Summary
The intermediate filament protein keratin 16 is expressed in hyperproliferative epidermis. The present study aims to clarify the relationship between the expression of this keratin type, hyperproliferation (percentage of cells in SG2M phases), and keratinization (keratin 10 expression). These three parameters were quantified in biopsy material taken at different time intervals following sellotape stripping — this being a dynamic in vivo model for the induction of hyperproliferation. From the biopsy specimens cell suspensions were prepared, labeled with antibodies Ks8.12 (specially directed against keratin 16) and RKSE60 (directed against keratin 10), and analyzed using flow cytometry. Percentages of cells in SG2M phases were assessed by measuring the relative DNA content after propidium iodide staining. Keratin 16 expression in the suprabasal layer anticipated epidermal proliferation, suggesting a role of the suprabasal compartment in the induction of epidermal growth. Keratin 10 expression decreased about 1 day after the onset of keratin 16 expression, indicating that these processes do not depend directly upon each other.
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References
Akhurst RJ, Fee F, Balmain A (1988) Localized production of TGF-Β mRNA in tumor promoter-stimulated mouse epidermis. Nature 331:363–365
Baisch H, Linden WA (1975) Different mathematical models for pulse-cytophotometric evaluations applied to asynchronous and partially synchronized cell populations. In: Haanen CAM, Hillen HFP, Wessels JMC (eds) Pulse cytophotometry, part I. European Press Medikon, Gendt, pp 61–75
Bauer FW, Boezeman JBM (1983) Flow cytometric methods in human skin with respect to cell cycle kinetics. In: Wright NA, Camplejohn NS (eds) Psoriasis: cell proliferation. Churchill Livingstone, Edinburgh, pp 104–116
Bauer FW, Crombag NHCMN, De Grood RM, De Jongh GJ (1980) Flow cytometry as a tool for the study of cell kinetics in epidermis. Br J Dermatol 102:629–639
Boezeman JBM, Bauer FW, de Grood RM (1987) Flow cytometric analysis of the recruitment of Go cells in human epidermis in vivo following tape stripping. Cell Tissue Kinet 20:99–104
De Mare S, Van Erp PEJ, Van de Kerkhof PCM (1989) Epidermal hyperproliferation assessed by the monoclonal antibody Ks8.12 on frozen sections. J Invest Dermatol 92:130–131
Eichner R, Weiss RA, Torres A, Sun TT (1987) Keratin expression in psoriatic and tape-stripped human epidermis. In: Farber EM, Nall L, Morhenn V, Jacobs PH (eds) Psoriasis: proceedings of the fourth international symposium. Elsevier, New York, pp 36–41
Huszar M, Gigi-Leitner O, Moll R, Franke WW, Geiger B (1986) Monoclonal antibodies to various acidic (type I) cytokeratins of stratified epithelia. Differentiation 31:141–153
Iversen OH (1985) What's new in proliferation and differentiation in malignant tumors? Pathol Res Pract 180:705–710
Lane EB, Bártek J, Purkis PE, Leigh EM (1985) Keratin antigens in differentiating skin. Ann NY Acad Sci 455:241–258
Mansbridge JN, Knapp AM (1987) Changes in keratinocyte maturation during wound healing. J Invest Dermatol 89:253–263
Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalogue of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24
Moll R, Moll I, Franke WW (1984) Differences of expression of cytokeratin polypeptides in various epithelial skin tumors. Arch Dermatol Res 276:349–363
Morhenn VB (1988) Keratinocyte proliferation in wound healing and skin diseases. Immunol Today 9:104–107
Pinkus H (1951) Examination of the epidermis by the strip method. II. Biometric data on regeneration of the human epidermis. J Invest Dermatol 19:431–447
Ramaekers FCS, Puts JJG, Moesker O, Kant A, Huysmans A, Haag D, Jap PHK, Herman CJ, Vooijs GP (1983) Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview. Histochem J 15:691–713
Schermer A, Galvin S, Sun TT (1986) Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 103:49–62
Stoler A, Kopan R, Duvic M, Fuchs E (1988) Use of monospecific antisera and cRNA probes to localize the major changes in keratin expression during normal and abnormal epidermal differentiation. J Cell Biol 107:427–446
Van Erp PEJ, Rijzewijk JJ, Boezeman JBM, Leenders J, de Mare S, van de Kerkhof PCM, Ramaekers FCS, Bauer FW (1989) Flow cytometric analysis of epidermal subpopulations from normal and psoriatic skin using monoclonal antibodies against intermediate filaments. Am J Pathol (in press)
Van Muijen GNP, Warnaar SO, Ponec M (1987) Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis. Exp Cell Res 171:331–345
Weiss RA, Eichner R, Sun TT (1984) Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48-and 56-kDalton keratin as molecular markers for hyperproliferative keratinocytes. J Cell Biol 98:1397–1406
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de Mare, S., van Erp, P.E.J., Ramaekers, F.C.S. et al. Flow cytometric quantification of human epidermal cells expressing keratin 16 in vivo after standardized trauma. Arch Dermatol Res 282, 126–130 (1990). https://doi.org/10.1007/BF00493471
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DOI: https://doi.org/10.1007/BF00493471