Summary
Prostate glands of adult guinea pigs were stained for nerve growth factor (NGF) and epidermal growth factor (EGF) by immunohistochemical methods. Both NGF and EGF were localized diffusely in the cytoplasm of the glandular epithelial cells, and also in their secretory products. These findings suggest that NGF and EGF are synthesized, stored, and secreted by the glandular epithelial cells of the prostate.
References
Barka T (1980) Biologically active polypeptides in submandibular glands. J Histochem Cytochem 28:836–859
Byyny RL, Orth DN, Cohen S, Doyne ES (1974) Epidermal growth factor: effect of androgens and adrenergic agents. Endocrinology 95:776–782
Carpenter, G, Cohen S (1979) Epidermal growth factor. Annu Rev Biochem 48:193–216
Chapman CA, Banks BEC, Vernon CA, Walker JM (1981) The isolation and characterisation of nerve growth factor from the prostate gland of the guinea-pig. Eur J Biochem 115:347–351
Cohen S (1962) Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the newborn animal. J Biol Chem 237:1555–1562
Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14, 291–302
Harper GP, Barde YA, Burnstock G, Carstairs JR, Dennison ME, Suda K, Vernon CA (1979) Guinea pig prostate is a rich source of nerve growth factor. Nature 279:160–162
Harper GP, Glanville RW, Thoenen H (1982) The purification of nerve growth factor from bovine seminal plasma: biochemical characterization and partial amino acid sequence. J Biol Chem 257:8541–8548
Harper GP, Thoenen H (1980) The distribution of nerve growth factor in the male sex organs of mammals. J Neurochem 34:893–903
Levi-Montalcini R, Angeletti PU (1968) Nerve growth factor. Physiol Rev 48:534–569
Nakane PK, Kawaoi A (1974) Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem 22:1084–1091
Thoenen H, Barde YA (1980) Physiology of nerve growth factor. Physiol Rev 60:1284–1335
Wallace LT, Partlow LM (1976) α-Adrenergic regulation of secretion of mouse saliva rich in nerve growth factor. Proc Natl Acad Sci USA 73:4210–4214
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shikata, H., Utsumi, N., Hiramatsu, M. et al. Immunohistochemical localization of nerve growth factor and epidermal growth factor in guinea pig prostate gland. Histochemistry 80, 411–413 (1984). https://doi.org/10.1007/BF00495427
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00495427