, Volume 62, Issue 2, pp 153–157 | Cite as

Cryo-ultramicrotomy after coating of the cut surface of the tissue block with carbon and metals before sectioning

  • A. Schiller
  • R. Taugner


Working under improvised conditions it is shown that coating of the cut surface of a frozen tissue block with metal and carbon improves, when applied prior to each cutting, the sectioning properties and the handling of the subsequent ultrathin cryosection.


Public Health Freeze Tissue Tissue Block Section Property Ultrathin Cryosection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Ali, S.Y., Gray, J.C., Wisby, A., Phillips, M.: Preparation of thin cryo-sections for electron probe analysis of calcifying cartilage. J. Microsc. 111, 65–76 (1977)Google Scholar
  2. Appleton, T.C.: A cryostat approach to ultrathin “dry” frozen sections for electron microscopy: A morphological and X-ray analytical study. J. Microsc. 100, 49–74 (1974)Google Scholar
  3. Baker, J.R.J., Appleton, T.C.: A technique for electron microscopy autoradiography (and X-ray microanalysis) of diffusible substances using freeze-dried fresh frozen sections. J. Microsc. 108, 307–315 (1976)Google Scholar
  4. Barnard, T., Sevéus, L.: Preparation of biological material for X-ray microanalysis of diffusible elements. II. Comparison of different methods of drying ultrathin cryosections cut without a through liquid. J. Microsc. 112, 281–291 (1978)Google Scholar
  5. Bernhard, W., Viron, A.: Improved techniques for the preparation of ultrathin frozen sections. J. Cell Biol. 49, 731–746 (1971)Google Scholar
  6. Boll, H.U., Eschwey, J., Reb, G., Taugner, R.: A section stretching apparatus for ultracryotomy. Experientia 31, 618–619 (1975)Google Scholar
  7. Boll, H.U., Reb, G., Taugner, R.: Synthetic coating: An improvement in ultracryotomy. Experientia 30, 1103–1104 (1974)Google Scholar
  8. Christensen, A.K.: Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver. J. Cell Biol. 51, 772–804 (1971)Google Scholar
  9. Fuchs, W., Lindemann, B., Brombach, J.D., Trösch, W.: Instrumentation and specimen preparation for electron beam X-ray microanalysis of frozen hydrated bulk specimens. J. Microsc. 112, 75–87 (1978)Google Scholar
  10. Geymayer, W., Grasenick, F., Hödl, Y.: Stabilizing ultrathin cryo-sections by freeze-drying. J. Microsc. 112, Pt 1 39–46 (1978)Google Scholar
  11. Hellström, S., Sjöström, M.: An anti-roll plate for flattening of ‘dry’ ultrathin frozen sections. J. Microsc. 101, 197–200 (1974)Google Scholar
  12. Hodson, St., Marshall, J.: A device for trimming frozen biological tissue preparatory to ultra-thin sectioning. J. Microsc. 89, Pt 3, 373–375 (1969)Google Scholar
  13. Hodson, St., Marshall, J.: Ultracryotomy: a technique for cutting ultrathin sections of unfixed frozen biological tissues for electron microscopy. J. Microsc. 91, 105–117 (1970)Google Scholar
  14. Iglesias, J.R., Bernier, R., Simard, R.: Ultracryotomy: A routine procedure. J. Ultrastruct. Res. 36, 271–289 (1971)Google Scholar
  15. Marshall, A.T.: Electron probe X-ray microanalysis of frozen-hydrated biological specimens. Microsc. Acta 79, 254–266 (1977)Google Scholar
  16. Saetersdal, T.S., Røli, J., Myklebust, R., Engedal, H.: Preservation of shock-frozen myocardial tissue as shown by cryo-ultramicrotomy and freeze-fracture studies. J. Microsc. 111, 297–311 (1977)Google Scholar
  17. Saubermann, A.J., Echlin, P.: The preparation, examination and analysis of frozen hydrated tissue sections by scanning transmission electron microscopy and X-ray microanalysis. J. Microsc. 105, 155–191 (1975)Google Scholar
  18. Schiller, A., Sonnhof, U., Taugner, R.: Die Anwendung von Kryoprotektiva für Kryo-Ultramikrotomie und Gefrierbruchtechnik unter Kontrolle der Gewebefunktion. 10. Kolloquium des Arbeitskreises für Elektronenmikroskopische Direktabbildung und Analyse von Oberflächen (EDO) vom 4.-9.9.1977 in Münster/Westfalen. Beitr. elektronenmikroskop. Direktabb. Oberfl. 10, (1978)Google Scholar
  19. Schiller, A., Taugner, R., und Rix, E.: Die Anwendung kryoprotektiver Substanzen in der Gefrierbruchtechnik. 1. Gefrierätzseminar bei Leybold-Heraeus, Köln, 10. bis 12. Mai 1977. Mikroskopie (Wien) 34, 19–23 (1978)Google Scholar
  20. Sevéus, L.: Preparation of biological material for X-ray microanalysis of diffusible elements. I. Rapid freezing of biological tissue in nitrogen slush and preparation of ultrathin frozen sections in the absence of through liquid. J. Microsc. 112, 269–279 (1978)Google Scholar
  21. Tokuyasu, K.T.: A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol. 57, 551 (1973)Google Scholar
  22. Trump, B.F., Berezesky, I.K., Chang, S.H., and Bulger, R.E.: Detection of ion shifts in proximal tubule cells of the rat kidney using X-ray microanalysis. Virchows Arch. B Cell Pathol. 22, 111–120 (1976)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • A. Schiller
    • 1
  • R. Taugner
    • 1
  1. 1.Institute of Physiology IUniversity of HeidelbergHeidelbergFederal Republic of Germany

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