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Histochemistry

, Volume 80, Issue 4, pp 347–351 | Cite as

Microphotometric determination of enzyme activities in type-grouped fibres of reinnervated rat muscle

  • C. Vetter
  • H. Reichmann
  • D. Pette
Article

Summary

Activities of succinate dehydrogenase (SDH), glycerolphosphate oxidase (GP-OX), cytochrome oxidase (CYT-OX) and lactate dehydrogenase (LDH) were determined microphotometrically in single, actomyosin-ATPase typed (Guth and Samaha 1970) fibres within cross-sections of normal and reinnervated rat tibialis anterior muscles. SDH and GP-OX activities displayed pronounced scattering and large overlaps existed between α-, αβ-, and β-fibres of normal muscle. Coefficients of variation were in the range of 16–40% for GP-OX and SDH in the different fibre populations. Enzyme activity determinations in typegrouped α-, αβ-, and β-fibres of reinnervated muscle showed much less scattering than in normally distributed α-, αβ-, and β-fibres of control muscles. Coefficients of variation were in the range of 10–13% for SDH, GP-OX, CYT-OX and LDH. The experimental error of the kinetic microphotometric measurement of enzyme activities in situ is in the range of 10% (Reichmann and Pette 1982). Our results therefore suggest a high degree of metabolic similarity or homogeneity of typed-grouped muscle fibres and thus support the assumption that type-groped fibres are homogeneous and correspond to regularly assembled motor units.

Keywords

Succinate Lactate Dehydrogenase Motor Unit Cytochrome Oxidase Succinate Dehydrogenase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • C. Vetter
    • 1
  • H. Reichmann
    • 1
  • D. Pette
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzFederal Republic of Germany

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