Summary
With in vitro autoradiography, specific receptors for atrial natriuretic peptide (ANP) were localized in fetal rat kidney and adrenal glands. Receptors were present over renal vesicles, in the primitive renal medulla and throughout the adrenal gland as early as 16 days gestation. By 20 days gestation, several layers of developing renal corpuscles were present and ANP receptors were localized over developing glomeruli in each layer. Larger accumulations occurred over the juxtamedullary glomeruli. In the medulla, the receptors were localized in a reticular pattern near the pelivis. With emulsion coated sections, ANP receptors in developing renal corpuscles were seen primarily over the lower curve of S-shaped vesicles and around the periphery of the more mature corpuscles. In the renal medulla, receptors were localized over the interstitial cells. In the 16-day-old adrenal gland, ANP receptors were present throughout the cortical area but at 20 days gestation and 1 day postpartum receptors appeared more numerous in the peripheral region. these data suggest that ANP has important developmental effects in the kidney and adrenal gland and may be involved in regulation of body fluid homeostasis in the late gestation rat fetus.
Similar content being viewed by others
References
Atarashi K, Franco-Saenz R, Mulrow PJ, Snajdar RM, Rapp JP (1984) Inhibition of aldosterone production by atrial natriuretic factor. J Hypertens 2:293–295
Barnes AC (1968) Functional differentiation of the kidney. In: Barnes AC (ed) Intra-uterine development. Lea & Febiger, Philadelphia, pp 245–252
Bianchi C, Gutkowska J, Thibault G, Garcia R, Genest J, Cantin M (1985) Radioautographic localization of 125I-atrial natriuretic factor (ANF) in rat tissues. Histochemistry 82:441–452
Bianchi C, Gutkowska J, Thibault G, Garcia R, Genest J, Cantin M (1986) Distinct localization of atrial natriuretic factor and angiotensin II binding sites in the glomerulus. Am J Physiol 251:F594-F602
Bracc RA, Cheung CY (1987) Cariovascular and fluid responses to atrial natriuretic factor in sheep fetus. Am J Physiol 253:R561-R567
Butlen D, Mistaoui M, Morel R (1987) Atrial natriuretic peptide receptors along the rat and rabbit nephrons: [125I]α-rat atrial natriuretic peptide binding in microdissected glomeruli and tubules. Pflügers Arch 408:356–365
Cantin M, Genest J (1985) The heart and the atrial natriuretic factor. Endocrinol Rev 6:107–127
Chai SY, Sexton PM, Allen AM, Figdor R, Mendelsohn FAO (1986) In vitro autoradiographic localization of ANP receptors in rat kidney and adrenal gland. Am J Physiol 250:F753-F757
Chartier L, Schiffrin EL (1986) Atrial natriuretic peptide inhibits the stimulation of aldosterone secretion by ACTH in vitro and in vivo. Proc Soc Exp Biol Med 182:132–136
Chatelain A, Dupouy JP (1981) Activity of the pituitary-adrenal system in rat fetuses subjected to encephalectomy in early or late stages of pregnancy. Neuroendocrinology 33:148–152
Chatelain A, Dupouy JP, Allaume P (1980) Fetal-maternal adrenocorticotropin and corticosterone relationships in the rat: Effects of maternal adrenalectomy. Endocrinology 106:1297–1303
Cheignon M, Bakala H, Cornet S, Djaziri R, Schaeverbeke J (1987) Localization of basement membrane glycoproteins in rat kidney during foctal development. Biol Cell 60:49–56
Cheung CY, Gibbs DM, Brace RA (1987) Atrial natriuretic factor in maternal and fetal sheep. Am J Physiol 252:E279-E282
Du Bois AM (1969) The embryonic kidney. In: Rouiller C, Muller AF (eds) The kidney: morphology, biochemistry, physiology. Academic Press, New York, pp 1–59
Ekblom P, Sariola H, Karkinen-Jääskeläinen, Saxén L (1982) The orgin of the glomerular endothelium. Cell Differ 11:35–39
Elliott ME, Goodfriend TL (1986) Atrial natriuretic peptide inhibits protein phosphorylation stimulated by angiotensin II in bovine adrenal glomerulosa cells. Biochem Biophys Res Commun 140:814–820
Ervin MG, Ross MG, Castro R, Sherman D, Lam RW, Castro L, Keske RD, Fisher DA (1988) Ovine fetal and adult atrial natriuretic factor metabolism. Am J Physiol 254:R40-R46
Forssmann WG, Hock D, Mutt V (1986) Cardiac hormones: morphology and biochemistry. Klin Wochenschr 64:4–12
Gospodarowicz D, Neufeld G, Schweigerer L (1986) Molecular and biological characterization of fibroblast growth factor, an angiogenic factor which also controls the proliferation and differentiation of mesoderm and neuroectoderm derived cells. Cell Differ 19:1–17
Healy DP, Fanestil DD (1986) Localization of atrial natriuretic peptide binding sites within the rat kidney. Am J Physiol 250:F573-F578
Herkenham M (1985) Autoradiographic methods for receptor localization. Short course 1 syllabus. Mod Neuroanat Methods Soc Neurosci pp 44–60
Idelman S (1978) The structure of the mammalian adrenal cortex. In: Jones IC, Henderson IW (eds) General, comparative and clinical endocrinology of the adrenal cortex. Academic Press, New York, pp 1–200
Jaiswal N, Paul AK, Jaiswal RK, Sharma RK (1986) Atrial natriuretic factor regulation of cyclic GMP levels and steroidogenesis in isolated fasciculata cells of rat adrenal cortex. FEBS Lett 199:121–124
Kalavsky SM (1971) Fetal rat adrenal steroidogenesis. Biol neonate 17:427–435
Kisch B (1956) Electron microscopy of the atrium of the heart I. guinea pig. Exp Med Surg 14:99–112
Klepac R, Milkovic K (1975) ACTH and growth hormone relationship in fetal rat adrenal steroidogenesis in vitro. Endokrinologie 66:74–80
Koseki C, Hayashi Y, Torikai S, Furuya M, Ohnuma N, Imai M (1986) Localization of binding sites for α-rat atrial natriuretic polypeptide in rat kidney. Am J Physiol 250:F210-F216
Lynch DR, Braas KM, Snyder SH (1986) Atrial natriuretic factor receptors in rat kidney, adrenal gland, and brain: autoradiographic localization and fluid balance dependent changes. Proc Natl Acad Sci USA 83:3357–3361
Mendelsohn FAO, Allen AM, Chai SY, Sexton PM, Figdor R (1987) Overlapping distributions of receptors for atrial natriuretic peptide and angiotensin II visualized by in vitro autoradiography: morphological basis of physiological antagonism. Can J Physiol Pharmacol 65:1517–1521
Mikhail Y (1973) Early stages in postnatal development of the adrenal gland. Acta Anat 84:138–149
Millan M, Aguilera G, Wynn PC, Mendelsohn FAO, Catt KJ (1986) Autoradiographic localization of brain receptors for peptide hormones: angiotensin II, corticotropin-releasing factor, and gonadotropin-releasing hormone. Method Enzymol 124:590–607
Murphy KMM, McLaughlin LL, Michener ML, Needleman P (1985) Autoradiographic localization of atriopeptin III receptors in rat kidney. Eur J Pharmacol 111:291–292
Needleman P (1986) Atriopeptin biochemical pharmacology. Fed Proc 45:2096–2100
Neiss WF (1982) Histogenesis of the loop of Henle in the rat kidney. Anat Embryol 164:315–330
Neiss WF, Klehn KL (1981) The postnatal development of the rat kidney with special reference of the chemodifferentiation of the proximal tubule. Histochemistry 73:251–268
Olins GM, Spear KL, Siegel NR, Zurcher-Neely HA (1987) Inactivation of atrial natriuretic factor by the renal brush border. Biochim Biophys Acta 901:97–100
Saksela O, Holthöfer H (1987) Plasminogen activators during differentiation of the human kidney. Differentiation 34:131–138
Sariola H, Peault B, LeDouarin N, Buch C, Dieterlen-Lievre F, Saxen L (1984) Extracellular matrix and capillary ingrowth in interspecies chimeric kidneys. Cell Differ 15:43–51
Saxen L (1987) Organogenesis of the kidney. In: Barlow PW, Green PB, Wylie CC (eds) Developmental and cell biology series. Cambridge University Press, New York, pp 22–25
Saxen L, Lehtonen E (1987) Embryonic kidney in organ culture. Differentiation 36:2–11
Scott JN, Jennes L (1987) Distribution of atrial natriuretic factor in fetal rat atria and ventricles. Cell Tissue Res 248:479–481
Scott JN, Jennes L (1988) Development of immunoreactive atrial natriuretic peptide in fetal hearts of spontaneously hypertensive and Wistar-Kyoto rats. Anat Embryol 178:359–363
Speller AM, Moffat DB (1977) Tubulo-vascular relationships in the developing kidney. J Anat 123:487–500
Swithers SE, Stewart RE, McCarty R (1987) Binding sites for atrial natriuretic factor (ANF) in kidneys and adrenal glands of spontaneously hypertensive (SHR) rats. Life Sci 40:1673–1681
Thompson RD, Simson JA, Currie MG (1986) Atriopeptin distribution in the developing rat heart. Anat Embryol 175:227–233
Toshimori H, Toshimori K, Oura C, Matsuo H (1987) Immunohistochemical study of atrial natriuretic polypeptides in the embryonic, fetal and neonatal rat heart. Cell Tissue Res 248:627–633
Vlasuk GP, Babilon RW, Nutt RF, Ciccarone TM, Winquist RJ (1987) The actions of atrial natriuretic factor on the vascular wall. Can J Physiol Pharmacol 65:1684–1689
Yamaji T, Hirai N, Ishibashi M, Takaku F, Yanaihara T, Nakayama T (1986) Atrial natriuretic peptide in umbilical cord blood: evidence for a circulating hormone in human fetus. J Clin Endocrinol Metab 63:1414–1417
Yamamoto I, Ogura T, Ota Z (1987) In vitro macro-and microautoradiographic localization of atrial natriuretic peptide in the rat kidney. Res Commun Chem Pathol Pharmacol 56:185–198
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Scott, J.N., Jennes, L.H. Ontogeny of atrial natriuretic peptide receptors in fetal rat kidney and adrenal gland. Histochemistry 91, 395–400 (1989). https://doi.org/10.1007/BF00493826
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00493826