, Volume 81, Issue 1, pp 67–73 | Cite as

Inter- and intraspecies comparisons of fibre type distribution and of succinate dehydrogenase activity in type I, IIA and IIB fibres of mammalian diaphragms

  • H. J. Green
  • H. Reichmann
  • D. Pette


Fibre types in the costal region of the diaphragm muscle of several mammalian species with widely different respiratory rates were examined microphotometrically for succinate dehydrogenase (SDH) activity. Mean activities indicated no significant (p>0.05) difference between the type I and IIA fibres for any of the species examined. SDH activities in type IIB fibres were significantly lower (p<0.05) than either the type I or type IIA fibres in the cat, guinea pig, rat and rabbit whereas in the mouse no difference was found. The dog had no classical type 1B fibres. Analysis of the distribution of SDH activities by fibre type indicated a wide scattering of scores with no distinct separation between fibre types. Large differences in SDH activity were noted between species. Mean SDH activities were highest in the mouse and rat, intermediate in the rabbit and guinea pig and lowest in the cat and dog. These data suggest an association between respiratory rate and aerobic oxidative potential of the various fibre types in diaphragms of the species examined.


Fibre Type Succinate Dehydrogenase Diaphragm Muscle Costal Region Succinate Dehydrogenase Activity 
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  1. Barany M (1967) ATPase activity of myosin correlated with speed of muscle shortening. J Gen Physiol 50:197–218Google Scholar
  2. Bass A, Gutmann E, Hanikova M (1971) Contraction properties and enzyme properties of the mouse and rat diaphragm. Physiol Bohemoslov 20:5–9Google Scholar
  3. Brooke MH, Kaiser KK (1970) Three “myosin ATPase” systems: the nature of their pH lability and sulphydryl dependence. J Histochem Cytochem 18:670–672Google Scholar
  4. Crosfill ML, Widdicombe JG (1961) Physical characteristics of the chest and lungs and the work of breathing in different mammalian species. J Physiol (Lond) 158:1–14Google Scholar
  5. Davies AS, Gunn HM (1972) Histochemical fibre types in mammalian diaphragm. J Anat 112:41–60Google Scholar
  6. Essén B, Jansson E, Henriksson J, Taylor AW, Saltin B (1975) Metabolic characteristics of fibre types in human skeletal muscle. Acta Physiol Scand 95:153–165Google Scholar
  7. Faulkner JA, Maxwell LC, Ruff GL, White TP (1979) The diaphragm as a muscle. Am Rev Respir Dis 119:89–92Google Scholar
  8. Faulkner JA, Jones DA, Round JM, Edwards RWT (1980) Dynamics of energetic processes in human muscle. In: Ceretello P, Whipp BJ (eds) Exercise bioenergetics and gas exchange. Elsevier/North Holland Biomedical Press, Amsterdam, pp 81–90Google Scholar
  9. Gauthier GF, Padykula HA (1966) Cytological studies of fiber types in skeletal muscle. A comparative study of the mammalian diaphragm. J Cell Biol 28:333–354Google Scholar
  10. Gottschall J (1981) The diaphragm of the rat and its innervation. Muscle fiber composition; perikarya and axons of efferent and afferent neurons. Anat Embryol 161:405–417Google Scholar
  11. Green HJ, Reichmann H, Pette D (1982) A comparison of two ATPase based schemes for histochemical muscle fibre typing in various mammals. Histochemistry 76:21–31Google Scholar
  12. Gunn HM (1978) Differences in the histochemical properties of skeletal muscles of different breeds of horses and dogs. J Anat 127:615–634Google Scholar
  13. Hoppeler H, Mathieu O, Krauer R, Claassen H, Armstrong R, Weibel E (1981) Design of the mammalian respiratory system. VI. Distribution of mitochondria and capillaries in various tissues. Respir Physiol 44:87–111Google Scholar
  14. Keens TG, Chen V, Patel P, O'Brien P, Levison H, Ianuzzo CD (1978) Cellular adaptations of the ventilatory muscles to a chronic increased respiratory load. J Appl Physiol 44:905–908Google Scholar
  15. Lieberman DA, Faulkner JA, Craig AB Jr, Maxwell LC (1973) Performance and histochemical composition of guinea pig and human diaphragm. J Appl Physiol 34:233–237Google Scholar
  16. Nemeth P, Pette D (1981) Succinate dehydrogenase activity in fibres classified by myosin ATPase in three hind limb muscles of the rat. J Physiol (Lond) 320:73–80Google Scholar
  17. Nemeth P, Hofer HW, Pette D (1979) Metabolic heterogeneity of muscle fibres classified by myosin ATPase. Histochemistry 63:191–201Google Scholar
  18. Peter JB, Barnard RJ, Edgerton VR, Gillespie CA, Stempel KE (1972) Metabolic profiles of three fibre types of skeletal muscle in guinea pigs and rabbits. Biochemistry 11:2627–2634Google Scholar
  19. Pette D, Tyler KR (1983) Response of succinate dehydrogenase activity in fibres of rabbit tibialis anterior muscle to chronic nerve stimulation. J Physiol (Lond) 338:1–9Google Scholar
  20. Pette D, Wasmund H, Wimmer M (1979) Principle and method of kinetic microphotometric enzyme activity determination in situ. Histochemistry 64:1–10Google Scholar
  21. Reichmann H, Pette D (1982) A comparative microphotometric study of succinate dehydrogenase activity levels in type I, IIA and IIB fibres of mammalian and human muscles. Histochemistry 74:27–41Google Scholar
  22. Reichmann H, Pette D (1984) Glycerolphosphate oxidase and succinate dehydrogenase activities in IIA and IIB fibres of mouse and rabbit tibialis anterior muscles. Histochemistry 80:429–433Google Scholar
  23. Riley DA, Berger AJ (1979) A regional histochemical and electromyographic analysis of the cat respiratory diaphragm. Exp Neurol 66:636–649Google Scholar
  24. Salmons S, Henriksson J (1981) The adaptive response of skeletal muscle to increased use. Muscle Nerve 4:94–105Google Scholar
  25. Sieck GC, Roy RR, Powell P, Blanco C, Edgerton VR, Harper RM (1983) Muscle fiber type distribution and architecture of the cat diaphragm. J Appl Physiol 55:1386–1392Google Scholar
  26. Yellin H (1972) Differences in histochemical attributes between diaphragm and hind leg muscles of the rat. Anat Rec 173:333–340Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • H. J. Green
    • 1
  • H. Reichmann
    • 1
  • D. Pette
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzFederal Republic of Germany

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