Summary
Electron microscopic cytochemical studies of cells of the gastric glands of the stomach reveal enzymatic sites for carbonic anhydrase activity within cells of the gastric glands of hamsters and humans not previously described. The localization of the carbonic anhydrase activity is made apparent by reaction products of lead salts. Numerous electron dense precipitates are evident as discrete circular and irregular profiles and are situated on the outer surface of the mitochondria of the parietal cells. Smaller amounts of the reaction products are seen within the granular endoplasmic reticulum, mitochondrial matrix and on the outer surface of the mitochondria of the chief cells.
Similar content being viewed by others
References
Chung, A.: Carbonic anhydrase histochemistry: evidence for non-enzymatic reaction and artifact production. Histochemistry (in press, 1974)
Cross, S. A. M.: Ultrastructural localisation of carbonic anhydrase in rat stomach parietal cells. Histochemie 22, 219–225 (1970)
Davenport, H. W.: Carbonic anhydrase inhibition and physiological function. In: Enzymes and drug action, eds. J. L. Mongar and A. V. S. de Reuck, p. 16–27. Boston: Ciba Foundation Symposium, Little Brown & Co. 1962
Häusler, G.: Zur Technik und Spezifitat des histochemischen Carbonanhydrasenachweises im Modellversuch und in Gewebsschnitten von Rattennieren. Histochemie 1, 29–47 (1958)
Hansson, H. P. J.: Histochemical demonstration of carbonic anhydrase activity. Histochemie 11, 112–128 (1967)
Hopsu, V. K., Arstila, A. W., Helminen, H.: Studies on the electron microscopy of arylsulphatase and carbonic anhydrase activities. J. Histochem. Cytochem. 14, 762 (1966)
Karler, P. K., Woodbury, D. M.: Intracellular distribution of carbonic anhydrase. Biochem. J. 75, 538–543 (1960)
Kurata, Y.: Histochemical demonstration of carbonic anhydrase activity. Stain Technol. 28, 231–233 (1953)
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)
Meldrum, N. U., Roughton, F. J. W.: Some properties of carbonic anhydrase, the CO2 enzyme present in blood. J. Physiol. (Lond.) 75, 15P-16P (1932)
Meldrum, N. U., Roughton, F. J. W.: Carbonic anhydrase. Its preparation and properties. J. Physiol. (Lond.) 80, 113–142 (1933)
Sabatini, D., Bensch, K., Barrnett, R. J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963)
Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965)
Winborn, W. B., Seelig, L. L., Jr.: Osmium impregnation and carbonic anhydrase localization studies in parietal cells of the stomach. Proc. Amer. Soc. Cell Biol., p. 327 (1971)
Yokota, S.: Electron microscopic demonstration of carbonic anhydrase activity in mouse liver cells. Histochemie 19, 255–261 (1969)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Winborn, W.B., Seelig, L.L. & Girard, C.M. Variation in the pattern of carbonic anhydrase activity in the cells of the gastric glands. Histochemistry 39, 289–300 (1974). https://doi.org/10.1007/BF00495680
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00495680