Abstract
A study of the genus Saccharomyces by new biocharacters demonstrates indirectly the genotype of each species; intracellular osidases (lactose — maltose — cellobiase — melibiase — invertase — trehalase) and vitamine requirements (biotin — folic acid — inositol — niacine — pantothénate —para-aminobenzoïc acid — pyridoxine — thiamine) reveal some phylogenetic links between the species. It appears that Van der Walt's group I is very homogeneous and must be restricted to only 7 species. The Torulospora group seems very similar to the genus Debaryomyces when vitamine needs and osidases are considered; the relations between the 2 groups are discussed. When other biocharacters are studied (nitrite reductase-immunological properties, etc. ...) the value in the GC content (4–5% lower in Debaryomyces) will be established.
These results confirm that intracellular osidases and vitamine needs are very valuable tools for systematic criteria.
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Références
Barker, B. T. P. 1901. A conjugating yeasts (Zygosaccharomyces n. gen.) Phil. Transact. Royal. Soc. (ser. B) 194: 467–483.
Fiol, J. B. 1967. Interêt systématique des tests de croissance en milieu déficient en vitamines pour l'étude des genres Kluyveromyces et Pichia. Rev. Mycol., 32: 45–56.
Fiol, J. B. 1972. Recherche d'enzymes intracellulaires chez les Kluyveromyces. Conséquences taxonomiques. Mycopath. Mycol. Applicata, 48: 303–321.
Fiol, J. B. 1973. Activité enzymatique des Ascomycètes: Pichia vini et P. vini var. melibiosi. Etude particulière de l'α galactosidase et de la β glucosidase. Conséquences taxonomiques. Mycopath. mycol. Applicata, 51: 207–213.
Fiol, J. B. 1973. Biosystématique des levures: recherche d'osidases besoins vitaminiques. teneur en guanine cytosine des ADN. Thèse d'état de l'université de Lyon I, 217 pages.
Fiol, J. B. 1974. Biosystématique du genre Pichia: recherche d'enzymes intracellulaires. Conséquences taxonomiques. Mycopath. Mycol. Applicata. 54: 267–283.
Fiol, J. B. 1975. A critical study of the taxonomic value of some tests of assimilation used for the classification of the sporogenous yeasts. Mycopath. Mycol. Applicata 57(2): 79–88.
Kreger van Rij, N. J. W. 1970. Pichia hansen in the yeast J. Lodder ed. 454–554.
Kurtzman, C. P., M. J. S. Smiley & F. Baker. 1975. Scanning electron microscopy of ascospores of Debaryomyces and Saccharomyces. Mycopathologia 55: 129–34.
Lodder, J. & N. J. W. Kreger van Rij. 1952. The yeast: a taxonomical study. North Holland Publishingc C. Amsterdam.
Nakase, T. & K. Komagara. 1971. Significance of DNA base composition of genus Debaryomyces. J. Gen. Appl. Microbiol. 17: 43–50.
Phaff, H. J. 1956. A proposal amendment of the diagnosis of the genus Pichia Hansen. Antonie. Van. Leeuw. 22: 113–116.
Poncet, S. & J. B. Fiol. 1972. Taxonomy of Kluyveromyces. Evaluation of the DNA base composition. 38: 145–152.
Van der Walt, J. P. 1970. Saccharomyces Meyer emend Rees. in the Yeast. J. Lodder ed. 555–718.
Van der Walt, J. P. & E. Johannsen. 1975. The genus Torulaspora (Lindner) Microbiology Research group. Bulletin 2: 1–23.
Wickerham, L. J. 1969. Yeast taxonomy in relation to ecology, genetics and phylogeny. Antonie Van Leeuw. 35, supplement yeast symposium 31–58.
Yarrow, P. and T. Nakase. 1975. Dna base composition of species of the genus Saccharomyces. Antonie Van Leeuw., 1: 81–88.
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Collaboration technique: Mme Billon-Grand.
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Fiol, J.B. Systématique des Saccharomyces: Osidases et besoins vitaminiques. Mycopathologia 58, 49–58 (1976). https://doi.org/10.1007/BF00493593
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DOI: https://doi.org/10.1007/BF00493593