Summary
In the intercellular matrix of the granulosa layer of the mouse ovarian follicles, ultrastructural features of acidic complex carbohydrates have been studied by means of dialyzed iron (DI) staining in combination with procedures of digestion with Streptomyces and testicular hyaluronidases. In the intercellular matrix, DI reactive structures containing acidic complex carbohydrates consist of layers of a variable thickness coating the plasma membrane of the granulosa cells and reticular elements distributed in the spaces between the cells. The latter exists in two appearances; one is clumped masses of irregular shapes and different sizes, whereas the other being filamentous figures radiating from the masses. The effects of digestion with Streptomyces and testicular hyaluronidases upon the DI staining of the tissues indicate that the DI reactive structures in the intercellular matrix contain at least three types of acidic complex carbohydrates; hyaluronic acid, isomeric chondroitin sulfates and other acidic glycosaminoglycans. The histophysiological activities played by these particular complex carbohydrates have been briefly discussed.
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Balazs, E.A., Gibbs, D.A.: The rheological properties and biological function of hyaluronic acid. In: Chemistry and molecular biology of the intercellular matrix (ed. E.A. Balazs), Vol. 3, pp. 1241–1253. London and New York: Academic Press 1970
Bjersing, L.: Ovarian histochemistry In: The ovary (eds. L. Zuckerman and B.J. Weir), Vol. 1, pp. 303–391. London and New York: Academic Press 1977
Hardin, J.H., Spicer, S.S.: Ultrastructural localization of dialyzed iron reactive mucosubstance in rabbit heterophils, basophils and eosionphils. J. Cell Biol. 48, 368–386 (1971)
Hoage, T.R., Cameron, I.L.: Folliculogenesis in the ovary of the mature mouse. A radioautographic study. Anat. Rec. 184, 699–710 (1976)
Hoshino, M., Yamada, K.: Effects of digestion with chondroitinases upon mucosaccharide stainings of rabbit cartilages as revealed by electron microscopy. Histochemie 32, 221–229 (1972)
Hoshino, M.: The histochemistry of hyaluronic acid and related mucosaccharides in the cerebral and other arteries of the dog. Nagoya J. Med. Sci. 37, 33–46 (1974)
Jackson, F.S.: Connective tissue cells. In: The cell (eds. J. Brachet and A.E. Mirsky) Vol. VI, pp. 387–520. New York and London: Academic Press 1964
Jacoby, F.: Ovarian histochemistry. In: The ovary (ed. S. Zuckerman) Vol. 1, pp. 189–245. New York and London: Academic Press 1962
Leppi, T.J., Stoward, P.J.: On the use of testicular hyaluronidase for identifying acid mucins in tissue sections. J. Histochem. Cytochem. 13, 406–407 (1965)
Luft, J.H.: Improvements in Epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9, 409–414 (1961)
Ogston, A.G.: The biological functions of the glycosaminoglycans. In: Chemistry and molecular biology of the intercellular matrix (ed. E.A. Balazs), Vol. 3, pp. 1231–1240. London and New York: Academic Press 1970
Ohya, T., Kaneko, Y.: Novel hyaluronidase from Streptomyces. Biochim. Biophys. Acta 198, 607–609 (1970)
Quintarelli, G., Vocaturs, A., Bellocci, M., Roden, L., Ippolito, E., Baker, J.R.: Preliminary ultrastructural demonstration of hyaluronic acid-proteoglycan interaction in cartilage matrix (1). Am. J. Anat. 140, 433–438 (1974)
Shinohara, H.: On glycoproteins. Biochemistry 49, 1219–1237 (1977)
Tadano, Y., Yamada, K.: The histochemistry of complex carbohydrates in the ovarian follicles of adult mice. Histochemistry 57, 203–215 (1978)
Toole, B.P., Okayama, M., Orkin, R.W., Yoshimura, M., Muto, M., Kaji, A.: Developmental roles of hyaluronate and chondroitin sulfate-proteoglycans. In: Cell and tissue interactions (eds. J.W. Lash and M.M. Burger) pp. 139–154. New York: Raven Press 1977
Weissmann, B., Hadjiioannon, S., Tornheim, J.: Oligosaccharase activity of β-N-acetyl-D-glucosaminidase of beef liver. J. Biol. Chem. 239, 59–63 (1964)
Wetzel, M.G., Wetzel, B.K., Spicer, S.S.: Ultrastructural localization of acid mucosubstances in the mouse colon with iron containing stains. J. Cell Biol. 30 299–315 (1966)
Yamada, K.: Histochemical and cytochemical methods for the study of mucosaccharides. Protein Nucleic Acid Enzyme 17, 775–790 (1972)
Yamada, K., Hirano, K.: The histochemistry of hyaluronic acid-containing mucosubstances. J. Histochem. Cytochem. 21, 469–472 (1973)
Yamada, K.: The effect of digestion with Streptomyces hyaluronidase upon certain histochemical reactions of mucosaccharide-containing tissues. J. Histochem. Cytochem. 21, 794–803 (1973a)
Yamada, K.: Cytochemistry (Polysaccharides). In: Textbook of techniques for electron microscopy (Nagoya), pp. 134–147. Nagoya: Koketsu-shuppan Printing Co. 1973b
Yamada, K., Shimizu, S.: The use of Streptomyces hyaluronidase in connective tissue specimens for electron microscopic histochemistry of acid mucosaccharides. Connect. Tissue 7, 61–71 (1975)
Yamada, K., Shimizu, S.: Electron microscopic histochemistry of acid mucosaccharides in intercellular matrix. Connect. Tissue 10 (Suppl.), 40 (1978)
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Tadano, Y., Yamada, K. Ultrastructural features of acidic complex carbohydrates in the intercellular matrix of the ovarian follicles in adult mice. Histochemistry 60, 125–133 (1979). https://doi.org/10.1007/BF00495748
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DOI: https://doi.org/10.1007/BF00495748