, Volume 59, Issue 3, pp 207–213 | Cite as

ACTH radioimmunocytochemistry (RICH) on rat anterior pituitary cells

  • Gy. Rappay
  • M. Kárteszi
  • G. B. Makara


Radioimmunocytochemistry (RICH) was applied to detect corticotrophs in adult rat pituitaries and 8-day-old anterior pituitary monolayers by incubating sections and cultures with 125I-ACTH-anti ACTH immune complexes. After incubations autoradiography was made. In comparison, “conventional” immunostaining was carried out on adjacent sections and parallel cultures. It has been esteblished that RICH is suitable for detection of corticotrophs.


Public Health Immune Complex Anterior Pituitary Pituitary Cell Adjacent Section 
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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Gy. Rappay
    • 1
  • M. Kárteszi
    • 1
  • G. B. Makara
    • 1
  1. 1.Institute of Experimental MedicineHungarian Academy of SciencesBudapestHungary

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