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Histochemistry

, Volume 65, Issue 2, pp 167–171 | Cite as

PAS-positive reaction of phenolic inclusions in plant cell vacuoles

  • T. Geier
Article

Summary

The experimental data presented show that positive reaction of phenolic inclusions in the PAS test may be caused by the presence of phenolic glycosides and/or certain non-glycosidic phenols, namely flavan-3,4-diols. These both possibilities and their respective importance under conditions of conventional histological procedures are discussed. The findings emphasize the need for extreme care in interpreting the results of the PAS reaction applied to plant material.

Keywords

Public Health Experimental Data Phenol Plant Cell Plant Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bate-Smith, E.C., Lerner, N.H.: Leuco-anthocyanins. II. Systematic distribution of leuco-anthocyanins in leaves. Biochem. J. 58, 126–132 (1954)Google Scholar
  2. Considine, J.A., Knox, R.B.: Development and histochemistry of the pistil of the grape, Vitis vinifera. Ann. Bot. 43, 11–22 (1979)Google Scholar
  3. Endress, A.G., Thomson, W.W.: Ultrastructural and cytochemical studies on the developing adhesive disc of Boston Ivy tendrils. Protoplasma 88, 315–331 (1976)Google Scholar
  4. Feder, N., O'Brien, T.P.: Plant microtechnique: Some principles and new methods. Am. J. Bot. 55, 123–142 (1968)Google Scholar
  5. Geier, T.: Dedifferentiation of tannin cells in excised leaf fragments of Cyclamen persicum Mill. cultured in vitro. Z. Pflanzenphysiol. 78, 466–471 (1976)Google Scholar
  6. Ginzburg, C.: The relation of tannins to the differentiation of the root tissues in Reaumuria palaestina. Bot. Gaz. 128, 1–10 (1967)Google Scholar
  7. Harborne, J.B.: Phenolic glycosides and their distribution. In: Biochemistry of phenolic compounds, Harborne, J.B., (ed.). pp. 129–169. New York: Academic Press 1964Google Scholar
  8. Harborne, J.B.: Phytochemical methods. London: Chapman and Hall 1973Google Scholar
  9. Hawker, J.S., Buttrose, M.S., Soeffky, A., Possingham, J.V.: A simple method for demonstrating macroscopically the location of polyphenolic compounds in grape berries. Vitis 11, 189–192 (1972)Google Scholar
  10. Hotchkiss, R.D.: A microchemical reaction resulting in the staining of polysaccharide structures in fixed tissue preparations. Arch. Biochem. 16, 131–141 (1948)Google Scholar
  11. Ling-Lee, M., Chilvers, G.A., Ashford, A.E.: A histochemical study of phenolic materials in mycorrhizal and uninfected roots of Eucalyptus fastigiata Deane and Maiden. New Phytol 78, 313–328 (1977)Google Scholar
  12. McCully, M.E.: Histological studies on the genus Fucus. I. Light microscopy of the mature vegetative plant. Protoplasma 62, 287–305 (1966)Google Scholar
  13. Mc Manus, J.F.A.: Histological and histochemical use of periodic acid. Stain Technol. 23, 99–108 (1948)Google Scholar
  14. Mueller, W.C., Greenwood, A.D.: The ultrastructure of phenolic-storing cells fixed with caffeine. J. Exp. Bot. 29, 757–764 (1978)Google Scholar
  15. Peterson, R.L., Kott, L.S.: The sorus of Polypodium virginianum: Some aspects of the development and structure of paraphyses and sporangia. Can. J. Bot. 52, 2283–2288 (1974)Google Scholar
  16. ribéreau-Gayon, P.: Plant Phenolics. Edinburgh, Oliver & Boyd 1972Google Scholar
  17. Sklarz, B.: Organic chemistry of periodates. Q. Rev. Chem. Soc. 21, 3–28 (1967)Google Scholar
  18. Swift, J.G., Buttrose, M.S., Possingham, J.V.: Stomata and starch in grape berries. Vitis 12, 38–45 (1973)Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • T. Geier
    • 1
  1. 1.Institut für Botanik der Forschungsanstalt für Weinbau, Gartenbau, Getränketechnologie und LandespflegeGeisenheimGermany

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