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The determination by microdensitometry of the initial maximum velocity rate of rat ovarian 3β hydroxysteroid dehydrogenase activity

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Summary

The determination of the initial maximum velocity rate of 3β hydroxysteroid dehydrogenase activity (3β HSD) of the newest generation of corpora lutea of the adult dioestrous rat ovary is described. Under conditions where the substrate and co-factor concentrations are not rate limiting (4×10−4 M epiandrosterone; 3×10−3 M NAD+ respectively) the initial maximum reaction rate was maintained for approximately 6 min. NADH production during this period was 4.9±0.2 μmoles/min/cm3 of corpora lutea.

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Authors and Affiliations

  1. Department of Chemical Pathology, University of Manchester, Clinical Sciences Building, Hope Hospital, Eccles Old Road, M6 8HD, Salford, UK

    W. R. Robertson, R. J. Earnshaw & A. Lambert

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  1. W. R. Robertson
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  2. R. J. Earnshaw
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  3. A. Lambert
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Robertson, W.R., Earnshaw, R.J. & Lambert, A. The determination by microdensitometry of the initial maximum velocity rate of rat ovarian 3β hydroxysteroid dehydrogenase activity. Histochemistry 74, 435–442 (1982). https://doi.org/10.1007/BF00493442

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  • DOI: https://doi.org/10.1007/BF00493442

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