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Histochemistry

, Volume 73, Issue 3, pp 363–369 | Cite as

Cytochemical localization of photosystem II donor sites

  • K. C. Vaughn
  • St. O. Duke
Article

Summary

Tetramethylbenzidene, an artificial donor of electrons to the photosystem (PS) II reaction center, is oxidized to an osmiophilic polymer that allows for the localization of the donor site of PS II. Mesophyll chloroplasts of Sorghum bicolor (L.) Moench. contain 0.1–0.2 μm deposits only in the grana stacks and on the lumen side of the thylakoid. Agranal mature bundle sheath plastids (known to be devoid of PS II activity) show little deposition and the products appear randomly distributed along the lamellae. The cytochemical localization of PS II donor sites on the lumen side is consistent with flash photolysis and diaminobenzenesulfonic acid inactivation studies as well as the chemiosmotic theory for the generation of a proton gradient.

Keywords

Public Health Polymer Reaction Center Donor Site Proton Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • K. C. Vaughn
    • 1
  • St. O. Duke
    • 1
  1. 1.Southern Weed Science LaboratoryUSDA-SEA-ARStonevilleUSA

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