Abstract
This study investigated the possibility that Candida albicans components exert a suppressive effect on the immune response of guinea pigs (GP), similar to that of live C. albicans organisms as was previously shown. Hartley GP were inoculated with C. albicans crude cytoplasmic or metabolite (culture filtrate) extracts (containing most of the organism's cell contents or its metabolite and degradation products, respectively). Their immunological responses towards sheep red blood cells (SRBC) were compared with those of GP inoculated with SRBC alone or with SRBC together with live C. albicans organisms. The immunological responses were measured by: 1) rosette formation (RF) of SRBC with peritoneal macrophages, 2) haemolytic plaque formation (PFC) with lymph node-cells, 3) haemagglutination and 4) haemolysis tests. According to the RF tests, inoculation of GP with either cytoplasmic or metabolite extracts resulted in descreased RF as compared to GP inoculated with SRBC only; the decrease was correlated with the protein concentration of the extracts. Inoculation with metabolite extract led to a more diminished RF than with cytoplasmic extract, but less than with live C. albicans organisms. Inoculation of cytoplasmic extracts did not affect the haemagglutinin and haemolysin titers, while that of metabolite extracts resulted in a slight decrease of these titers. The assays for PFC were not conclusive enough to point to a suppressive effect of C. albicans extracts. In summary, it appears that both the cytoplasmic and metabolite extracts of C. albicans exert a partial suppressive effect on the immune response in GP, as judged primarily on the basis of the RF results.
Similar content being viewed by others
References
Barker, L.R. 1971. Experimental malaria. Effect upon the immune response to different antigens. J. Infect. Dis. 123: 99–101.
Bennett, R.H. & D.E. Jasper. 1977. Immunosuppression of humoral and cell mediated responses in calves associated with inoculation of Mycoplasma bovis. Am. J. Vet. Res. 38: 1731–1738.
Boyden, S.V. 1964. Cytophilic antibodies in guinea pigs with delayed type hypersensitivity. Immunology 7: 474–483.
Crandall, C.A. & R.B. Crandall. 1976. Ascaris suum: Immunosuppression in mice during acute infection. Exp. Parasitol. 40: 363–372.
Dent, P.B. 1972. Immunosuppression by oncogenic viruses. Prog. Med. Virol. 14: 1–35.
Dessaint, J.P., D. Camus & L.A. Capron. 1977. Depression de la proliferation lymphocytaire par un facteur libe par Schistosoma mansoni. Ann. Immunol. 128c: 57–58.
Faubert, G. & C.E. Tanner. 1971. Trichinella spiralis: inhibition of sheep haemagglutinins in mice. Exp. Parasitol. 30: 120–123.
Finger, H., B. Heymer, H. Hof, H. Meister, B. Buttmann & P.O. Haferkam. 1974. Studies on an immunosuppressant present in cytoplasmic preparations of B hemolytic Streptococci. Z. immun. Forsch. 147: 449–464.
Fischer, A., J. J. Ballet & C. Grisceli. 1978. Specific inhibition of in vitro Candida induced lymphocyte proliferation by polysaccharidic antigens present in serum of patients with chronic mucocutaneous candidiasis. J. Clin. Invest. 621: 1005–1012.
Good, A.H. & K.L. Miller. 1976. Depression of the immune response to sheep erythrocytes in mice infected with Taenia crassiceps larvae. Infect. Immun. 14: 449–456.
Jakoniuk, P., W. Jablonska & J. Borowski. 1972. The effect of Candida albicans infection on the immunological response in mice. Gruzlica, 40: 539–541.
Jerne, N.K. & A.A. Nordin. 1963. Plaque formation in agar by single antibody producing cells. Science 140: 405.
Jones, J.F., C.A. Crandall & R.B. Crandall. 1976. Tdependent suppression of the primary antibody response to sheep erythrocytes in mice infected with Trichinella spiralis. Cell. Immunol. 27: 102–110.
Lowry, O.H., N.J. Rosenbrough, A.L. Farr& R.J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265–275.
Malakian, A.H. & J.M. Schwab. 1971. Biological characterization of an immunosuppressant from group A Streptococci. J. Exp. Med. 134: 1253–1265.
Marcus, L. & H.J. Halvorson. 1967. Resolution and isolation of yeast polysomes. In Methods of Enzymology. XII A, p. 498–502. Academic Press, New York and London.
Miller, G.H. & R.W. Jackson. 1973. The effect of Streptococcus pyogenes teichoic acid on the immune response of mice. J. Immunol. 110: 148–156.
Paterson, P.Y., R. Semo, G. Blumenchein & J. Swelstael. 1971. Mucocutaneous candidiasis. Anergy and plasma inhibition of cellular immunity. Reversal after amphotericin B. therapy. Clin. Exp. Immunol. 9: 595–602.
Rogers, T.J. & L.E. Balish. 1978. Suppression of lymphocyte blastogenesis by Candida albicans. Clin. Immunol. Pathol. 10: 298–305.
Rogers, T.J. & E. Balish. 1978. Effect of systemic candidiasis on blastogenesis of lymphocytes from germfree and conventional rats. Infect. Immun. 20: 142–150.
Schwab, J.H. 1975. Suppression of the immune response by microorganisms. Bacteriol. Rev. 39: 121–143.
Segal, E., N. Vardinon, J. Schwartz & E. Eylan. 1975. Mast cell sensitizing antibodies in experimental candidiasis. Acta Allerg. 30: 1–8.
Segal, E., J. Schwartz, Z. Altboum, N. Vardinon & E. Eylan. 1977. Suppressive action of Candida albicans on me immune response in guinea pigs. Microbios 19: 79–87.
Vardinon, N. & E. Segal. 1979. Suppressive action of Candida albicans on the immune response in mice. Exp. Cell. Biol. 47: 275–280.
Wilkens, H.A. & J. Brown. 1977. Schistosoma haematobium in a Gambian community. II. Impaired cell mediated immunity and other immunological abnormalities. Ann. Trop. Med. Parasitol. 71: 59–65.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Segal, E., Sandovsky-Losica, H. & Vardinon, N. Suppressive action of cytoplasmic and metabolite extracts of Candida albicans on the immune response in guinea pigs. Mycopathologia 72, 121–128 (1980). https://doi.org/10.1007/BF00493821
Issue Date:
DOI: https://doi.org/10.1007/BF00493821