Summary
The light and electron microscopic study of the lysosomal marker enzymes acid phosphatase (ACPase), β-d-glucuronidase and arylsulphatase parallelly performed on rat livers obtained during a 24-h span yielded the following results:
-
1.
Lysosomal reactions of these three marker enzymes are subjected to significant circadian variations.
-
2.
The lysosome population visualized by these marker enzymes is obviously heterogeneous. The heterogeneity of the lysosomes is expressed by differences in number, size and lobular distribution as well as in phase differences of their maximal and minimal reactivity depending on the marker enzyme used.
-
3.
With each marker enzyme extralysosomal reactions are found and are also subjected to circadian variations of activity.
-
4.
Applying the arylsulphatase reaction, next to the lysosomes patchy areas of strongly reacting endoplasmic reticulum are found. The arylsulphatase reaction of lysosomes and the endoplasmic reticulum is completely inhibited by 0.01 M KCl.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barka T, Anderson PJ (1965) Histochemistry. Hoeber medical division. Harper & Row, New York
Bhattacharya R (1977) Circadian differences of lysosomes and their enzymes. Nova Acta Leopold 46:171–179
Bhattacharya R, Mayersbach H von (1976) Histochemistry of circadian changes of some lysosomal enzymes in rat liver. Acta Histochem (Suppl) 16:109–115
Bitensky L, Chayen J (1977) Histochemical methods for the study of lysosomes. In: Dingle JT (ed) Lysosomes. A laboratory handbook. North-Holland, Amsterdam, pp 207–243
Daems WTh, Brederoo P (1973) Electron microscopical studies on the structure, phagocytic properties, and peroxidatic activity of resident and exudate peritoneal macrophages in the guinea pig. Z Zellforsch 144:247–197
De Duve C, Pressman BC, Gianetto R, Wattiaux R, Appelman F (1955) Tissue fraction studies. 6. Intracellular distribution patterns of enzymes in rat liver tissue. Biochem J 60:604–617
Dodgson KS, Spencer B, Thomas J (1955) Studies on sulphatases. 9. The arylsulphatase of mammalian livers. Biochem J 59:29–37
Dvořák M (1974) Origin and development of lysosomes and peroxisomes. In: Dvořák M (ed) Biogenesis of cell organelles. Purkyne University, Brno, pp 59–86
Geyer G (1973) Ultrahistochemie. Gustav Fischer, Stuttgart
Goldfisher S (1965) The cytochemical demonstration of lysosomal arylsulphatase activity by light and electron microscopy. J Histochem Cytochem 13:520–524
Groh V, Mayersbach H von (1981a) Histochemical tracing of lysosomes-improved preparation technique for acid phosphatase, β-glucuronidase, acid-β-galactosidase and arylesterase in rat liver. Acta Histochem (in press)
Groh V, Mayersbach H von (1981b) Enzymatic and functional heterogeneity of lysosomes. Cell Tissue Res 214:613–621
Hayashi M, Shirahama T, Cohen AS (1968) Combined cytochemical and electron microscopic demonstration of β-glucuronidase activity in rat liver with use of a simultaneous coupling azo dye technique. J Cell Biol 36:289–297
Holt SJ (1958) Indigogenic staining methods for esterases. In: Danielli JF (ed) General cytochemical methods for esterases, Vol 1. Academic Press, New York, pp 375–397
Hopsu-Havu VK, Arstilla AU, Helmien HJ, Kalimo HO (1967) Improvements in the method for electron microscopic localization of arylsulphatase activity. Histochemie 8:54–64
Kuhn D, Schmalzl F, Demmler K (1971) Monozytenleukämie. Dtsch med Wochenschr 96:1594
Lojda Z, Gossrau R, Schiebler TH (1976) Enzymhistochemische Methoden. Springer, Berlin Heidelberg New York
Mayersbach Hv (1974) Circadian liver detoxication and acetylcholinesterase rhythmicity: Two limiting factors in circadian E 600 toxicity. In: Scheving L, Halberg F, Pauly J (eds) Chronobiology. Igakushoin, Tokyo, pp 191–196
Mayersbach Hv, Bhattacharya R (1977) Circumannual variations of liver lysosomes of rats living under constant environmental conditions. Chronobiologia 4:131
Nichols BA, Bainton DF, Farquhar MG (1971) Differentiation of monocytes. Origin, nature and fate of their azurophil granules. J Cell Biol 50:498–515
Pearse AGE (1972) Histochemistry. Theoretical and applied, vol 2. Churchill-Livingstone, Edinburgh London
Pertoft H, Wärmegard B (1978) Heterogeneity of lysosomes originating from rat liver parenchymal cells. Biochem J 174:309–317
Roy AB (1960) The sulphatase of ox liver. The intracellular distribution of sulphatase A and B. Biochem J 77:380–386
Schellens JPM, Daems WTH, Emeiss JJ, Brederoo P, De Bruijn WC, Wisse E (1977) Electron microscopical identification of lysosomes. In: Dingle JT (ed) Lysosomes. A laboratory handbook. North-Holland, Amsterdam, pp 147–208
Uchiyama Y (1980) Chronobiological aspects of cells. Chronological changes in the morphology of hepatocytes. Biomed Res 1:376–391
Uchiyama Y (1981) Circadian alteration in tubular structures on the outer mitochondrial membranes of rat hepatocytes. Cell Tissue Res 21:519–527
Author information
Authors and Affiliations
Additional information
This paper is dedicated to the memory of the late Professor Heinz von Mayersbach
This project was performed at the Medizinische Hochschule Hannover, Federal Republic of Germany, from 1977–1979, during which time Dr. Uchiyama held a fellowship from the Alexander von Humboldt Foundation. The project was supported by a grant from VW-Stiftung
Rights and permissions
About this article
Cite this article
Uchiyama, Y., Groh, V. & von Mayersbach, H. Different circadian variations as an indicator of heterogeneity of liver lysosomes. Histochemistry 73, 321–337 (1981). https://doi.org/10.1007/BF00495647
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00495647