Skip to main content
SpringerLink
Log in

Enzyme-and immuno-histochemical localization of kallikrein

I. The human parotid gland

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

Localization of kallikrein in the human parotid gland was investigated simultaneously by two markers: kallikrein-like (enzyme) activity and kallikrein antigenicity. Kallikrein-like activity was histochemically demonstrated by using a synthetic substrate, pro-phe-arg-naphthylester. Kallikrein antigenicity was demonstrated by an unlabelled antibody peroxidase-antiperoxidase method, where monospecific antiserum against highly purified urinary kallikrein was used as the primary antiserum. The results showed that kallikrein-like activity and kallikrein antigenicity were identical in their locations in the ductal cells, being localized in the luminal part of the striated ducts and to a lesser degree in the excretory ducts. This indicates the presence of active kallikrein in these regions. No enzyme activity nor antigenicity was observed either in acini or in intercalated ducts. Moreover, the peroxidase-antiperoxidase method reveated kallikrein antigenicity for the first time extracellularly in the basement-membrane region of acini and of ducts as well as in the interstitium surrounding ducts and major vessels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Albrechtsen R, Wewer U, Wimberley PD (1980) Immunohistochemical demonstration of a hitherto undescribed localization of hemogloblin A and F in endodermal cells of normal human yolk sac and endodermal sinus tumor. Acta Pathol Microbiol Scand Sect A 88:175–178

    Google Scholar 

  • Albrechtsen R, Nielsen M, Wewer U, Engvall E, Ruoslahti E (1981) Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin. Cancer Res 41:5076–5081

    Google Scholar 

  • Arnold WH, Ørstavik TB, Holck M (1983) A 4-methoxy-2-naphthylamide substrate for the histochemical localization of esteroproteases in the submandibular gland of the rat. Histochem J 15:139–146

    Google Scholar 

  • Brandtzaeg P, Gautvik KM, Nustad K, Pierce JV (1976) Rat submandibular gland kallikreins: purification and cellular localization. Br J Pharmacol 56: 155–167

    Google Scholar 

  • Curran RC, Gregory J (1977) The unmasking of antigens in paraffin sections of tissue by trypsin. Experientia 33:1400–1401

    Google Scholar 

  • Corthorn J, Imanari T, Yoshida T, Kaizu T, Pierce JV, Pisano JJ (1979) Isolation of prokallikrein from human urine. In: Fujii S, Mariya H, Suzuki T (eds) Kinin II. Systemic proteases and cellular function. Plenum Press, New York London, pp 575–579

    Google Scholar 

  • Dietl T, Kruck J, Fritz H (1978) Localization of kallikrein in porcine pancreas and submancibular gland as revealed by the indirect immunofluorescence technique. Hoppe-Seyler's Z Physiol Chem 359:499–505

    Google Scholar 

  • Dixon M, Webb EC (1979) Enzymes. 3rd ed. Longman Group Ltd, London, pp 274–276

    Google Scholar 

  • Fiedler F (1979) Enzymology of glandular kallikreins. In: Erdös EG (ed) Handbook of experimental pharmacology Vol 25 Suppl: Bradykinin, kallidin and kallikrein. Springer, Berlin Heidelberg New York, pp 104–161

    Google Scholar 

  • Fritz H, Fiedler F, Dietl T, Warwas M, Truscheit E, Kolb J, Mair G Tschesche H (1977) On the relationships between porcine pancreatic, submandibular and urinary kallikreins. In: Haberland GL, Rohen JW, Suzuki T (eds) Kininogenases 4. Kallikrein-physiological properties and pharmacological rationals. Schattauer, Stuttgart New York, pp 15–28

    Google Scholar 

  • Garrett JR, Smith RE, Kidd A, Kyriacou K (1981) A new microscopical method for demonstrating kallikrein-like activity. J Microse 125:RP1-RP2

    Google Scholar 

  • Garrett JR, Smith RE, Kidd A, Kyriacou K, Grabske RJ (1982) Kallikrein-like activity in salivary glands using a new tripeptide substrate, including preliminary studies and observations on mast cells. Histochem J 14: 967–979

    Google Scholar 

  • Hilton SM (1970) The physiological role of glandular kallikreins. In: Erdös EG (ed) Handbook of experimental pharmacology. Vol 25: Bradykinin, kallidin and kallikrein. Springer, Berlin Heidelberg New York, pp 389–399

    Google Scholar 

  • Hojima Y, Maranda B, Moriwaki C, Schachter M (1977) Direct evidence for the location of kallikrein in the striated ducts of the cat's submandibular gland by the use of specific antibody. J Physiol 268: 793–801

    Google Scholar 

  • Kimura K, Takagi M, Igari T, Ishii, M, Ikeda T, Takeda T, Murao S (1982) Histochemical localization of kallikrein-like pro-phearg-naphthylester esterase activity in the rat kidney. Histochemistry 75: 91–98

    Google Scholar 

  • Kimura K (1983) Segmental localization of kallikrein-like pro-phearg-naphthylester esterase in the rat nephron. Acta Pathol Microbiol Immunol Scand Sect A 91: 35–42

    Google Scholar 

  • Miyaura S, Matsuda Y, Yamaguchi K, Moriya H (1981) Detection and elimination of serum protein contaminants during the purification of human urinary kallikrein. Chem Pharm Bull 29: 855–860

    Google Scholar 

  • Rabito SF, Scicli AG, Kher V, Carretero OA (1982) Immunoreactive glandular kallikrein in rat plasma: a radioimmunoassay for its determination. Am J Physiol 242: H602-H610

    Google Scholar 

  • Schachter M, Maranda B, Moriwaki C (1978) Localization of kallikrein in the coagulating and submandibular glands of the guinea pig. J Histochem Cytochem 26: 318–321

    Google Scholar 

  • Schachter M, Peret MW, Moriwaki C, Rodrigues JAA (1980) Localization of kallikrein in submandibular gland of cat, guinea pig, dog and man by the immunoperoxidase method. J Histochem Cytochem 28: 1295–1300

    Google Scholar 

  • Simson JAV, Spicer SS, Chao J, Grimm L, Margolius HS (1979) Kallikrein localization in rodent salivary glands and kidney with the immunoglobulin-enzyme bridge technique. J Histochem Cytochem 27: 1567–1576

    Google Scholar 

  • Sternberger LA (1979) Immunocytochemistry, 2nd ed. John Wiley & Sons, New York, pp 104–130

    Google Scholar 

  • Uddin M, Tyler D (1981) The localization of kallikrein in the dog and guinea-pig submandibular glands. Experientia 37: 872–878

    Google Scholar 

  • Ørstavik TB, Brandtzaeg P, Nustad K, Halvorsen KM (1975) Cellular localization of kallikreins in rat submandibular and sublingual salivary glands. Acta Histochem 54: 183–192

    Google Scholar 

  • Ørstavik TB, Gautvik KM, Nustad K, Hedemark-Poulsen J (1978) Diffusion of 125I-labeled glandular kallikrein after local administration in the rat submandibular gland. Microvasc Res 15: 115

    Google Scholar 

  • Ørstavik TB, Brandtzaeg P, Nustad K, Pierce JV (1980) Immunohistochemical localization of kallikrein in human pancreas and salivary glands. J Histochem Cytochem 28: 557–562

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. University Institute of Pathology, University of Copenhagen, 11, Frederik V's Vej, DK-2100, Copenhagen, Dennark

    K. Kimura & H. Moriya

  2. Faculty of Pharmaceutical Sciences, Science University of Tokyo, Ichigaya Funagawaramachi 12, Shinjuku-ku, Tokyo, Japan

    K. Kimura & H. Moriya

Authors
  1. K. Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Moriya
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kimura, K., Moriya, H. Enzyme-and immuno-histochemical localization of kallikrein. Histochemistry 80, 367–372 (1984). https://doi.org/10.1007/BF00495419

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00495419

Keywords

Search

Navigation