Skip to main content
Log in

The enzyme cytochemistry of the intracellular organelles in the rat choroid plexus epithelial cell

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

Electron microscopic cytochemical studies on the rat choroid plexus epithelium have revealed enzymatic sites for the activities of acid phosphatase, glucose-6-phosphatase and thiamine pyrophosphatase on different organelles. Only the activity of acid phosphatase has been previously described. Acid phosphatase, glucose-6-phosphatase and thiamine pyrophosphatase were respectively situated mainly in the lysosomes, in the endoplasmic reticulum and nuclear envelope, and in the Golgi complex. These three enzymes can thus be considered as marker enzymes for their respective organelles in the choroid plexus epithelial cells as well as in other tissue cells. The possible function of these enzymes in the choroid plexus epithelial cells is also briefly discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anderson PJ, Song SK (1962) Acid phosphatase in the nervous system. J Neuropathol Exp Neurol 21:263–283

    Google Scholar 

  • Bartoniček V, Lojda Z (1964) Topochemistry of enzymes of choroid plexus and ependyma of four animal species. Acta Histochem 19:357–368

    Google Scholar 

  • Becker NH, Goldfischer J, Shin W, Novikoff AB (1960) The localization of enzyme activities in the rat brain. J Biophys Biochem Cytol 8:649–663

    Google Scholar 

  • Gomori G (1941) The distribution of phosphatase in normal organs and tissues. J Cell Comp Physiol 17:71–83

    Google Scholar 

  • Gomori G (1950) An improved histochemical technique for acid phosphatase. Stain Technol 25:81–85

    Google Scholar 

  • Ishii S, Matano S (1968) Electron microscopic observations on phosphatase activities at the choroid plexus in rat brain. II. On the structural localization of β-glycerophosphatase at the alkali pH. Okajimas Folia Anat Jpn 44:149–157

    Google Scholar 

  • Kaluza JS, Burstone MS, Klatzo I (1964) Enzyme histochemistry of the chick choroid plexus. Acta Neuropathol 3:480–489

    Google Scholar 

  • Leduc EH, Wislocki GB (1952) The histochemical localization of acid and alkaline phosphatases, nonspecific estrase and succinic dehydrogenase in the structures comprising the hematocephalic barrier of the rat. J Comp Neurol 97:241–266

    Google Scholar 

  • Manocha SL, Shantha TR, Borne GH (1967a) Histochemical mapping of the distribution of monoamine oxidase in the diencephalon and basal telencephalic centers of the brain of squirrel monkey (Saimiri sciureus). Brain Res 6:570–586

    Google Scholar 

  • Manocha SL (1970) Histochemical distribution of alkaline and acid phosphatase and adenosine triphosphatase in the brain of squirrel monkey. Histochemie 21:221–235

    Google Scholar 

  • Masuzawa T, Saito T, Saito F (1980) Cytochemical study of the electron microscopical localization of K+-dependent p-nitrophenylphosphatase activity on choroidal ependymal epithelium in normal rat brain comparing with the activity of Mg2+-ATPase and alkaline phosphatase. Acta Histochem Cytochem 13:394–403

    Google Scholar 

  • Matano S, Ishii S (1967) Electron microscopic observations on phosphatase activities at the choroid plexus in rat brain. I. On the structural localization of β-glycerophosphatase at the acid pH. Okajima Folia Anat Jpn 44:1–9

    Google Scholar 

  • Matano S, Ishii S (1973) The fine structural localization of Na−K ATPase in the choroid plexus. J Electron Microsc (Tokyo) 22:375–359

    Google Scholar 

  • Milhorat TH, Davis DA, Hammock MK (1975) Localization of ouabain-sensitive Na−K-ATPase in frog, rabbit and rat choroid plexus. Brain Res 99:170–174

    Google Scholar 

  • Naidoo D, Pratt OE (1951) The localization of some acid phosphatases in brain tissue. J Neurol Neurosurg Psychiatry 14:287–294

    Google Scholar 

  • Novikoff AB, Goldfischer S (1961) Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytological studies. Proc Natl Acad Sci USA 47:802–810

    Google Scholar 

  • Pearse AGE (1972) Method for glucose-6-phosphatase. In: Histochemistry. Theoretical and applied, vol 1, 3rd ed. Churchill Livingstone, Edinburgh and London, p 535

    Google Scholar 

  • Saito T, Ogawa K (1966) Electron cytochemical examination of the thiamine pyrophosphatase activity in the intestine of the rat. Arch Histol Jpn 27:473–484

    Google Scholar 

  • Saito T, Ogawa K (1967) Ultracytochemical changes of the glucose-6-phosphatase (D-glucose-6-phosphate phosphohydrolase) activity in liver cells of the rat treated with phenobarbital. Okajima Folia Anat Jpn 44:11–27

    Google Scholar 

  • Shantha TR, Manocha SL (1968) Enzyme histochemistry of the choroid plexus in rat and squirrel monkey. Histochemie 14:149–160

    Google Scholar 

  • Shimizu N (1950) Histochemical studies on the phosphatase of the nervous system. J Comp Neurol 93:201–217

    Google Scholar 

  • Tice LW, Barrnett RJ (1962) The fine structural localization of glucose-6-phosphatase in rat liver. J Histochem Cytochem 10:754–762

    Google Scholar 

  • Torack RM, Besen M, Becker NH (1967) Localization of adenosinetriphosphatase in capillaries of the brain as revealed by electron microscopy. Neurology (NY) 11:71–76

    Google Scholar 

  • Torack RM, Barrnett RJ (1964) The fine structural localization of nucleoside phosphatase activity in the blood brain barrier. J Neuropathol Exp Neurol 23:46–59

    Google Scholar 

  • Wachstein M, Meisel EM (1956) On the histochemical demonstration of glucose-6-phosphatase. J Histochem Cytochem 4:592

    Google Scholar 

  • Wislocki GB, Dempsey EW (1948) The chemical cytology of the choroid plexus and blood brain barrier of the rhesus monkey (Macaca mulatta). J Comp Neurol 88:319–346

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Masuzawa, T., Shimabukuro, H., Sato, F. et al. The enzyme cytochemistry of the intracellular organelles in the rat choroid plexus epithelial cell. Histochemistry 72, 489–498 (1981). https://doi.org/10.1007/BF00493270

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00493270

Keywords

Navigation