Histochemistry

, Volume 58, Issue 4, pp 297–305 | Cite as

Electroncytochemical and biochemical demonstration of guanylate cyclase activity in the pancreatic islet

  • J. S. T. Tse
  • Y. Shibayama
  • B. J. Lin
Article

Summary

With a cytochemical method using guanylyl imidodiphosphate as a substrate, the guanylate cyclase activity was localized on the plasma membrane of A, B and D cells of islets of Langerhans isolated from the rat. Adequate control experiments were performed by a double-blind method. Parallel biochemical assay showed that guanylate cyclase activity was not completely lost after fixation with 1% glutaraldehyde and incubation with 4 mM lead nitrate. Furthermore, the depressed activity was still stimulatable with acetylcholine.

Keywords

Public Health Nitrate Plasma Membrane Acetylcholine Control Experiment 

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • J. S. T. Tse
    • 1
  • Y. Shibayama
    • 1
  • B. J. Lin
    • 1
  1. 1.Department of Physiology, Faculty of MedicineUniversity of TorontoTorontoCanada

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