, Volume 89, Issue 3, pp 277–282 | Cite as

Light and electron microscopical postembedding lectin histochemistry for WGA-binding sites in the renal cortex of the mouse embedded in polyhydroxy aromatic resins LR-White and LR-Gold

  • R. Herken
  • M. Fussek
  • M. Thies


This work describes a technique which permits study of the postembedding lectin histochemistry for WGA-binding sites at the light and electron microscopical level on the same resin embedded tissue without removing or etching of the resin. Unfixed kidney pieces or kidney pieces fixed in 4% formaldehyde were embedded in the hydrophilic polyhydroxy aromatic resins LR-Gold and LR-White, following dehydration in up to 70% ethanol, 90% ethanol or 100% ethanol. LR-Gold was cryopolymerised at −25° C using the light sensitive initiator benzil, whereas LR-White was heat-cured at +50° C. The localisation of WGA-binding sites at the light microscopical level was investigated using FITC-labelled WGA. The ultrastructural localisation of WGA-binding sites was performed using 15 nm gold-labelled WGA. The best fluorescence staining results were obtained on fixed or unfixed tissue dehydrated in up to 70% ethanol and embedded in LR-Gold. At the ultrastructural level, the best staining results for WGA-binding sites were seen on tissue sections, dehydrated in up to 90% ethanol prior to embedding in LR-Gold.


Formaldehyde Tissue Section Microscopical Level Renal Cortex Fluorescence Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bretton R, Bariety J (1976) A comparative ultrastructural localisation of concanavalin A, wheat germ and ricinus communis on glomeruli of normal rat kidney. J Histochem Cytochem 24:1093–1100Google Scholar
  2. Ellinger A, Pavelka M (1985) Post-embedding localisation of glycoconjugates by means of lectins on thin sections of tissue embedded in LR-White. Histochem J 17:1321–1335Google Scholar
  3. Farnum CE, Wilsman NJ (1984) Lectin-binding histochemistry of non-decalcified growth plate cartilage: A postembedment method for light microscopy of Epon-embedded tissue. J Histochem Cytochem 32:593–607Google Scholar
  4. Holthöfer H (1983) Lectin binding sites in kidney: A comparative study of 14 animal species. J Histochem Cytochem 31:531–537Google Scholar
  5. Holthöfer H, Virtanen I, Pettersson E, Törnroth T, Alfthan O, Linder E, Miettinen A (1981) Lectins as fluorescence microscopic markers for saccharides in the human kidney. Lab Invest 45:391–399Google Scholar
  6. Jones CJP, Stoddard RW (1986) A postembedding avidin-biotin peroxidase system to demonstrate the light and electron microscopical localization of lectin binding sites in rat kidney tubules. Histochem J 18:371–379Google Scholar
  7. Lucocq JM, Roth J (1984) Demonstration of antigenic and lectinbinding sites in semithin resin sections. J Histochem Cytochem 32:1075–1083Google Scholar
  8. Newman GR (1987) Use and abuse of LR-White. Histochem J 19:118–120Google Scholar
  9. Newman GR, Jasani B, Williams ED (1983) A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope. Histochem J 15:543–555Google Scholar
  10. Roth J (1983) Application of immunocolloids in light microscopy. Demonstration of lectin-binding sites in paraffin sections by the use of lectin-gold or glycoprotein-gold complexes. J Histochem Cytochem 31:547–552Google Scholar
  11. Roth J, Brown D, Orci L (1983) Regional distribution of N-acetyl-d-galactosamine residues in the glycocalyx of glomerular podocytes. J Cell Biol 96:1189–1196Google Scholar
  12. Roth J, Lucocq M, Charest PM (1984) Light and electron microscopic demonstration of sialic acid residues with the lectin from limax flavus: A cytochemical affinity technique with the use of fetuin-gold complexes. J Histochem Cytochem 32:1167–1179Google Scholar
  13. Yokota S, Oda T (1985) Immunocytochemical demonstration of serine: Pyruvate aminotransferase in peroxisomes and mitochondria of rat kidney. Histochemistry 83:81–85Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • R. Herken
    • 1
  • M. Fussek
    • 1
  • M. Thies
    • 1
  1. 1.Abteilung HistologieZentrum AnatomieGöttingenFederal Republic of Germany

Personalised recommendations