Summary
Cytochemical changes in the metathoracic dorsal longitudinal flight muscle of the female desert locust, Schistocerca gregaria, during its differentiation from the last day of the fourth larval instar, through the fifth larval instar up to the eighth day after the imaginal moult were examined with respect to: (1) DNA content, (2) transcriptioanl activity, (3) degree of chromatin condensation, (4) RNA content, (5) protein content and (6) cross-sectional fibre area.
Microspectrophotometric analysis of Feulgen-stained nuclei shows that polyploidization is not a phenomenon associated with the differentiation of these muscles. The nuclei remain diploid throughout the developmental period.
By “staining” with 3H-Actinomycin D, followed by autoradiography, the changes in transcriptional activity coincident with flight muscle development were examined. At the beginning of the developmental period the transcriptional activity is relatively high and remains so up until about the middle of the fifth instar after which time it declines fairly steadily. These changes in transcriptional activity are accompanied by changes in the degree of chromatin condensation. Generally, the periods of high transcriptional activity exhibit less condensation of the chromatin.
The originally high transcriptional activity is accompanied by a steady over-all increase in total RNA content during the developmental period; and in turn a large increase in total protein content. The cross-sectional area of the muscle fibres increases significantly during the growth and differentiation of the flight muscles.
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References
Badr, E. A.: Cytophotometric studies on histones in different types of cells as compared to the degree of binding of 3H-actinomycin D to their nuclei. Arch. Biol. (Liege) 83, 21–27 (1972)
Bartels, P. H., Bahr, G., Jeter, W., Olson, G., Taylor, J., Weid, G.: Evaluation of correlational information in digitized cell images. J. Histochem. Cytochem. 22, 69–79 (1974)
Basleer, R.: Etude l'augmentation du nombre de noyaux dans des bourgeions musculaired cultives in vitro. Z. Anat. Entwickl.-Gesch. 123, 184–205 (1962)
Beenakkers, A. M.: Enzyme der dettsaure-oxydation in den Flugmuskeln von Locusta migratoria während ihrer Entwicklung. Biochem. Z. 337, 436–439 (1963)
Beenakkers, A. M.: Regulation of the development of catabolic properties in insect flight muscles. XXVI. International Congress of Physiological Sciences (Abstract) (1974)
Brachet, J., Ficq, A.: Binding sites of 14C-actinomycin D in amphibian ovocytes and an autoradiography technique for the detection of cytoplasmic DNA. Exp. Cell Res. 38, 152–159 (1965)
Brachet, J., Hulin, N.: Binding of tritiated actinomycin and cell differentiation. Nature (Lond.) 222, 481–482 (1969)
Brachet, J., Malpoix, P.: Macromolecular synthesis and nucleocytoplasmic interactions in early development. In: Adv. Morphogenesis vol. 9, (M. Abercombie and J. Brachet, eds.) p. 263–316. New York and London: Academic Press 1971
Brosemer, R. W., Vogell, W., Bücher, Th.: Morphologische und enzymatische Muster bei der Entwicklung indirekter Flugmuskeln von Locusta migratoria. Biochem. Z. 338, 854–910 (1963)
Bücher, Th.: Formation of the specific structural and enzymic pattern of the insect flight muscle. In: Aspects of Insect Biochemistry, (T. W. Goodwin ed.) p. 15–28. London-New York: Academic Press 1965
Burkhardt, H.: Analysis of variance-PDP-5/8. Decus Program Library, Decus No. 5/8-9 (1964)
Cox, P. C., Simpson, S. B.: A microphotometric study of myogenic lizard cells grown in vitro. Develop. Biol. 23, 433–443 (1970)
Chrambach, A., Reisfeld, R. A., Wychoff, M., Zaccari, J.: A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. Analyt. Biochem. 20, 150–154 (1967)
Darzcynkiewicz, Z., Geldhill, B. D., Ringertz, N. R.: Changes in deoxyribonucleoprotein during spermiogenesis in the bull. Exp. Cell Res. 58, 435–438 (1969)
Deitch, A. D.: Cytophotometry of nucleic acids. In: Introduction to Quantitative Cytochemistry (G. L. Weid, ed.), New York and London: Academic Press 1966
Fand, S.: Environment conditions for optimal Feulgen hydrolysis. In: Introduction to Quantitative Cytochemistry-II, (G. L. Weid and G. F. Bahr, eds.), p. 211–222. New York and London: Academic Press 1972
Fazekas de St. Groth, S., Webster, R. G., Datyner, A.: Two new staining procedures for quantitative estimation of proteins on electrophoretic strips. Biochim. biophys. Acta (Amst.) 71, 377–391 (1963)
Firket, H.: Researches sur la synthese des acides, desocyribonucleiques et la preparation a la mitose dans des cellules cultivees in vitro. Arch. Biol. (Liége) 69, 1–166 (1958)
Fischman, D. E.: Development of striated muscle. In: The Structure and Function of Muscle, vol. 1, (G. H. Bourne ed.) p. 75–148. New York and Londen: Academic Press 1972
Flax, M. H., Himes, M. H.: Microspectrophotometric analysis of metachromatic staining of nucleic acids. Physiol. Zool. 25, 297–311 (1952)
Fox, D. P.: DNA values in somatic tissues of Dermestes (Dermistidae: Coleoptera) I. Abdominal fat body and testis wall of the adult. Chromosoma (Berl.) 28, 445–456 (1969)
Garcia, A. M.: Stoichimetry of Dye Binding versus Degree of Chromatin Coiling. In: Introduction to Quantitative Cytochemistry-II, (G. L. Weid and G. F. Bahr, eds.), p. 153–170. New York-London: Academic Press 1970
Goldspink, G.: Postembryonic growth and differentiation of striated muscle. p. 179–236. In: The Structure and Function of Muscle, vol. 1, (G. H. Bourne, ed.), p. 179–236. New York-London: Academic Press 1972
Hill, L., Goldsworthy, G. J.: Growth, feeding activity and the utilization of reserves in larvae of Locusta. J. Insect Physiol. 14, 1085–1098 (1968)
Hill, L., Luntz, A. J., Steele, P. A.: The relationships between somatic growth, ovarian growth, and feeding activity in the adult desert locust. J. Insect Physiol. 14, 1–20 (1968)
Holtzer, H., Weintraub, H., Mayne, R., Mochan, B.: The cell cycle, cell lineages and cell differentiation. In: Current Topics in Developmental Biology, Vol. 7, (A. A. Moscona and A. Monroy eds.), p. 229–259. New York-London: Academic Press 1972
Hunter-Jones, P.: Instructions for Rearing and Breeding Locusts in the Laboratory. London: Anti-Locust Research Centre 1956
Keifer, R., Keifer, G., Salm, R., Rossner, R., Sandritter, W.: A method for the quantitative evaluation of eu- and heterochromatin in interphase nuclei using cytophotometry and pattern analysis. Beitr. Path. 150, 163–173 (1973)
Kleinow, W., Sebald W., Neupert, W., Büchner, Th.: Formation of mitochondria of Locusta migratoria flight muscles. In: Autonomy and Biogenesis of Mitochondria and chloroplasts. (Boardman, N. K., Linnane, A. W., Smillie, R. M., eds.), p. 140–151. Amsterdam: North-Holland Publ. Comp. 1970
Lash, J. W., Holtzer, H., Swift, H.: Regeneration of mature skeletal muscle. Anat. Rec. 128, 679–693 (1957)
Mahon, D. C., Nair, K. K.: Stoichiometry of aldehyde fuchsin staining reaction for proteins. J. Histochem. Cytochem. (in press 1975)
Mittwoch, U., Kalmus, H., Webster, W.: Deoxyribonucleic acid values in dividing and nondividing cells of male and female larvae of the honey bee. Nature (Lond.) 210, 264–266 (1966)
Moss, F., Leblond, C. P.: Nature of dividing nuclei in skeletal muscle of growing rats. J. Cell. Biol. 44, 459–462 (1970)
Nair, K. K., Menon, M.: Detection of juvenile-hormone-induced gene activity in the colleterial gland nuclei of Periplaneta by actinomycin D ‘staining’ technique. Experienta (Basel) 28, 577 (1972)
Richard, C., Tanguay, R., Chaudhary, K. D.: Nucleic acid and protein variations in relation to ultrastructural changes in the developing thoracic muscles of the African desert locust, Schistocerca gregaria. Cytobios 10–11, 145–160 (1971)
Rigler, R., Killander, D., Bolund, L., Ringertz, N. R.: Cytochemical characterization of deoxyribonucleoprotein in individual cell nuclei. Exp. Cell Res. 55, 215–224 (1969)
Ringertz, N. R., Bolund, L.: Actinomycin binding capacity of deoxyribonucleoprotein. Biochim. biophys. Acta (Amst.) 174, 147–154 (1969a)
Ringertz, N. R., Bolund, L.: “Activation” of hen erythrocyte deoxyribonucleoprotein. Exp. Cell Res. 55, 205–214 (1969b)
Ringertz, N. R., Darzcynkeiwicz, Z., Bolund, L.: Actinomycin binding properties of stimulated human lymphocytes. Exp. Cell Res. 56, 411–417 (1969)
Tiegs, O. W.: The flight muscles of insects—their anatomy and histology; with some observations on the structure of striated muscle in general. Phil. Trans. B 238, 221–359 (1955)
Vogell, W.: Phasen der Bildung morphologischer und enzymatischer Muster der Flugmuskel der Wanderheuschrecke. Naturwissenschaften 52, 405–418 (1965)
Walker, P. R., Hill, L., Bailey, E.: Feeding activity, respiration and lipid and carbohydrate content of the male desert locust during adult development. J. Insect Physiol 16, 1001–1015 (1970)
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Panar, L.C., Nair, K.K. Cytochemistry of the differentiating flight muscles of the desert locust Schistocerca gregaria . Histochemistry 45, 129–141 (1975). https://doi.org/10.1007/BF00495157
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DOI: https://doi.org/10.1007/BF00495157