Advertisement

Histochemistry

, Volume 73, Issue 3, pp 371–375 | Cite as

Incorporation of 3H-befunolol (beta-blocking agent) into melanin granules of ocular tissues in the pigmented rabbits

I. Light microscopic radioautography
  • S. Yamabayashi
  • W. Gunarso
  • S. Tsukahara
  • T. Nagata
Article

Summary

3H-befunolol was administered intravenously to pigmented rabbits. Thirty minutes after the administration, the iris, ciliary body and retina were fixed, embedded and processed for light microscopic radioautography. Radioautographical silver grains were observed over the pigment granules of the iris, ciliary body, choroid and retina. From these results it is concluded that 3H-befunolol is incorporated into the pigment granules of these cells. The mechanism of incorporation is discussed.

Keywords

Public Health Retina Ciliary Body Ocular Tissue Pigment Granule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barbee RF, Smith WO (1957) A comparative study of mydriatic and cycloplegic agents. Am J Ophthalmol 44:617–622Google Scholar
  2. Bénard P, Rico AG, Braun JP, Burgat-Sacazev (1977) New approach to the visualization of receptors by autoradiography. Acta Pharmacol Toxicol 41 (Suppl): 20–21Google Scholar
  3. Bernstein H, Zvaifler N, Rubin M, Mansour AM (1963) The ocular deposition of chloroquine. Invest Ophthalmol 2:384–390Google Scholar
  4. Blois MS (1970) The binding properties of melanin: in vivo and in vitro. Advances in biology of the skin XII. Academic Press, New York, pp 65–79Google Scholar
  5. Chen KK, Poth EJ (1929) Racial difference as illustrated by the mydriatic action of cocaine, euphthalmine and ephedrine. J Pharmacol Exp Ther 36:429–435Google Scholar
  6. Cohen Y (1977) Autoradiographic studies with sympathomimetic amines. Acta Pharmacol Toxicol 61:114–115Google Scholar
  7. Gambill HD, Ogle KN, Kearns TP (1967) Mydriatic effect of four drugs. Arch Ophthalmol 77:740–745Google Scholar
  8. Haddad NJ, Mayer NJ, Riley FC (1970) Mydriatic effect of phenylephrine hydrochloride. Am J Ophthalmol 70:729–734Google Scholar
  9. Hanever WH (1966) Ocular pharmacology. CV Mosby, St. Louis, p 180Google Scholar
  10. Harris LS (1971) Effect of ocular pigmentation on hypotensive response to pilocarpine. Am J Ophthalmol 72:923–925Google Scholar
  11. Hiyama Y (1981) Biochemical analysis on incorporation of 14C-befunolol into melanoma cells in vitro. (in preparation)Google Scholar
  12. Howard HJ, Lee TP (1927) The effect of instillation of ephedrine solution upon the eye. Proc Soc Biol Med 24:700–704Google Scholar
  13. Katz IM, Berger ET (1979) Effect of iris pigmentation on responses of ocular pressure to timolol. Surv Ophthalmol 23:395–398Google Scholar
  14. Kuhn H (1980) Autoradiographic evidence for binding of 3H-flunitrazepan (Rohypnol) to melanin granules in the cat eye. Experientia 36:863–864Google Scholar
  15. Larsson B, Tjälve H (1978) Studies on the melanin affinity of metal ions. Acta Physiol Scand 103:479–484Google Scholar
  16. Luft JH (1977) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–414Google Scholar
  17. Melican HE (1977) Ocular pigmentation and pressure and outflow responses to pilocarpine and epinephrine. Am J Ophthalmol 72:70–73Google Scholar
  18. Nagata T, Shibata O, Nawa T (1967) Simplified methods for mass production of radioautographs. Acta Anat Nippon 42:162–166Google Scholar
  19. Nagata T, Murata F (1977) Electron microscopic dry-mounting radioautography for diffusible compounds by means of ultracryotomy. Histochemistry 54:75–82Google Scholar
  20. Potts AM (1964) The reaction of uveal pigment in vitro with polycyclic compounds. Invest Ophthalmol 3:405–416Google Scholar
  21. Reddy NB (1979) Subcellular distribution and characteristics of beta-adrenergic receptors in human skeletal muscle. Neurology 29:566Google Scholar
  22. Scott JG (1945) The eye of the West African Negro. Br J Ophthalmol 29:12–17Google Scholar
  23. Tsukahara S, Yoshida K, Nagata T (1980) Radioautographical study on the incorporation of 14C-bupranolol (beta-blocking agent) into the rabbit eye. Histochemistry 68:237–244Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • S. Yamabayashi
    • 1
  • W. Gunarso
    • 1
  • S. Tsukahara
    • 1
    • 2
  • T. Nagata
    • 1
  1. 1.Department of AnatomyShinshu University School of MedicineMatsumotoJapan
  2. 2.Department of OphthalmologySuwa Red Cross HospitalSuwaJapan

Personalised recommendations