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Über hydrophobe Acridinfarbstoffe zur Fluorochromierung von Mitochondrien in lebenden Zellen

Hydrophobic acridine dyes for fluorescence staining of mitochondria in living cells. 2. Comparison of staining of living and fixed HeLa-cells with NAO and DPPAO

2. Mitteilung: Vergleich der Färbung lebender und fixierter HeLa-Zellen mit NAO und DPPAO

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Summary

The hydrophobic fluorescence dyes NAO and DPPAO (see scheme of structural formulae) stain the mitochondria of living HeLa-cells. The trans-membrane potential favours the dye accumulation of the cation NAO and supports the hydrophobic interaction of the dye with the mitochondrial membrane lipids and proteins. The lecithinlike dye DPPAO is electrical neutral. Its binding to mitochondria of living cells is only caused by hydrophobic interaction. NAO and DPPAO stain also the mitochondria of glutaraldehyde fixed HeLa-cells in aqueous medium. Fluorescence staining occures even after extraction of the lipids of the cell with acetone. We suppose that the dye accumulation in the mitochondria of the fixed cells is caused by the hydrophobic interaction between the dyes and the very hydrophobic mitochondrial lipids and proteins.

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Authors and Affiliations

  1. Institut für Physikalische Chemie der Universität Freiburg i. Br., Albertstraße 21, D-7800, Freiburg, Federal Republic of Germany

    U. Erbrich, M. Septinus, A. Naujok & H. W. Zimmermann

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  1. U. Erbrich
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  2. M. Septinus
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  3. A. Naujok
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  4. H. W. Zimmermann
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Erbrich, U., Septinus, M., Naujok, A. et al. Über hydrophobe Acridinfarbstoffe zur Fluorochromierung von Mitochondrien in lebenden Zellen. Histochemistry 80, 385–388 (1984). https://doi.org/10.1007/BF00495422

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  • DOI: https://doi.org/10.1007/BF00495422

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