Summary
Soybean agglutinin (SBA) has been localized in Glycine max (soybean) var. Altona at the ultrastructural level by the gold method. SBA was detected by marking thin sections of different part of the seed with gold granules (12 nm in size) labelled with anti-SBA antiserum. Upon examination by transmission electron microscopy, the lectin was found uniformly distributed in most of the protein bodies of the cotyledon. SBA was also present in the embryo axis.
References
Bal, A.K., Verma, D.P.S., Byrne, H., Maclachlan, G.A.: Subcellular localization of cellulases in auxin-treated pea. J. Cell Biol. 69, 97–105 (1976)
Batten, T.F.C., Hopkins, C.R.: Use of protein A-coated colloidal gold particles for immunoelectron-microscopic localization of ACTH on ultrathin sections. Histochemistry 60, 317–320 (1979)
Baumgartner, B., Tokuyasu, K.T., Chrispeels, M.J.: Localization of ricilin peptidohydrolase in the cotyledons of mung bean seedlings by immuno-fluorescence microscopy. J. Cell Biol. 79, 10–19 (1978)
Clarke, A.E., Knox, R.B., Jermyn, M.A.: Localization of lectins in legume cotyledons. J. Cell Biol. 19, 157–167 (1975)
Goldstein, I.J., Hayes, E.C.: The lectins: carbohydrate-binding proteins of plants and animals. Adv. Carbohydr. Chem. Biochem. 35, 127–340 (1978)
Horejsi, V.: Galactose oxidase. An enzyme with lectin properties. Biochim. Biophys. Acta 577, 383–388 (1979)
Horisberger, M.: Evaluation of colloidal gold as a cytochemical marker for transmission and scanning electron microscopy. Biol. Cell. (1979, in press)
Horisberger, M., Rosset, J.: Colloidal gold, a useful marker for transmission and scanning electron microscopy. J. Histochem. Cytochem. 25, 295–305 (1977)
Horisberger, M., Vonlanthen, M.: Location of mannan and chitin on thin sections of budding yeasts with gold markers. Arch. Microbiol. 115, 1–7 (1977)
Horisberger, M., Vonlanthen, M.: Simultaneous localization of an hepatic binding protein specific for galactose and of galactose-containing receptors on rat hepatocytes. J. Histochem. Cytochem. 26, 960–966 (1978)
Horisberger, M., Farr, D.R., Vonlanthen, M.: Ultrastructural localization of β-D-galactan in the nuclei of the myxomycete Physarum polycephalum. Biochim. Biophys. Acta 542, 308–314 (1978a)
Horisberger, M., Vonlanthen, J., Rosset, J.: Localization of α-galactomannan and of wheat germ agglutinin receptors in Schizosaccharomyces pombe. Arch. Microbiol. 119, 107–111 (1978b)
Hwang, D.L., Yang, W.K., Foard, D.E., Lin, K.T.: Rapid release of protease inhibitors form soybeans. Immunochemical quantitation and parallels with lectins. Plant Physiol. 61, 30–34 (1978)
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137–138A (1965)
Mialonier, G., Privat, J.P., Monsigny, M., Kahlem, G., Durand, R.: Isolement, propriétés physicochimiques et localisation in vivo d'une phytohémagglutine (lectine) de Phaseolus vulgaris L. (Var. rouge). Physiol. Vég. 11, 519–537 (1973)
Pernollet, J.C.: Protein bodies of seeds: Ultrastructure, biochemistry, biosynthesis and degradation. Phytochemistry 17, 1473–1480 (1978)
Pueppke, S.G., Bauer, W.D., Keegstra, K., Ferguson, A.T.: Role of lectins in plant microorganism interactions. Plant Physiol. 61, 779–784 (1978)
Shumway, L.K., Yang, V.V., Ryan, C.A.: Evidence for the presence of proteinase inhibitor I in vacuolar protein bodies of plant cells. Planta 129, 161–165 (1976)
Spurr, A.R.: A low viscosity epoxy resin embedding medium for electrom microscopy. J. Ultrastruct. Res. 26, 31–43 (1969)
Stathis, E.C., Fabrikanos, A.: Preparation of colloidal gold. Chem. Ind. (London) 27, 860–861 (1958)
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Horisberger, M., Vonlanthen, M. Ultrastructural localization of soybean agglutinin on thin sections of Glycine max (soybean) var. Altona by the gold method. Histochemistry 65, 181–186 (1980). https://doi.org/10.1007/BF00493167
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DOI: https://doi.org/10.1007/BF00493167