Histochemistry

, Volume 91, Issue 5, pp 419–423 | Cite as

Immunocytolocalization of human gastric lipase in chief cells of the fundic mucosa

  • H. Moreau
  • A. Bernadac
  • Y. Gargouri
  • F. Benkouka
  • R. Laugier
  • R. Verger
Article

Summary

The presence in human gastric juice of a lipase secreted by the gastric mucosae has been reported previously, but its exact cellular origin has not yet been established. Polyclonal antibodies specific to human gastric lipase (HGL) were prepared, and used by an immunofluorescence technique to label cells producing HGL. This immunocytolocalization was correlated with that of pepsin (chief cells) and parietal cells using specific polyclonal or monoclonal antibodies.

Our results clearly establish that HGL is exclusively located in the chief cells of fundic mucosa; furthermore, it was found to be always co-located with pepsin. No HGL was observed in the parietal or mucus cells. HGL was always detected intracellularly, either in secretory granules of the apical region of the chief cells, or revealed by more diffuse cytoplasmic labelling.

Keywords

Public Health Monoclonal Antibody Lipase Polyclonal Antibody Gastric Juice 

Abbreviations

HGL

Human gastric lipase

SDS PAGE

Sodium dodecyl sulfate-polyAcrylamid gel electrophoresis

PBS

Phosphate buffer saline

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • H. Moreau
    • 1
  • A. Bernadac
    • 1
  • Y. Gargouri
    • 1
  • F. Benkouka
    • 3
  • R. Laugier
    • 2
  • R. Verger
    • 1
  1. 1.CNRS-Centre de Biochimie et de Biologie MoléculaireMarseille CedexFrance
  2. 2.INSERM-U315MarseilleFrance
  3. 3.INSERM-U10Hôpital BichatParisFrance

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