, Volume 73, Issue 4, pp 625–634 | Cite as

Examination of chronically de-efferented cat muscle spindles for cholinesterase activity

  • J. Kucera


Cat tenuissmus muscles were deprived of motor nerve supply for three months by sectioning of the appropriate ventral spinal roots. Muscle spindles were located in the chronically de-efferented muscles and examined histochemically in serial transverse sections. Staining for nicotinamide adenine dinucleotide tetrazolium reductase showed that the spindle sensory innervation was preserved. The de-efferented intrafusal muscle fibers retained their differential staining with the reaction for myosin adenosine triphosphatase. However, all cholinesterase-active areas that are normally found along nuclear bag and nuclear chain intrafusal fibers demonstrated loss of the enzyme activity in the chronically de-efferented spindles. It is concluded that all histochemically demonstrable cholinesterase activity within the cat muscle spindle is dependent upon the continuous presence of motor innervation.


Nicotinamide Adenine Dinucleotide Nicotinamide Adenine Muscle Spindle Cholinesterase Activity Adenosine Triphosphatase 
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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • J. Kucera
    • 1
  1. 1.Department of Neurology, School of MedicineBoston UniversityBostonUSA

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