Summary
The histochemical activities of succinic dehydrogenase (SDH), myofibrillar Adenosine triphosphatase (ATPase) and alpha glycerophosphate dehydrogenase were studied in serial sections of rat vastus lateralis (red) (RVL), gastrocnemius and diaphragm muscles. Three main fibre-types were distinguished. The “Type I” fibres of RVL and gastrocnemius muscles fell into two distinct groups: one category-”Type IA” showed very low ATPase activity. The second category of “Type IB” fibres displayed moderate ATPase reaction. The “Type IA” fibres were divisible into two sub-groups when tested for SDH reaction. “Type IA1” fibres possessed a homogenous distribution of diformazan·granules throughout the fibre: “Type IA2” fibres displayed characteristic “moth-eaten” pattern of diformazan localization. The diaphragm muscle did not show either “Type IB” or “Type IA2” varieties. The great majority of Type I fibres were sub-type IA1 in the three fast muscles studied. It is also demonstrated here that an inherent heterogeneity exists between Type I fibres of diaphragm and leg muscles in regard to α-GPD localization. This histochemical data emphasizes the fact that subdivision of Type I striated muscle fibres of mammalian animals into two sub-types is only approximate and that a further subcategorization is possible.
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Askanas, V., Engel, W.K.: Distinct sub-types of Type I fibres of human skeletal muscle. Neurology (Minnea..) 25, 879–887 (1975)
Barka, T., Anderson, P.: Histochemistry, theory, practice and bibliography, p. 313. New York: Harper and Row Publ. Inc. 1963
Brooke, M.H., Kaiser, K.K.: Muscle fibre types: How many and what kind: Arch. Neurol. (Chic.) 23, 369–379 (1970)
Brooke, M.H., Williamson, E., Kaiser, K.K.: The behaviour of four fiber-types in developing and reinnervated muscle. Arch. Neurol. (Chic.) 25, 360–366 (1971)
Dubowitz, V., Brooke, M.H.: Muscle biopsy: a modern approach. London: W.B. Saunders, Co. Ltd. 1973
Guth, L., Samaha, F.J.: Qualitative differences between actomyosin ATPase of slow and fast mammalian muscle. Exp. Neurol. 25, 138–152 (1969)
Karpati, G., Carpenter, S., Eisen, A.A.: Experimental core-like lesions and nemaline rods. Arch. Neurol. (Chic.) 27, 237–251 (1972)
Karpati, G., Eisen, A.A., Carpenter, S.: Sub-types of the histochemical type I muscle fibres. J. Histochem. Cytochem. 23, 89–91 (1975)
Khan, M.A., Papadimitriou, J.M., Holt, P.G., Kakulas, B.A.: A calcium-citro-phosphate technique for the histochemical localization of myosin ATPase. Stain Technol. 47, 277–281 (1972)
Khan, M.A., Papadimitriou, J.M., Holt, P.G., Kakulas, B.A.: Further histochemical properties of rabbit skeletal muscle fibres. Histochemie 36, 173–183 (1973)
Khan, M.A., Papadimitriou, J.M., Kakulas, B.A.: The effect of temperature on the pH stability of myosin ATPase as demonstrated histochemically. Histochemistry 38, 181–194 (1974)
Khan, M.A.: The histochemical characterization of fibre-types in mammalian fast and slow striated muscles, p. 326, 10th Int. Cong. Anat. (Abstracts). (Ed. E. Yamada) Tokyo: 1975
Khan, M.A.: Histochemical characteristics of vertebrate striated muscle: A review. Prog. Histochem. Cytochem. 8, 1–48 (1976a)
Khan, M.A.: Histochemical sub-types of three fibre-types of avian skeletal muscle. Histochemistry 50, 9–16 (1976b)
Khan, M.A.: On the subsarcolemmal localization of phenazine ethosulfate-linked α-glycerophosphate dehydrogenase activity in pigeon pectoralis white muscle fibres. Histochemistry 50, 103–110 (1976c)
Khan, M.A., Papadimitriou, J.M., Kakulas, B.A.: The effect of EDTA on the histochemical myofibrillar ATPase reaction. Acta Histochem. Suppl. 16, 281–290 (1977)
Khan, M.A., Kleine, T.O.: Histochemical and biochemical investigations of adenosine triphosphatase in vertebrate mixed muscles. Acta Histochem. Suppl. 18, 245–258 (1977)
Khan, M.A.: The histoenzymology of striated muscle fibers: an overview. Int. J. Cell & Molec. Biol. In Press (1977)
Koenig, J., Fardeau, M.: Etude histochimique des muscles grands dorsaux anterieur et posterieur du poulet et des modifications observees apres denervation et reinnervation homologue ou croisee. Arch. Anat. Microsc. 62, 249–267 (1973)
Kovarsky, J., Schochet, S.S., McCormick, W.F.: The significance of target fibres. A clinicopathologic review of 100 patients with neurogenic atrophy. Amer. J. Clin. Pathol. 59, 790–797 (1973)
Kubista, V., Kubistova, J., Pette, D.: Thyroid hormone induced changes in the enzyme activity pattern of energy-supplying metabolism of fast (white), slow (red), and heart muscle of the rat. Europ. J. Biochem. 18, 553–560 (1971)
Meijer, A.E.F.H.: Histochemical method for the demonstration of myosin adenosine triphosphatase in muscle tissue. Histochemie 22, 51–58 (1970)
Meltzer, H.Y.: Central core fibres in an acutely psychotic patient. Arch. Gen. Psychiat. 27, 125–132 (1972)
Meyers, K.R., Gilden, D.H., Rinaldi, C.F., Hansen, J.L.: Periodic muscle weakness, normokalemia and tubular aggregates. Neurology (Minneap.) 22, 269–279 (1972)
Padykula, H.A., Herman, E.: Factors affecting the activity of adenosine triphosphatase and other phosphatases as measured by histochemical techniques. J. Histochem. Cytochem. 3, 170–175 (1955)
Ringqvist, M.: Histochemical enzyme profiles of fibres in human masseter muscles with special regard to fibres with intermediate myofibrillar ATPase reaction. J. Neurol. Sci. 18, 133–141 (1973)
Shafiq, S.A., Askanas, V., Milhorat, A.T.: Fiber types and preclinical changes in chicken muscular dystrophy. Arch. Neurol. (Chic.) 25, 560–571 (1971)
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Khan, M.A. Histoenzymatic characterization of sub-types of Type I fibres in fast muscles of rats. Histochemistry 55, 129–138 (1978). https://doi.org/10.1007/BF00493515
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DOI: https://doi.org/10.1007/BF00493515