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Histochemistry

, Volume 72, Issue 4, pp 579–615 | Cite as

NADP-dependent dehydrogenases in rat liver parenchyma

III. The description of a liponeogenic area on the basis of histochemically demonstrated enzyme activities and the neutral fat content during fasting and refeeding
  • H. Rieder
Article

Summary

The activities of glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), malic enzyme (ME) and isocitrate dehydrogenase (ICDH) were investigated with optimized histochemical methods (Rieder et al. 1978), and the activity of 3-hydroxybutyrate dehydrogenase (3HBDH) and neutral fat content with conventional techniques in the liver of male rats under the following experimental dietary conditions: (A) Fasting for 0, 12 and 84 h; (B) 84-h fasting followed by refeeding with a low-fat, high-carbohydrate diet for 6 h and for 2, 3, 5, 7, 11 and 14 nights; (C) refeeding with standard diet for 5 nights; (D) low-fat, high-carbohydrate diet for 7 and 14 nights.

The activities of G6PDH, 6PGDH and ME decreased slightly during fasting primarily in zone 1 and increased dramatically on refeeding with a low-fat, high-carbohydrate diet. This activity increase was confined mainly to zone 3 during the first 3 days and was accompanied by a deposition of neutral fats that began in zone 3 and progressed to zone 1. Neutral fat accumulation was maximal after 3 nights, with a uniform accumulation of large droplets in all the hepatocytes; this was followed by a release that started in zone 3 and proceeded in a periportal direction. On the other hand, G6PDH, 6PGDH and ME attained their maximum activities after 5 and 7 nights of the low-fat diet, the activities being nearly homogeneously distributed over the liver acinus in a few cases. Subsequently the activities fell mainly in zone 1, causing the activity patterns and levels to approach those of the animals in group (D). In contrast to this, the activity of ICDH increased during fasting principally in zone 1, so that the otherwise steep activity gradient in favor of zone 3 lessened. Refeeding led at first to a fall of activity below the initial value, but later the normal distribution pattern was restored. The activity of 3HBDH showed a behavior similar to that of ICDH. The findings are discussed with reference to the functional heterogeneity of the liver perenchyma, and the existence of a liponeogenic area in zone 3 is proposed.

Keywords

Liver Parenchyma Isocitrate Malic Enzyme Large Droplet Functional Heterogeneity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Afolabi SK, Kissebah A, Vydelingum N, Tulloch BR, Fraser RT (1974) The mechanism of hypertriglyceridaemia in oestrogen-treated rats. J Endocrinol 63:58pGoogle Scholar
  2. Allmann W, Hubbard DD, Gibson DM (1965) Fatty acid synthesis during fat-free refeeding of starved rats. J Lipid Res 6:63–74Google Scholar
  3. Altman FP, Høyer PE, Andersen H (1979) Dehydrogenase histochemistry of lipid-rich tissues: a tetrazolium-metal chelation technique to improve localization. Histochem J 11:485–488Google Scholar
  4. Aterman K, Altmann H-W (1979) Das intrahepatische Gallenwegssystem. In: Kühn HA, Wernze H (eds) Klinische Hepatologie. Georg Thieme Stuttgart, pp 1.53–1.62Google Scholar
  5. Badwey JA, Karnovsky ML (1980) Active oxygen species and the functions of phagocytic leukocytes. Annu Rev Biochem 49:695–726Google Scholar
  6. Berkel TJC van, Kruijt JK (1977) Distribution and some properties of NADPH and NADH oxidase in perenchymal and non-parenchymal liver cells. Arch Biochem Biophys 179:8–14Google Scholar
  7. Bloch K, Vance D (1977) Control mechanisms in the synthesis of saturated fatty acids. Annu Rev Biochem 46:263–298Google Scholar
  8. Brunengraber H, Boutry M, Lowenstein JM (1973) Fatty acid and 3-β-hydroxysterol synthesis in the perfused rat liver. J Biol Chem 248:2656–2669Google Scholar
  9. Buckley BM, Williamson DH (1973) Acetoacetate and brain lipogenesis: developmental pattern of acetoacetyl-coenzyme A synthetase in the soluble fraction of rat brain. Biochem J 132:653–656Google Scholar
  10. Buckley BM, Williamson DH (1975) Acetoacetyl-CoA synthetase; a lipogenic enzyme in rat tissue. FEBS Lett 60:7–10Google Scholar
  11. Burch H, Lowry OH, Kuhlman AM, Skerjance J, Diamant EJ, Lowry StR, von Dippe P (1963) Changes in patterns of enzymes of carbohydrate metabolism in the developing rat liver. J Biol Chem 238:2267–2273Google Scholar
  12. Burstone MS (1959) New histochemical techniques for the demonstration of tissue oxidase (cytochrome oxidase). J Histochem Cytochem 7:112–122Google Scholar
  13. Burton DN, Collins JM, Kennan AL, Porter JW (1969) The effects of nutritional and hormonal factors on the fatty acid synthetase level of rat liver. J Biol Chem 244:4510–4516Google Scholar
  14. Butcher RG (1971) The effect of phenobarbitone on the production of type I hydrogen from reduced nicotinamide-adenine dinucleotide phosphate in different regions of the liver. Biochem J 125:22p-23pGoogle Scholar
  15. Cain H (1968) Über die Fettleber aus morphologischer Sicht. In: Demling L, Berg G (eds) Fettleber. Pallas, Lochham bei München, S 5–11Google Scholar
  16. Carlson SE, Mitchell AD, Goldfarb S (1978) Sex-related differences in diurnal activities and development of hepatic microsomal 3-hydroxy-3-methyl-glutaryl coenzyme A reductase and cholesterol 7 α-hydroxylase. Biochim Biophys Acta 531:115–124Google Scholar
  17. Carlson SE, Mitchell AD, Goldfarb S (1979) Regulation of hepatic HMG-CoA reductase activity. Fed Proc 38:5416Google Scholar
  18. Chang MLW, Johnson MA (1976) Stoppage of glycogenesis and “over-shoot” of induction of lipogenesis and its related enzyme activities in the liver of fasted-refed rat. J Nutr 106:136–141Google Scholar
  19. Clark DG, Rognstad R, Katz J (1974) Lipogenesis in rat hepatocytes. J Biol Chem 249:2028–2036Google Scholar
  20. Craig MC, Neprokoeff CM, Lakshmanan MR, Porter JW (1972) Effect of dietary change on the rates of synthesis and degradation of rat liver fatty acid synthetase. Arch Biochem Biophys 152:619–630Google Scholar
  21. Curthoys NP, Hughey RP, Coyle PJ (1978) The membrane association and physiological function of rat renal γ-glutamyltranspeptidase. In: Sies H, Wendel A (eds) Functions of glutathione in liver and kidney. Springer, Berlin Heidelberg New York, pp 70–77Google Scholar
  22. Dashti N, Ontko JA (1979) Rate-limiting function of 3-hydroxy-3-methylglutaryl-Coenzyme A synthetase in ketogenesis. Biochem Med 22:365–374Google Scholar
  23. Davis RA, Engelhorn SC, Pangburn SH, Weinstein DB, Steinberg D (1979) Very low density lipoprotein synthesis and secretion by cultured rat hepatocytes. J Biol Chem 254:2010–2016Google Scholar
  24. Dickson RB, Eisenfeld AJ (1979) Estrogen receptor in rat liver: Translation to the nucleus in isolated parenchymal cells. Endocrinology 105:627–635Google Scholar
  25. Duvivier J, Famerie C, Wolff D (1979) Multiple oestrogen-binding macromolecules in rat liver cytosol. Arch Int Physiol Biochim 87:406–408Google Scholar
  26. Eggleston LV, Krebs HA (1974) Regulation of the pentose phosphate cycle. Biochem J 138:425–435Google Scholar
  27. Ernster L, Navazio F (1956) The cytoplasmic distribution of isocitric dehydrogenases. Exp Cell Res 11:483–486Google Scholar
  28. Farber E (1967) Ethionine fatty liver. Adv Lipid Res 5:119–183Google Scholar
  29. Ferré P, Pégorier J-P, Williamson D, Girard J (1978) Metabolic interactions between hepatic fatty acid oxidation and gluconeogenesis in the newborn rat. Biochem Soc Trans 6:1323–1324Google Scholar
  30. Fillios LC, Kaplan R, Martin RS, Stare FJ (1958) Some aspects of the gonadal regulation of cholesterol metabolism. Am J Physiol 193:47–51Google Scholar
  31. Finkelstein MB, Auringer MP, Halper LA, Linn TC, Singh U, Srere PA (1979) Binding of specific ATP citrate lyase and fatty acid synthetase antibodies to heavy populations of rat liver polysomes. Eur J Biochem 99:209–216Google Scholar
  32. Fischer GM, Swain ML (1980) Plasma lipid levels and systolic blood pressure in ovariectomized and intact rats treated with contraceptive and other sex steroids. Artery 6:307–319Google Scholar
  33. Fitch WM, Chaikoff IL (1960) Extent and patterns of adaption of enzyme activities in livers of normal rats fed diets high in glucose and fructose. J Biol Chem 235:554–557Google Scholar
  34. Flick PK, Chen J, Alberts AW, Vagelos RP (1978) Translation of rat liver fatty acids synthetase m RNA in a cell-free system derived from wheat germ. Proc Natl Acad Sci 75:730–734Google Scholar
  35. Forsgren E (1928) Mikroskopische Untersuchungen über die Gallebildung in den Leberzellen. Z Zellforsch Mikrosk Anat 6:647–688Google Scholar
  36. Garcia DR, Holten D (1975) Inhibition of rat liver glucose-6-phosphate dehydrogenase synthesis by glucagon. J Biol Chem 250:3960–3965Google Scholar
  37. Gerok W (1973) Pathogenese, Diagnose und Therapie der Fettleber. Med Welt 24:862–867Google Scholar
  38. Gibson DH, Lyons TR, Scott DF, Muto Y (1972) Synthesis and degradation of the lipogenic enzymes of rat liver. Adv Enzyme Regul 10:187–204Google Scholar
  39. Glock GE, McLean P (1953) Further studies on the properties and assay of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem J 55:400–408Google Scholar
  40. Glock GE, McLean P (1955) A preliminary investigation of the hormonal control of the hexose monophosphate oxidative pathway. Biochem J 61:390–397Google Scholar
  41. Goebell H, Pette D (1967) Die intrazelluläre Verteilung von DPN-und TPN-spezifischer Isocitrat-Dehydrogenase. Enzymol Biol Clin 8:161–175Google Scholar
  42. Gooding PE, Chayen J, Sawyer B, Slater TF (1978) Cytochrome P-450 distribution in rat liver and the effect of sodium phenobarbitone administration. Chem-Biol Interact 20:299–310Google Scholar
  43. Goodridge AG, Adelman TG (1976) Regulation of malic enzyme synthesis by insulin, triiodoethyronine, and glucagon in liver cells in culture. J Biol Chem 251:3027–3032Google Scholar
  44. Grant AG, Billing BH (1977) The isolation and characterisation of a bile ductule cell population from normal and bile duct ligated rat livers. Br J Exp Pathol 58:301–310Google Scholar
  45. Greenbaum AL, Gumaa KA, McLean P (1971) The distribution of hepatic metabolites and the control of the pathways of carbohydrate metabolism in animals of different dietery and hormonal status. Arch Biochem Biophys 143:617–663Google Scholar
  46. Groener IEM, Klein W, Van Golde LMG (1979) The effect of fasting and refeeding on the composition and synthesis of triacylglyocerols, phosphatidylcholines and phosphatidylethanolamines in rat liver. Arch Biochem Biophys 198:287–295Google Scholar
  47. Guder WG, Habicht A, Kleiße J, Schmidt U, Wieland DH (1975) The diagnostic significance of liver cell inhomogeneity: serum enzymes in patients with central liver cell necrosis and the distribution of glutamate dehydrogenase in normal human liver. Z Klin Chem Klin Biochem 13:311–318Google Scholar
  48. Guder WG, Schmidt U, Funk B, Weis J, Pürschel S (1976) Liver cell heterogeneity. The distribution of pyruvatekinase and phosphoenolpyruvate carboxykinase (GTP) in the liver lobule of fed and starved rats. Hoppe-Seyler's Z Physiol Chem 357:1793–1800Google Scholar
  49. Gumaa KA, McLean P (1971) Factors controlling the flux of glucose through the pentose phosphate pathway. Postgrad Med J 47:403–406Google Scholar
  50. Hardonk MJ, Koudstaal J (1976) Enzyme histochemistry as a link between biochemistry and morphology. Prog Histochem Cytochem 8:1–68Google Scholar
  51. Heimberg (1980) Zit. nach Grundy SM, Kern F (1980) Workshop on regulation of hepatic cholesterol and bile acid metabolism. J Lipid Res 21:496–500Google Scholar
  52. Hildebrand R (1980) Nuclear volume and cellular metabolism. Adv Anat Embryol Cell Biol 60:1–54Google Scholar
  53. Holten D, Procsal D, Chang ML (1976) Regulation of pentose phosphate pathway dehydrogenases by NADP+/NADPH ratio. Biochem Biophys Res Commun 68:436–441Google Scholar
  54. Hori SH, Kamada T, Matsui S (1967) Electrophoretic separation of the magnesium-dependent glucose-6-phosphate dehydrogenase. J Histochem Cytochem 15:419–420Google Scholar
  55. Hori SH, Matsui S (1967) Effect of hormones on hepatic glucose-6-phosphate dehydrogenase of rat. J Histochem Cytochem 15:530–534Google Scholar
  56. Hosemann W, Teutsch HF, Sasse D (1979) Identification of G6PDH-active sinusoidal cells as Kupffer cells in the rat liver. Cell Tissue Res 196:237–247Google Scholar
  57. Hubbard DD, Allman DW, McLain GS, Gibson DM (1961) Fatty acid synthesis from malonyl CoA in liver from staryed rats refed a fat-free diet. Fed Proc 20:274Google Scholar
  58. Huggins C, Yao F (1959) Influence of hormones on liver. I. Effects of steroids and thyroxine on pyridine nucleotide-linked dehydrogenases. J Exp Med 110:899–919Google Scholar
  59. Hunter F, Hagy GW (1969) Interactions of tissue glucose-6-phosphate dehydrogenase (G6PD) activity with age and sexual development in the rat. Endokrinologie 54:85–97Google Scholar
  60. Hutchison JS, Holten D (1978) Quantitation of messenger RNA levels for rat liver 6-phosphogluconate dehydrogenase. J Biol Chem 253:52–57Google Scholar
  61. Jacobson NO (1969) The histochemical localization of lactic dehydrogenase isoenzymes in the rat nephron by means of an improved polyvinyl alcohol method. Histochemie 20:250–265Google Scholar
  62. Jungermann K, Möhler H (1980) Biochemie: Lehrbuch für Studierende der Medizin, Biologie und Pharmazie. Springer, Berlin Heidelberg New YorkGoogle Scholar
  63. Jungermann K, Katz N, Sasse D (1976) Possible zonation of rat liver parenchyma into glucogenic and glycolytic hepatocytes. In: Tager JM, Söling HD, Williamson JR (eds) Use of isolated liver cells and kidney tubules in metabolic studies. North-Holland, Amsterdam pp 404–407Google Scholar
  64. Jungermann K, Sasse D (1978) Heterogeneity of liver parenchymal cells. Trends Biochem Sci 3:198–202Google Scholar
  65. Kalina M, Gahan PB (1965) A quantitative study of the validity of the histochemical demonstration for pyridine nucleotide-linked dehydrogenases. Histochemie 5:430–436Google Scholar
  66. Kalk H (1965) Über die Fettleber. Münch Med Wochenschr 107:1141–1147Google Scholar
  67. Kauffman FC, Evans RK, Thurman RG (1977) Alterations in nicotinamide and adenine nucleotide systems during mixed-function oxidation of p-nitroanisole in perfused livers from normal and phenobarbital-treated rats. Biochem J 166:583–592Google Scholar
  68. Katz NR, Nauck NA, Wilson PT (1979) Induction of glucokinase by insulin under the permissive action of dexamethasone in primary rat hepatocyte cultures. Biochem Biophys Res Commun 88:23–29Google Scholar
  69. Katz N, Teutsch HF, Jungermann K, Sasse D (1977a) Heterogeneous reciprocal localization of fructose-1,6-bis-phosphatase and of glucokinase in microdissected periportal and perivenous rat liver tissue. FEBS Lett 83:272–276Google Scholar
  70. Katz N, Teutsch HF, Sasse D, Jungermann K (1977b) Heterogeneous distribution of glucose-6-phosphatase in micro-dissected periportal and perivenous rat liver tissue. FEBS Lett 76:226–230Google Scholar
  71. Kief H (1964) Studien zur Morphologie des Neutralfettstoffwechsel. Zwanglose Abhandlungen aus dem Gebiet der normalen und pathologischen. Anatomie 14:1–57Google Scholar
  72. Kimura H, Yamashita M (1972) Studies on microsomal G6PDH of rat liver. J Biochem (Tokyo) 71:1009–1014Google Scholar
  73. Kimura K, Endon H, Suto J, Sakai F (1979) Glucose dehydrogenase (hexose-6-phosphate dehydrogenase) and the microsomal electron transport system. J Biochem (Tokyo) 85:319–326Google Scholar
  74. Klingenberg M, Pette D (1962) Proportions of mitochondrial enzymes and pyridine nucleotides. Biochem Biophys Res Commun 7:430–432Google Scholar
  75. Kong M-S, Landau BR (1977) Compartmentation of NADPH in rat liver. Arch Biochem Biophys 180:69–74Google Scholar
  76. Kornacker MS, Ball EG (1965) Citrate cleavage in adipose tissue. Biochemistry 54:899–904Google Scholar
  77. Koudstaal J, Hardonk MJ (1969) Histochemical demonstration of enzymes related to NADPH-dependent hydroxylating systems in rat liver after phenobarbital treatment. Histochemie 20:68–77Google Scholar
  78. Koudstaal J, Hardonk MJ (1970) Histochemical demonstration of enzymes in rat liver during postnatal development. Enzymes related to NADPH-dependent hydroxylating systems and to sex difference. Histochemie 23:71–81Google Scholar
  79. Krebs HA (1966) The regulation of release of ketone bodies by the liver. Adv Enzyme Regul 4:339–354Google Scholar
  80. Krebs HA, Williamson DH, Bates MW, Page AM, Hawkins RA (1971) The role of ketone bodies in caloric homeostasis. Adv Enzyme Regul 9:387–409Google Scholar
  81. Kugler P, Wrobel K-H (1978) Studies on the optimalisation and standardisation of the light microscopical succinate dehydrogenase histochemistry. Histochemistry 57:47–60Google Scholar
  82. Lakshmanan MR, Neprokoeff CM, Porter JW (1972) Control of the synthesis of fatty acid synthetase in rat liver by insulin, glucagon, and adenosine-3′,5′-cyclic monophosphate (fat-free diet/diabetes). Proc Natl Acad Sci 69:3516–3519Google Scholar
  83. Lehninger AL (1975) Biochemistry, 2nd ed. Worth, New YorkGoogle Scholar
  84. Lehninger AL, Sudduth HC, Wise JB (1960) D-β-Hydroxybutyric-dehydrogenase of mitochondria. J Biol Chem 235:2450–2455Google Scholar
  85. Leut BA, Lee K-H, Kim K-H (1978) Regulation of rat liver acetyl-CoA carboxylase. Stimulation of phosphorylation and subsequent inactivation of liver acetyl-CoA carboxylase by cyclic-3′-5′-monophosphate and effect on the structure of the enzyme. J Biol Chem 253:8149–8156Google Scholar
  86. Lockwood EA, Bailey E, Taylor CB (1970) Factors involved in changes in hepatic lipogenesis during development of the rat. Biochem J 118:155–162Google Scholar
  87. Lojda H, Gossrau R, Schiebler TH (1976) Enzymhistochemische Methoden. Springer, Berlin Heidelberg New YorkGoogle Scholar
  88. Loud AV (1968) A quantitative stereological description of the ultrastructure of normal rat liver parenchymal cells. J Cell Biol 37:27–46Google Scholar
  89. Madvig P, Abraham S (1980) Relationship of malic enzyme activity to fatty acid synthesis and the pathway of glucose catabolism in developing rat liver. J Nutr 110:90–99Google Scholar
  90. Majerus PW, Kilburn E (1969) Acetyl coenzyme A carboxylase. The roles of synthesis and degradation in regulation of enzyme levels in rat liver. J Biol Chem 244:6254–6262Google Scholar
  91. Mandour T, Kissebah AH, Wynn V (1977) Mechanisms of oestrogen and progesteron effects on lipid and carbohydrate metabolism: alteration in the insulin: glucagon molar ratio and hepatic enzyme activity. Eur J Clin Invest 7:181–187Google Scholar
  92. Massey ED, Butler WH (1981) Zonal changes in the rat liver following an acute dose of phenobarbitone: an ultrastructural, morphometric and biochemical correlation. Chem-Biol Interact 34:31–38Google Scholar
  93. Matschinsky FM, Hintz CS, Reichlmeier K, Quistorff B, Chance B (1978) The intralobular distribution of oxidized and reduced pyridine nucleotides in the liver of normal and diabetic rats. In: Srere PA, Estabrook R (eds) Microenvironments and metabolic compartmentation. Academic Press, New York London, pp 149–166Google Scholar
  94. Matthaei C, Sasse D, Riede UN (1976) Die Fructose-induzierte “Glykogenose”. II. Histochemische Untersuchungen zum Glykogenstoffwechsel der Rattenleber nach Fructosebelastung und bei vergleichbaren Diäten. Beitr Pathol 157:56–75Google Scholar
  95. McGarry JD, Foster DW (1980) Regulation of hepatic fatty acid oxidation and ketone body production. Annu Rev Biochem 49:395–420Google Scholar
  96. McGarry JD, Meier JH, Forster DW (1973) The effects of starvation and refeeding on carbohydrate and lipid metabolism in vivo and in the perfused rat liver. J Biol Chem 248:270–278Google Scholar
  97. Meister A (1978) Current status of the γ-glutamyl cycle. In: Sies H, Wendel A (eds) Functions of glutathione in liver and kidney. Springer, Berlin Heidelberg New York, pp 43–59Google Scholar
  98. Möllinger H (1980) Histochemische und biochemische Untersuchungen zur hormonalen Induzierbarkeit des lipogenen Areals im Leberparenchym. Inaugural-Dissertation FreiburgGoogle Scholar
  99. Moldéus P, Grundin R, Vadi H, Orrenius S (1974) A study of drug metabolism linked to cytochrome P-450 in isolated rat liver cells. Eur J Biochem 46:351–360Google Scholar
  100. Morrison GR, Karl JE, Schwartz R, Shank RE (1965) The quantitative histochemistry of the normal human liver lobule. J Lab Clin Med 65:248–256Google Scholar
  101. Neprokoeff CM, Lakshmanan MR, Ners GC, Muesing A, Kleinsek A, Porter JW (1974) Coordinate control of rat liver lipogenic enzymes by insulin. Arch Biochem Biophys 162:340–344Google Scholar
  102. Neprokoeff CM, Lau H, Porter JW (1979) Identification and qualification of polysomes synthesizing rat liver fatty acid synthetase during nutritional or hormonal regulation. Int J Biochem 10:791–796Google Scholar
  103. Novikoff AB (1959) Cell heterogeneity within the hepatic lobule of the rat (staining reactions). J Histochem Cytochem 7:240–244Google Scholar
  104. Orlson JR, Fujimoto JM (1980) Demonstration of α-D-glucose-transport in the biliary tree of the rat by use of the segmented retrograde intrabiliary injection technique. Biochem Pharmacol 29:213–219Google Scholar
  105. Otway S, Robinson DS (1967) The use of a non-ionic detergent (Triton WR 1339) to determine rates of triglyceride entry into the circulation of the rat under different physiological conditions. J Physiol 190:321–332Google Scholar
  106. Pande SV, Khan PR, Venkitasubramanian TA (1964) Nicotinamide adenine dinucleotide phosphate specific dehydrogenases in relation to lipogenesis. Biochim Biophys Acta 84:239–250Google Scholar
  107. Patel US, Owen OE (1976) Lipogenesis from ketone bodies in rat brain. Biochem J 156:603–607Google Scholar
  108. Pearse AGE (1968, 1972) Histochemistry. Theoretical and applied. (3 rd ed) Vol I. Churchill, Livingstone, London (3 rd ed) vol II. Churchill, Livingstone, LondonGoogle Scholar
  109. Peavy DE, Hansen RJ (1975) Immunological titration of rat liver glucose-6-phosphate dehydrogenase from animals fed high and low carbohydrate diets. Biochem Biophys Res Commun 66:1106–1111Google Scholar
  110. Peavy DE, Hansen RJ (1976) Effects of diet on the turnover of glucose-6-phosphate dehydrogenase in rat liver. Fed Proc 35:3Google Scholar
  111. Peavy DE, Hansen RJ (1979) Influence of diet on the in vivo turnover of glucose-6-phosphat dehydrogenase in rat liver. Biochim. Biophys Acta 586:22–30Google Scholar
  112. Pence DH, Schnell RC (1979) Sex- related differences in biotransformation of aniline hydroxylase in sprague-dawley rats. Pharmacology 18:52–56Google Scholar
  113. Petcu LG, Plaut GWE (1979) Effect of inhibition of NADP-isocitrate dehydrogenase (IDH) on urea synthesis in hepatocytes. Fed Proc 38:3147Google Scholar
  114. Procsal D, Winberry L, Holten D (1976) Dietary regulation of 6-phosphogluconate dehydrogenase. J Biol Chem 251:3539–3544Google Scholar
  115. Ramsey RB (1976) Leucine and D-3-hydroxybutyrate as lipid precursors in developing rat spinal cord and liver. Biochem J 158:501–504Google Scholar
  116. Rappaport AM (1976) The microcirculatory acinar concept of normal and pathological hepatic structure. Beitr Pathol 157:215–243Google Scholar
  117. Rappaport AM, Borowy ZJ, Longheed WH, Lotto WN (1954) Subdivision of hexagonal liver lobules into a structural and functional unit; role in hepatic physiology and pathology. Anat Rec 119:11–34Google Scholar
  118. Rappaport AM, Sasse D (1979) Leberazinus: die strukturelle und fujktionelle Lebereinheit. In: Kühn HA, Wernze H (eds) Klinische Hepatologie. Thieme, Stuttgart pp 1.2–1.9Google Scholar
  119. Reith A, Schüler B, Vogell W (1968) Quantitative und qualitative elektronenmikroskopische Untersuchungen zur Struktur des Leberläppchens normaler Ratten. Z Zellforsch 89:225–240Google Scholar
  120. Rieder H, Teutsch HF, Sasse D (1978) NADP-Dependent dehydrogenases in rat liver parenchyma. I. Methodological studies on the qualitative histochemistry of G6PDH, 6PGDH, malic enzyme and ICDH. Histochemistry 56:283–298Google Scholar
  121. Robinson SU, Williamson DH (1978) Utilization of D-3-hydroxy [3-14C] butyrate for lipogenesis in vivo in lactating rat mammary gland. Biochem J 176:635–638Google Scholar
  122. Robinson AU, Williamson DH (1980) Physiological roles of ketone bodies as substrates and signals in mammalian tissues. Physiol Rev 60:143–188Google Scholar
  123. Rodrîguez-Segade A, Carrión A, Freire M (1979) Isolation and purification of a regulating cofactor of the pentose-phosphate-pathway. Biochem Biophys Res Commun 89:148–154Google Scholar
  124. Rognstad R (1980) Pathways of NADPH in rat liver. Evidence favouring a single cytosolic pool. Arch Biochem Biophys 199:140–146Google Scholar
  125. Rognstad R, Katz J (1979) Effects of 2,4-dihydroxybutyrate on lipogenesis in rat hepatocytes. J Biol Chem 254:11969–11972Google Scholar
  126. Romeis B (1968) Mikroskopische Technik. 16 Aufl. R Oldenbourg, MünchenGoogle Scholar
  127. Romsos DR, Leveille GA (1974) Effect of diet on activity of enzymes involved in fatty acid and cholesterol synthesis. Adv Lipid Res 12:97–146Google Scholar
  128. Root RK, Metcalf J, Oshino N, Chance B (1975) H2O2 release from human granulocytes during phagocytosis. I. Documentation, quantitation and some regulatory factors. J Clin Invest 33:1207–1215Google Scholar
  129. Rossi F, Zatti M (1966) Effect of phagocytosis on the carbohydrate metabolism of polymorphonuclear leucocytes. Biochim Biophys Acta 121:110–119Google Scholar
  130. Rudack D, Chisholm EM, Holten D (1971a) Rat liver glucose-6-phosphate dehydrogenase regulation by carbohydrate diet and insulin. J Biol Chem 246:1249–1254Google Scholar
  131. Rudack D, Davie B, Holten D (1971b) Regulation of rat liver glucose-6-phosphate dehydrogenase levels by adenosine cyclic-3′,5′-monophosphate. J Biol Chem 246:7823–7824Google Scholar
  132. Rudack D, Gozukara EM, Chisholm EM, Holten D (1971c) The effect of dietary carbohydrate and fat on the synthesis of rat liver 6-phosphogluconate dehydrogenase. Biochim Biophys Acta 252:305–313Google Scholar
  133. Saggerson ED, Greenbaum AL (1970) The regulation of triglyceride synthesis and fatty acid synthesis in rat epididymal adipose tissue. Effects of altered dietary and hormonal conditions. Biochem J 119:221–242Google Scholar
  134. Sapag-Hagar U, Lagunas R, Sols A (1973) Apparent unbalance between the acitivities of 6-phosphogluconate and glucose-6-phosphate dehydrogenases in rat liver. Biochem Biophys Res Commun 50:179–185Google Scholar
  135. Sasse D, Katz N, Jungermann K (1975) Functional heterogeneity of rat liver parenchyma and isolated hepatocytes. FEBS Lett 57:83–88Google Scholar
  136. Schlunk FF, Lombardi B (1967) On the ethionine induced fatty liver in male and female rats. Lab Invest 17:299–307Google Scholar
  137. Scholz R, Hansen W, Thurman R (1973) Interaction of mixed-function oxidation with biosynthetic processes. I. Inhibition of gluconeogenesis by aminopoyrine in perfused rat liver. Eur J Biochem 38:64–72Google Scholar
  138. Schudt C (1979) Hormonal regulation of glucokinase in primary cultures of rat hepatocytes. Eur J Biochem 98:77–82Google Scholar
  139. Severson AR, Hubbard DD, Gibson DM (1973) Changes in distribution of lipid, glucose-6-phosphate dehydrogenase and malate enzyme within the liver lobule of the rat during adaptive hyperlipogenesis. Anat Rec 175:231–242Google Scholar
  140. Shah S, Pearson DJ (1978) The effect of phenobarbitone on cytoplasmic NADP-linked dehydrogenase activities in rat liver. Biochim Biophys Acta 539:12–18Google Scholar
  141. Shank RE, Morrison G, Cheng CH, Karl I, Schwartz R (1959) Cell heterogeneity within the hepatic lobule (quantitative histochemistry). J Histochem Cytochem 7:237–239Google Scholar
  142. Shrago E, Lardy HA, Nordlie RC, Forster DO (1963) Metabolic and hormonal control of phosphoenolpyruvate carboxykinase and malic enzyme in rat liver. J Biol Chem 238:3188–3192Google Scholar
  143. Sies H, Akerboom T, Tager J (1977) Mitochondrial and cytosolic NADPH-systems and isocitrate dehydrogenase indicator metabolites during ureogenesis from ammonia in isolated rat hepatocytes. Eur J Biochem 72:301–307Google Scholar
  144. Sies H, Brauser B (1970) Interaction of mixed function oxidase with its substrates and associated redox transition of cytochrome P-450 and pyridine nucleotides in perfused rat liver. Eur J Biochem 15:531–540Google Scholar
  145. Sies H, Summer KH, Bücher Th (1975) A process requiring mitochondrial NADPH: urea formation from ammonia. FEBS Lett 54:274–278Google Scholar
  146. Sies H, Wahlländer A, Waydhas C (1978a) Properties of glutathione disulfide (GSSG) and glutathione-S-conjugate release from perfused rat liver. In: Sies H, Wendel A (eds) Function of glutathione in liver and kidney. Springer, Berlin Heidelberg New York, pp 120–126Google Scholar
  147. Sies H, Weigl K, Waydhas C (1978b) Metabolic consequences of drug oxidations in perfused liver and in isolated hepatocytes from phenobarbital-treated rats. In: Estabrook RW, Lindenlaub E (eds) The induction of drug metabolism. Schattauer, Stuttgart New York, pp 38–400Google Scholar
  148. Sleyster EC, Westerhuis FG, Knook DL (1977) The purification of nonparenchymal liver cell classes by centrifugal elutriation. In: Wisse E, Knook DL (eds) Kupffer cells and other liver sinusoidal cells. Biomedical Press, Amsterdam, New York, Oxford, pp 289–298Google Scholar
  149. Smith T, Loveridge N, Wills ED, Chayen J (1979) The distribution of glutathione in the rat liver lobule. Biochem J 182:103–108Google Scholar
  150. smith CM, Plaut GWE (1979) Activities of NAD-specific and NADP-specific isocitrate dehydrogenase in rat-liver mitochondria. Eur J Biochem 97:283–295Google Scholar
  151. Soler-Argilaga C, Heimberg M (1976) Comparison of metabolism of free fatty acid by isolated perfused livers from male and female rats. J Lipid Res 17:605–615Google Scholar
  152. Spence JT, Pitot HC (1979) Hormonal regulation of glucokinase in primary cultures of adult rat hepatocytes. J Biol Chem 254:12331–12336Google Scholar
  153. Spence JT, Pitot HC, Zalitis G (1979) Regulation of ATP-citrate lyase in primary cultures of adult rat hepatocytes. J Biol Chem 254:12169–12173Google Scholar
  154. Srere AP (1972) The citrate enzymes, their structures, mechanisms and biological functions. Curr Top Cell Regul 5:229–283Google Scholar
  155. Stark MJ, Frenkel R (1974) Dietary induction of hepatic matic enzyme activity, differentiation of the induction process. Life Sci 14:1563–1575Google Scholar
  156. Stary Z (1956) Physiologische Chemie einzelner Lebensvorgänge und Organe. IV. 1. Leber und Galle. In: Flaschenträger B, Lehnartz E (eds) Physiologische Chemie, ein Lehr- und Handbuch, Bd II/2a. Springer, Berlin Göttingen Heidelberg, p 139Google Scholar
  157. Storey JM, Bailey E (1978) Effect of streptozotocin diabetes and insulin administration on some liver enzyme activities in teh post-weaning rat. Enzyme 23:382–387Google Scholar
  158. Suzuki K (1975) Quantitative enzyme histochemistry of normal and injured livers. Part 1: Ultramicrochemical and histochemical discrepancies in G6PD and JCD activity within the hepatic lobule of CCl4-injured liver of rats. Acta Histochem. Cytochem. (Kyoto) 8:193–201Google Scholar
  159. Szepesi B, Vegors R, Michaelis DE, Demogy JH (1975) Long term effects of starvation-refeeding in the rat. Nutr Metab 19:45–54Google Scholar
  160. Taira Y, Greenspan P, Kaphe GF, Redick JA, Baron J (1980) Effects of phenobarbitone, pregnenolone-16-carbonitril, and 3-methylcholantren pretreatments on the distribution of NADPH-cytochrome c (P-450) reductase within the liver lobule. Mol Pharmacol 18:304–312Google Scholar
  161. Tanahaschi K, Hori SH (1979) Immunohistochemical localisation of hexose-6-phosphate dehydrogenase in various organs of the rat. Acta Histochem Cytochem (Kyoto) 12:545Google Scholar
  162. Taylor CB, Bailey E, Bartley W (1967) Changes in hepatic lipogenesis during development of the rat. Biochem J 105:715–722Google Scholar
  163. Taylor NA, Holten D (1979) Transcriptional regulation of rat liver glucose-6-phosphate dehydrogenase synthesis by cAMP. Fed Proc 38:29Google Scholar
  164. Tepperman J, Tepperman HM (1958a) Effects of antecedent food intake pattern of hepatic lipogenesis. Am J Physiol 193:55–64Google Scholar
  165. Tepperman HM, Tepperman J (1958b) The hexose monophosphate shunt and adaptive hyperlipogenesis. Diabetes 7:478–485Google Scholar
  166. Tepperman HM, Tepperman J (1963) On the response of hepatic glucose-6-phosphate dehydrogenase activity to changes in diet composition and food intake. Adv Enzyme Regul 1:121–136Google Scholar
  167. Tepperman HM, Tepperman J (1964) Patterns of dietary and hormonal induction of certain NADP-linked liver enzymes. Am J Physiol 206:357–361Google Scholar
  168. Teutsch HF (1978) Quantitative determination of G6Pase activity in histochemically defined zones of the liver acinus. Histochemistry 58:281–288Google Scholar
  169. Teutsch HF (1981) Chemomorphology of liver parenchyma. Qualitative histochemical distribution patterns and quantitative sinusoidal profiles of G6Pase, G6PDH and malic enzyme activity and of glycogen content. Prog Histochem Cytochem 14/3:1–92Google Scholar
  170. Teutsch HF, Rieder H (1979) NADP-dependent dehydrogenases in rat liver parenchyma. II. Comparison of qualitative and quantitative G6PDH distribution patterns with particular reference to sex differences. Histochemistry 60:43–52Google Scholar
  171. Thurman RG, Scholz R (1969) Mixed function oxidation in perfused rat liver. The effect of aminopyrine on oxygen uptake. Eur J Biochem 10:459–467Google Scholar
  172. Thurman RG, Scholz R (1973) Interaction of mixed-function oxidation with biosynthetic processes. 2. Inhibition of lipogenesis by aminopyrine in perfused rat liver. Eur J Biochem 38:73–78Google Scholar
  173. Towle HC, Mariash CN, Oppenheimer JH (1980) Changes in the hepatic levels of messenger ribonucleic acid for malic enzyme during induction by thyroid hormone or diet. Biochemistry 19:579–585Google Scholar
  174. Trus M, Zawalich K, Gaynor D, Matschinsky F (1980) Hexokinase and glucokinase distribution in the liver lobule. J Histochem Cytochem 28:579–581Google Scholar
  175. Tsai AC, Dyer JA (1973) Influence of dietary cholesterol and cholic acid on liver carbohydrate metabolism enzymes in rats. J Nutr 103:93–101Google Scholar
  176. Veech RL, Guynn RW (1974) The control of hepatic fatty acid synthesis in vivo. In: Lundquist F, Typstrup N (eds) Regulation of hepatic metabolism. Munksgaard, Copenhagen, pp 337–357Google Scholar
  177. Volpe JJ, Marasa JC (1975) Hormonal regulations of fatty acid synthetase, acetyl-CoA-carboxylase and fatty acid synthesis in mammalian adipose tissue and liver. Biochim Biophys Acta 380:454–472Google Scholar
  178. Wahn V, Scheer G, Langhans W, Schimassek, H (1980) Glycolyse in spezialisierten Leber-Parenchymzellen? Eur J Cell Biol 21:72–78Google Scholar
  179. Walli RA (1978) Interrelation of aerobic glycolysis and lipogenesis in isolated perfused liver of well fed rats. Biochim Biophys Acta 539:62–80Google Scholar
  180. Watanabe A, Takesue A, Taketa K (1975) Prevention of dietary induction of rat liver glucose-6-phosphate dehydrogenase by cyclic adenosine-3,5-monophosphate and its elimination by glucose prefeeding. Enzyme 21:436–447Google Scholar
  181. Weigl K, Sies H (1977) Drug oxidations dependent on cytochrome P-450 in isolated hepatocytes. The role of the tricarboxylates and the aminotransferases in NADPH supply. Eur J Biochem 77:401–408Google Scholar
  182. Weinstein J, Forte LR, Werner HV, Heimberg M (1979a) Decreased cAMP responsiveness to glucagon in livers from ethynyl estradiol treated rats. Biochem Biophys Res Commun 86:454–459Google Scholar
  183. Weinstein J, Soler-Argilaga C, Werner HV, Heimberg M (1979b) Effects of ethynyloestradiol on the metabolism of 1-14C oleate by perfused livers and hepatocytes from female rats. Biochem J 180:265–271Google Scholar
  184. Weinstein I, Turner FC, Soler-Argilaga C, Heimberg M (1978) Effects of ethinyl estradiol on serum lipoprotein lipids in male and female rate. Biochim Biophys Acta 530:394–401Google Scholar
  185. Welsh FA (1972) Changes in distribution of enzymes within the liver lobule during adaptive increases. J Histochem Cytochem 20:107–111Google Scholar
  186. Wilkinson MG, Szepesi B (1978) In vitro assay of mRNA specific for liver glucose-6-phosphate dehydrogenase (G6PD) in rats under various dietary treatments. Fed Proc 37:3055Google Scholar
  187. Williamson DH (1979) Recent developments in ketone body metabolism. Biochemical Review. Biochem Soc Trans 7:1313–1321Google Scholar
  188. Williamson DH, Bates MW, Krebs HA (1968) Activity and intracellular distribution of enzymes of ketone body metabolism. Biochem J 108:353–361Google Scholar
  189. Williamson DH, Bates MW, Page MA, Krebs HA (1971) Activities of enzymes involved in acetoacetate utilization in adult mammalian tissues. Biochem J 121:41–47Google Scholar
  190. Williamson DH, Lund P, Krebs HA (1967) The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochem J 103:514–527Google Scholar
  191. Williamson DH, Whitelaw E (1978) Physiological aspects of the regulation of ketogenesis. Biochem Soc Symp 43:137–161Google Scholar
  192. Wimmer M, Pette D (1979) Microphotometric studies on intraacinar enzyme distribution in rat liver. Histochemistry 64:23–33Google Scholar
  193. Wise EM, Ball EG (1964) Malic enzyme and lipogenesis. Proc Natl Acad Sci 52:1255–1263Google Scholar
  194. Witters LA, Moriarity D, Martin DB (1979) Regulation of hepatic acetyl coenzyme A carboxylase by insulin and glucagon. J Biol Chem 254:6644–6649Google Scholar
  195. Woods HF, Krebs HA (1971) Lactate production in the perfused rat liver. Biochem J 125:129–139Google Scholar
  196. Young JW, Shrage E, Lardy HA (1964) Metabolic controls of enzymes involved in lipogenesis and gluconeogenesis. Biochemistry 3:1687–1692Google Scholar
  197. Yugari Y, Matsuda T (1967) Glucose-6-phosphate dehydrogenase from rat liver. II. Effect of diet on enzyme activity in vivo and inhibition by long-chain-fatty acid in vitro. J Biochem (Tokyo) 61:541–549Google Scholar
  198. Zammit VA, Beis A, Newsholme EA (1979) The role of 3-oxo acid-CoA transferase in the regulation of ketogenesis in the liver. FEBS Lett 103:212–215Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • H. Rieder
    • 1
  1. 1.Institute of AnatomyUniversity of Freiburg/Br.Germany

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