were confined to the anteromedial part of the ganglion. Some of these cells were found to contain immunoreactive substance P.

Keywords

Public Health Horseradish Peroxidase Ganglion Cell Trigeminal Ganglion Immunoreactive Substance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Belmonte C, Giraldez F (1981) Responses of cat corneal sensory receptors to mechanical and thermal stimulation. J Physiol 321:355–368Google Scholar
  2. Bill A, Stjernschantz J, Mandahl A, Brodin E, Nilsson G (1979) Substance P: Release on trigeminal nerve stimulation, effects in the eye. Acta Physiol Scand 106:371–373Google Scholar
  3. Brann MR, Emson PC (1980) Microiontophoretic injection of fluorescent tracer combined with simultaneous immunofluorescence histochemistry for the demonstration of efferents from the caudate-putamen projecting to the globus pallidus. Neurosci Lett 16:61–66Google Scholar
  4. Brimijoin S, Lundberg JM, Brodin E, Hökfelt T, Nilsson G (1980) Axonal transport of substance P in the vagus and sciatic nerves of the guinea pig. Brain Res 191:443–457Google Scholar
  5. Cuello AC, Del Fiacco M, Paxinos G (1978) The central and peripheral ends of the substance P-containing sensory neurones in the rat trigeminal system. Brain Res 152:499–509Google Scholar
  6. Harmer A, Keen P (1982) Synthesis, and central and peripheral axonal transport of substance P in a dorsal root ganglion — nerve preparation in vitro. Brain Res 231:379–385Google Scholar
  7. Hoyes AD, Barber P (1976) Ultrastructure of the corneal nerves in the rat. Cell Tissue Res 172:133–144Google Scholar
  8. Hökfelt T, Kellerth JO, Nilsson G, Pernow B (1975) Substance P: localization in the central nervous system and in some primary sensory neurons. Science 190:889–890Google Scholar
  9. Hökfelt T, Terenius L, Kuypers HGJM, Dann O (1979) Evidence for enkephalin immunoreactive neurons in the medulla oblongate projecting to the spinal cord. Neurosci Lett 14:55–60Google Scholar
  10. Keen P, Tullo AB, Blyth WA, Hill TJ (1982) Substance P in the mouse cornea: effects of chemical and surgical denervation. Neurosci Lett 29:231–235Google Scholar
  11. Kuypers HGJM, Bentivoglio M, Van der Kooy D, Catsman-Berrevoets CE (1979) Retrograde transport of bisbenzimide and propidium iodide through axons to their parent cell bodies. Neurosci Lett 12:1–7Google Scholar
  12. Malmgren L, Olsson Y (1978) A sensitive method for histochemical demonstration of horseradish peroxidase in neurons following retrograde axonal transport. Brain Res 148:279–294Google Scholar
  13. Marfurt CF (1981) The somatotopic organization of the cat trigeminal ganglion as determinated by the horseradish peroxidase technique. Anat Rec 201:105–118Google Scholar
  14. Matsuda H (1968) Electron microscopic study on the corneal nerve with special reference to the nerve endings. Acta Soc Ophthal Jpn 72:880–893Google Scholar
  15. Miller A, Costa M, Furness JB, Chubb IW (1981) Substance P immunoreactive sensory nerves supply the rat iris and cornea. Neurosci Lett 23:243–249Google Scholar
  16. Neuhuber W, Groh V, Gottschall J, Lelio MR (1981) The cornea is not innervated by substance P-containing primary afferent neurons. Neurosci Lett 22:5–9Google Scholar
  17. Olgart L, Gazelius B, Brodin E, Nilsson G (1977) Release of substance P-like immunoreactivity from the dental pulp. Acta Physiol Scand 101:510–512Google Scholar
  18. Panneton WM, Burton H (1981) Corneal and periocular representation within the trigeminal sensory complex in the cat studied with transganglionic transport of horseradish peroxidase. J Comp Neurol 199:327–344Google Scholar
  19. Piekut DT, Casey SM (1983) Penetration of immunoreagents in vibratome-sectioned brain: A light and electron microscope study. J Histochem Cytochem 31:669–674Google Scholar
  20. Sawchanko PE, Swanson LW (1981) A method for tracing biochemically defined pathways in the central nervous system using combined fluorescence retrograde transport an immunohistochemical techniques. Brain Res 210:31–51Google Scholar
  21. Soloway MR, Stjernschantz J, Sears M (1981) The miotic effect of substance P on the isolated rabbit iris. Invest Ophthalmol Vis Sci 20:47–52Google Scholar
  22. Sternberger LA, Hardy PH, Curculis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry. J Histochem Cytochem 18:315–333Google Scholar
  23. Stjernschantz J, Geijer C, Bill A (1979) Electrical stimulation of the fifth cranial nerve in rabbits: effects on ocular blood flow, extravascular albumin content and intraocular pressure. Exp Eye Res 28:229–238Google Scholar
  24. Stjernschantz J, Sears M, Stjernschantz L (1981) Intraocular effects of substance P in the rabbit. Invest Ophthalmol Vis Sci 20:53–60Google Scholar
  25. Stjernschantz J, Gregerson D, Bausher L, Sears M (1982) Enzymelinked immunosorbent assay of substance P: a study in the eye. J Neurochem 38:1323–1328Google Scholar
  26. Tervo K, Tervo T, Eränkö L, Eränkö O, Cuello AC (1981) Immunoreactivity for substance P in the Gasserian ganglion, ophthalmic nerve and anterior segment of the rabbit eye. Histochem J 13:435–443Google Scholar
  27. Unger WG, Butler JM, Cole DF, Bloom SR, McGregor GP (1981) Substance P, vasoactive intestinal polypeptide (VIP) and somatostatin levels in ocular tissues of normal and sensorily denervated rabbit eyes. Exp Eye Res 32:797–801Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • J. I. Lehtosalo
    • 1
  1. 1.Department of AnatomyUniversity of HelsinkiHelsinki 17Finland

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