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Correlation of individual skeletal muscle fibres from “semithin” sections stained with p-phenylene-diamine and histochemical sections incubated for myofibrillar ATP-ase

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Summary

Muscle fibres in transverse “semithin” sections of osmium-fixed and plastic embedded muscle tissue stained with p-phenylene-diamine has been classified into three types (M, INT and H) and correlated to the same fibres in neighboring sequential preincubated myofibrillar ATP-ase sections. A close correlation, but no equality was found between the fibre types classified according to the ATP-ase and the p-phenylene-diamine methods. On the average, the content of mitochondria and fat droplets was highest in the ATP-ase classified type I fibres (dominated by M fibres), and higher in the endurance trained than in the untrained subjects.

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Authors and Affiliations

  1. Laboratory of Physiology, Norwegian College of Physical Education and Sport, Kringsjå, Box 40, Oslo 8, Norway

    F. Ingjer

  2. Anatomical Institute, University of Oslo, Norway

    F. Ingjer

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  1. F. Ingjer
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Ingjer, F. Correlation of individual skeletal muscle fibres from “semithin” sections stained with p-phenylene-diamine and histochemical sections incubated for myofibrillar ATP-ase. Histochemistry 60, 107–111 (1979). https://doi.org/10.1007/BF00495734

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  • DOI: https://doi.org/10.1007/BF00495734

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