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Multiple localizations of adenylate cyclase in rat hippocampus

A histochemical study

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Summary

A new method for the histochemical demonstration of adenylate cyclase activity, introduced and biochemically tested by Poeggel et al. (1981a), was employed in nervous tissue. Using this method a multiple pattern of activity was detectable. Activity occurs in nervous as well as glial elements. Biochemical results and physiological conclusions could be confirmed by ultrahistochemical visualization of adenylate cyclase activity in nervous tissue. The specificity of the reaction is controlled by a number of variations of the incubation methods.

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References

  • Blosser JC, Appel SH (1980) Regulation of acetylcholine receptor by cyclic AMP. J Biol Chem 255:1235–1238

    Google Scholar 

  • Bray JJ, Kon CM, Breckenridge BMcL (1971) Adenyl cyclase, cyclic nucleotide phosphodiesterase and axoplasmic flow. Brain Res 26:385–394

    Google Scholar 

  • Butcher RW, Ho RJ, Meng HC, Sutherland EW (1965) Adenosine 3′,5′-monophosphate in biological materials: II. The measurement of cAMP in tissue and the role of the cyclic nucleotide in the lipolytic response of fat to epinephrine. J Biol Chem 240:4515–4523

    Google Scholar 

  • Carnegie PR, Durkley PR, Kemp BE, Murray AW (1974) Phosphorylation of selected serine and threonine residues in myelin basic protein by endogenous and exogenous protein kinases. Nature 249:147–150

    Google Scholar 

  • Cheng H, Farquhar MG (1976) Presence of adenylate cyclase activity in Golgi and other fractions from rat liver. Biochemical determination. J Cell Biol 70:660–670

    Google Scholar 

  • Dominique B, Nicolas P, Lauber M, Carnier M, Tixier-Vidal A, Cohen P (1977) Evidence for an adenylate cyclase activity in neurosecretory granule membranes from bovine neurohypophysis. Eur J Biochem 78:337–342

    Google Scholar 

  • Entman ML, Levey GS, Epstein SE (1969) Demonstration of adenyl cyclase activity in canine cardial sarcoplasmic reticulum. Biochem Biophys Res Commun 35:728–733

    Google Scholar 

  • Ernst SA (1972) Transport adenosine triphosphatase cytochemistry. II. Cytochemical localization of ouabain-sensitive, potassium dependent phosphatase activity in the secretory epithelium of the avian salt gland. J Histochem Cytochem 20:23–38

    Google Scholar 

  • Fenoglio C, Barni S, Polver P (1980) Ultrastructural cytochemical investigation on adenylate cyclase activity in isoprenaline-treated rat liver. Gegenbaurs morphol Jahrb 126:766–773

    Google Scholar 

  • Goldstein M, Anagoste B, Shirron C (1973) The effect of trivastal, haloperidol, and dibutyryl cyclic AMP on (14C) dopamine synthesis in rat striatum. J Pharm Pharmacol 25:348–351

    Google Scholar 

  • Howell SL, Whitfield M (1972) Cytochemical localization of adenyl cyclase activity in rat islands of Langerhans. J Histochem Cytochem 20:873–879

    Google Scholar 

  • Johnson RA, Welden J (1977) Characteristics of the enzymatic hydrolysis of 5′-adenylylimidodiphosphate: Implications for the study of adenylate cyclase. Arch Biochem Biophys 183:216–227

    Google Scholar 

  • McKeel DW, Jarett L (1974) The enrichment of adenylate cyclase in the plasma membrane and Golgi subcellular fractions of porcine adenohypophysis. J Cell Biol 62:231–236

    Google Scholar 

  • Kempen HJM, De Pont JJHHM, Stadhouders AM (1978) The cytochemical localization of adenylate cyclase: Fact or artefact. J Histochem Cytochem 26:298–312

    Google Scholar 

  • Lemay A, Jarett L (1975) Pitfalls in the use of lead nitrate for the histochemical demonstration of adenylate cyclase activity. J Cell Biol 65:39–50

    Google Scholar 

  • Liao S, Lin AH, Tymoczko JL (1971) Adenyl cyclase of cell nuclei isolated from rat ventral prostata. Biochim Biophys Acta 230:535–538

    Google Scholar 

  • Masur SK, Sandra K, Holtzman E, Schwartz IL, Walter R (1971) Correlation between pinocytosis and hydrosmosis induced by neurohypophyseal hormones and mediated by adenosine 3′,5′-cyclic monophosphate. J Cell Biol 49:582–594

    Google Scholar 

  • Miyamoto E, Kakiuchi S, Kakimoto Y (1974) In vitro and in vivo phosphorylation of myelin basic protein by cerebral protein kinase. Nature 249:150–151

    Google Scholar 

  • Poeggel G, Bernstein H-G (1981a) Towards a specific histochemical localization of adenylate cyclase in the rat hippocampus: Methodical aspects. Acta Histochem 69:171–175

    Google Scholar 

  • Poeggel G, Bernstein H-G, Luppa H, Bischoff D (1981b) Specific demonstration of rat brain adenylate cyclase in polyacrylamide microgels by a new histochemical procedure. Histochemistry 73:305–309

    Google Scholar 

  • De Pont JJHHM, Luyben D, Bonting SL (1979) Rat pancreas adenylate cyclase. VI. Role of the enzyme in secretine stimulated enzyme secretion. Biochim Biophys Acta 584:33–42

    Google Scholar 

  • Rechardt L, Härkönen M (1975) Electron microscopical demonstration of adenyl cyclase activities in the nervous tissue. Ultrastruct Res 50:365

    Google Scholar 

  • Reik L, Petzold GL, Higgins JA, Greengard P, Barrnett RJ (1970) Hormone sensitive adenyl cyclase: cytochemical localization in rat liver. Science 168:382–384

    Google Scholar 

  • Rostomian MA, Abramian KS, Hajos F (1981) Electron microscopic demonstration of adenylate cyclase activity in rat cortical synaptosomes. Histochemistry 70:167–172

    Google Scholar 

  • Saito T, Araki M, Akahoshi T (1980) Lead citrate as a capture reagent for the demonstration of adenylate cyclase and guanylate cyclase activities. Abstracts of the VIth International Histochemistry and Cytochemistry Congress 1980 Brighton, England, pp 106–107

  • Santolaya R, Lederis K (1980) Localization of adenylate cyclase in the neurointermediate lobe of the rat pituitary. Cell Tissue Res 207:387–394

    Google Scholar 

  • Schmidt MJ, Sokoloff L (1973) Activity of cyclic AMP-dependent microsomal protein kinase and phosphorylation of ribosomal protein in rat brain during postnatal development. J Neurochem 21:1193–1205

    Google Scholar 

  • Schulze W, Hinterberger U, wollenberger A, Krause E-G, Janiszewski E (1977) Problems of the cytochemical demonstration of adenylate cyclase. Acta Histochem Cytochem 10:371–378

    Google Scholar 

  • Soifer D, Hechter O (1971) Adenyl cyclase activity in rat liver nuclei. Biochim Biophys Acta 230:539–542

    Google Scholar 

  • Steck AJ, Appel SH (1974) Phosphorylation of myelin basic protein. J Biol Chem 249:5416

    Google Scholar 

  • Steinberg D, Huttunen JK (1972) The role of cAMP in activation of hormone-sensitive lipase of adipose tissue. In: Greengard P, Robison GA (eds) Advances in cyclic nucleotid research, vol 1. Raven Press, New York, pp 47–62

    Google Scholar 

  • Szego CM (1972) The role of cAMP in lysosome mobilization and their nucleotropic translocation in steroid hormonal target cells. In: Greengard P, Robison GA (eds) Advances in cyclic nucleotid research, Vol 1. Raven Press, New York, pp 541–564

    Google Scholar 

  • Vaes G (1968) On the mechanisms of bone resorption. The action of parathyroid hormone on the excretion and synthesis of lysosomal enzymes and on the extracellular release of acid by bone cells. J Cell Biol 39:676–697

    Google Scholar 

  • Wagner RC, Kreiner P, Barrnett RJ, Bitensky WM (1972) Biochemical and cytochemical localization of a catecholamine-sensitive adenylase cyclase in isolated capillary endothelium. Proc Natl Acad Sci USA 69:3175–3179

    Google Scholar 

  • Walzer C (1980) An attempt at localizing adenylate cyclase in rat calvaria. Influence of sodium fluoride and parathyroid hormone. Histochemistry 68:281–296

    Google Scholar 

  • Weller M, Rodnight R (1970) Stimulation by cyclic AMP of intrinsic protein kinase activity in ox brain membrane preparations. Nature 225:187–188

    Google Scholar 

  • Yamamoto T, Ozawa H (1977) Adenylate cyclase in paraneurons. A histochemical study. Arch Histol Jpn 40:49–60

    Google Scholar 

  • Yount RG, Babcock D, Ballantyne W, Ohala D (1971) Adenylyl imido-diphosphate an adenosine triphosphate analog containing a P-N-P linkage. Biochemistry 10:2484–2489

    Google Scholar 

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Authors and Affiliations

  1. Section of Biosciences, Division of Cell Sciences and Regulation, Karl Marx University, Talstraße 33, DDR-7010, Leipzig, GDR

    G. Poeggel, H. Luppa & J. Weiß

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  1. G. Poeggel
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  2. H. Luppa
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  3. J. Weiß
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Poeggel, G., Luppa, H. & Weiß, J. Multiple localizations of adenylate cyclase in rat hippocampus. Histochemistry 74, 139–147 (1982). https://doi.org/10.1007/BF00495059

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  • DOI: https://doi.org/10.1007/BF00495059

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