Summary
Compact nucleoli without the segregation of nucleolar components were produced in hepatocytes by the treatment of experimental rats with cycloheximide to facilitate a cytochemical study on the organization of nucleolar components in such nucleoli. The extraction of pepsin pretreated specimens with nucleases (deoxyribonuclease and ribonuclease) demonstrated that compact nucleoli are characterized by a relatively uniform distribution of RNP components which mask a microtrabecular intranucleolar network. This network apparently consists of proteins and contains fine DNA filaments.
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Smetana, K., Daskal, I. & Busch, H. Cytochemistry of the microtrabecular network in compact nucleoli of hepatocytes treated with cycloheximide. Histochemistry 65, 301–308 (1980). https://doi.org/10.1007/BF00493179
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DOI: https://doi.org/10.1007/BF00493179