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Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum

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Summary

Cytochemical evidence is reported for substantiating the view that when lightly-fixed skeletal muscle is incubated in a diaminobenzidine-H2O2 medium at pH 5, the resulting enhanced electron opacity of the sarcoplasmic reticulum is more likely to be due to a peroxidatic activity therein rather than to a non-enzymic binding reaction. The reticulum staining is absent in incubated sections of overfixed or boiled tissue; or if hydrogen peroxide is omitted from the incubation medium; or if aminotriazole is included in the medium.

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Authors and Affiliations

  1. Department of Anatomy, University of Dundee, Scotland

    K. N. Christie & P. J. Stoward

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  1. K. N. Christie
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  2. P. J. Stoward
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Christie, K.N., Stoward, P.J. Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum. Histochemistry 64, 315–318 (1979). https://doi.org/10.1007/BF00495032

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  • DOI: https://doi.org/10.1007/BF00495032

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