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Histochemistry

, Volume 58, Issue 4, pp 281–288 | Cite as

Quantitative determination of G6Pase activity in histochemically defined zones of the liver acinus

  • H. F. Teutsch
Article

Summary

Qualitative histochemical G6Pase distribution patterns obtained with an improved method (Teutsch, 1978) served as the basis for a zonal microdissection of the liver acinus. G6Pase activity was determined quantitatively in tissue samples of zones 1 and 3 by a microfluorometric method (Burch et al., 1978). Using a correlation system it could be demonstrated that the histochemical distribution pattern obtained with the improved method was in better agreement with quantitatively estimated zonal differences of G6Pase activity, both in fed and starved female rats, than with the Wachstein and Meisel medium (1956). From a total of 50 tissue samples analyzed the following average G6Pase activities were calculated: in fed animals 15.36±3.48 U/g dry weight in zone 1, and 9.28±2.15 U/g dry weight in zone 3; in starved female rats 42.50±8.20 U/g dry weight in zone 1, and 29.25±5.68 U/g dry weight in zone 3. The qualitative histochemical as well as quantitative zonal differences of G6Pase activities are taken as further support for the hypothesis of metabolic zonation of liver parencyma.

Keywords

Public Health Tissue Sample Distribution Pattern Quantitative Determination Correlation System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Burch, H.B., Narins, R.G., Chu, C., Fagioli, S., Choi, S., McCarthy, W., Lowry, O.H.: Distribution along the rat nephron of three enzymes of gluconeogenesis in acidosis and starvation. Am. J. Physiol. 235, in press (1978)Google Scholar
  2. Brauss, E., Sasse, D.: Die Aktivität des glykogensynthetisierenden Enzyms (UDPGGT) in der Leber unter normalen und experimentellen Bedingungen. Histochemie 14, 260–269 (1968)Google Scholar
  3. Chiquoine, A.D.: The distribution of glucose-6-phosphatase in the liver and kidney of the mouse. J. Histochem. Cytochem. 1, 429–439 (1953)Google Scholar
  4. Chiquoine, A.D.: Further studies on the histochemistry of glucose-6-phosphatase. J. Histochem. Cytochem. 3, 471–478 (1955)Google Scholar
  5. Katz, N., Teutsch, H.F., Sasse, D., Jungermann, K.: Heterogeneous distribution of glucose-6-phosphatase in microdissected periportal and perivenous rat liver tissue. FEBS Lett. 76, 226–230 (1977)Google Scholar
  6. Lowry, O.H., Passonneau, J.V.: A flexible system of enzymatic analysis. New York, San Francisco, London: Academic Press 1972Google Scholar
  7. Morrison, G.R., Brock, F.E., Karl, I.E., Shank, R.E.: Quantitative analysis of regenerating and degenerating areas within the lobule of the carbon tetrachloride-injured liver. Arch. Biochem. Biophys. 111, 448–460 (1965)Google Scholar
  8. Rappaport, A.M.: The microcirculatory acinar concept of normal and pathological hepatic structure. Beitr. Path. 157, 215–243 (1976)Google Scholar
  9. Rieder, H., Teutsch, H.F., Sasse, D.: NADP-dependent dehydrogenases in rat liver parenchyma. I. Methodological studies on the qualitative histochemistry of G6PDH, 6PGDH, malic enzyme and ICDH. Histochemistry 57, 283–298 (1978).Google Scholar
  10. Sasse, D., Köhler, K.: Die topochemische Verlagerung von Funktionseinheiten des Glykogenstoffwechsels in der Leber durch Allylformiat. Histochemie 18, 325–336 (1969)Google Scholar
  11. Sasse, D., Schenk, A.: Räumliche Darstellung der funtionellen Lebereinheit (Azinus). Acta anat. 93, 78–87 (1975)Google Scholar
  12. Sasse, D., Katz, N., Jungermann, K.: Functional heterogeneity of rat liver parenchyma and of isolated hepatocytes. FEBS Lett. 57, 83–88 (1975)Google Scholar
  13. Sasse, D.: Dynamics of liver glycogen. The topochemistry of glycogen synthesis, glycogen content and glycogenolysis under the experimental conditions of glycogen accumulation and depletion. Histochemistry 45, 237–254 (1975)Google Scholar
  14. Schmidt, U., Schmid, H., Guder, W.G.: Liver cell heterogeneity. The distribution of fructosebisphosphatase in fasted rats and in man. Hoppe-Seyler's Z. Physiol. Chem. 359, 193–198 (1978)Google Scholar
  15. Suzuki, K.: Quantitative enzyme histochemistry of normal and injured liver part 2; ultramicrochemical determination of NADP-dependent isocitrate and glucose-6-phosphate dehydrogenase in the lobules of normal and injured human liver. Acta Histochem. Cytochem. 9, 30–38 (1976)Google Scholar
  16. Teutsch, H.F., Sasse, D., Katz, N., Jungermann, K.: Zur qualitativen und quantitativen Chemomorphologie des Leberparenchyms (G6Pase). Verh. Anat. Ges. 72, 665–667 (1978)Google Scholar
  17. Teutsch, H.F.: Improved method for the histochemical demonstration of glucose-6-phosphatase activity. A methodological study. Histochemistry 56, 107–117 (1978)Google Scholar
  18. Thaler, M.E., Kissane, J.M.: Quantitative distribution of lactic dehydrogenase in the regenerating liver lobule of the rat. J. Histochem. Cytochem. 14, 495–500 (1966)Google Scholar
  19. Wachstein, M., Meisel, E.: On the histochemical demonstration of glucose-6-phosphatase. J. Histochem. Cytochem. 4, 592 (1956)Google Scholar
  20. Wimhurst, J.M., Manchester, K.L.: A comparison of the effects of diabetes induced with either alloxan or streptozotozin and of starvation on the activities in rat liver of the key enzymes of gluconeogenesis. Biochem. J. 120, 95–103 (1970)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • H. F. Teutsch
    • 1
  1. 1.Lehrstuhl Anatomie IIIAnatomisches Institut der Universität FreiburgFreiburg i.Br.Germany

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