, Volume 90, Issue 6, pp 489–496 | Cite as

Enhancement of gentamicin-induced nephrotoxicity by Mg deficiency in non-pregnant rats

Morphological, enzyme histochemical and immunohistochemical studies
  • R. Gossrau
  • T. Günther
  • R. Graf


Non-pregnant rats fed an Mg-deficient diet showed some degenerated and calcified proximal tubules in the inner region of the medullary rays accompanied by reduced or absent enzyme activities. After gentamicin treatment some damaged convoluted proximal tubules occurred. Enzyme histochemistry revealed decreased activities for brush border and lysosomal hydrolases; using immunohis-tochemistry lesions were detectable for the cytoskeletal proteins keratin and vimentin. Administration of gentamicin to Mg-deficient rats led to a further decrease of hydrolase activities in obviously intact proximal tubules and drastic structural and enzymatic defects as well as alterations of the cytoskeletal proteins in the convoluted and straight segments of other proximal tubules and to a lesser degree also in glomeruli and further portions of the tubular apparatus including the collecting ducts.


Gentamicin Proximal Tubule Brush Border Cytoskeletal Protein Hydrolase Activity 
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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • R. Gossrau
    • 1
  • T. Günther
    • 2
  • R. Graf
    • 1
  1. 1.Department of AnatomyFree University of BerlinBerlin 33Germany
  2. 2.Department of Molecular Biology and BiochemistryBerlin 33Germany

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