Histochemistry

, Volume 82, Issue 4, pp 351–355 | Cite as

Subcellular localization of serotonin immunoreactivity in rat enterochromaffin cells

  • O. Nilsson
  • L. E. Ericson
  • A. Dahlström
  • R. Ekholm
  • H. W. M. Steinbusch
  • H. Ahlman
Article

Summary

Serotonin immunoreactive material was localized to rat enterochromaffin cells (EC cells) at the subcellular level using antibodies to serotonin (5-HT) raised in rabbits. Ultrathin sections from paraformaldehyde fixed plastic embedded tissues were directly labelled with the 5-HT antiserum, using the protein A-gold technique to visualize the immunoreaction. The 5-HT immunoreactivity (5-HT-IR) in the rat gastrointestinal mucosa was exclusively localized to epithelial EC cells with a low background over other epithelial non-enterochromaffin cells. Quantitative evaluation of the immunoreaction revealed that most of the 5-HT-IR in the cytoplasm of EC cells (60%) was located over the dense cores of the secretory granules. However, a significant part of the cytoplasmic 5-HT-IR (40%) was located outside the dense cores of the secretory granules which suggests that different forms of 5-HT storage may exist.

Keywords

Public Health Serotonin Paraformaldehyde Subcellular Localization Quantitative Evaluation 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • O. Nilsson
    • 1
  • L. E. Ericson
    • 2
  • A. Dahlström
    • 1
  • R. Ekholm
    • 2
  • H. W. M. Steinbusch
    • 4
  • H. Ahlman
    • 3
  1. 1.Institute of NeurobiologyUniversity of GöteborgSweden
  2. 2.Department of AnatomyUniversity of GöteborgSweden
  3. 3.Department of Surgery IUniversity of GöteborgSweden
  4. 4.Department of Pharmacology, Faculty of MedicineFree UniversityAmsterdamThe Netherlands

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