Skip to main content
Log in

An improved technique for the demonstration of glycogen depleted skeletal muscle fibres

  • Short Communication
  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

Glycogen depleted skeletal muscle fibres can be distinguished from non-depleted fibres with the periodic acid Schiff (PAS) reaction. In this paper the method of Meijer (1968) for the histochemical demonstration of phosphorylase activity is described as an efficient technique to increase the contrast between both groups of fibres.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Kugelberg E, Edström L (1968) Differential histochemical effects of muscle contractions on phosphorylase and glycogen in various types of fibres: relation to fatigue. J Neurol Neurosurg Psychiatry 31:415–423

    Google Scholar 

  • Lojda Z, Gossrau R, Schiebler TH (1979) Enzyme histochemistry, a laboratory manual. Springer Verlag, Berlin Heidelberg New York, pp 216–223

    Google Scholar 

  • Meijer AEFH (1968a) Improved histochemical method for the demonstration of the activity of α-glucan phosphorylase I; The use of glucosyl acceptor dextran. Histochemie 12:244–252

    Google Scholar 

  • Meijer AEFH (1968b) Improved histochemical method for the demonstration of the activity of α-glucan phosphorylase II: Relation of molecular weight of glucosyl acceptor dextran to activation of phosphorylase. Histochemie 16:134–143

    Google Scholar 

  • Pool CW, Donselaar YE, Griep PAM (1978) The α-glucan-uridine diphosphate glucosyltransferase reaction for the identification of glycogen-depleted muscle fibres. J Histochem Cytochem 26:742–744

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

To whom offprint requests should be sent

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wirtz, P., Wallinga-de Jonge, W. & Vermorken, A.J.M. An improved technique for the demonstration of glycogen depleted skeletal muscle fibres. Histochemistry 79, 141–143 (1983). https://doi.org/10.1007/BF00494351

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00494351

Keywords

Navigation