Histochemistry

, Volume 91, Issue 4, pp 283–289 | Cite as

A comparative study of PAS-phosphotungstic acid-Diamine Supra Blue FGL and immunological reactions for type I collagen

  • R. N. Rao
  • P. M. Fallman
  • D. Greer Falls
  • S. N. Meloan
Article

Summary

Immunohistochemical techniques proved valuable in histological studies of various types of collagens. However drawbacks include non-specific reactions of antibodies, masking of antigens, and the high cost of antibodies. This study was undertaken to ascertain the specificity of the PAS-phosphotungstic acid-Diamine Supra Blue FGL (PAS PTA-DSB-FGL) reaction for type I collagen, differentiating it from other collagens. Duplicate series of methaearn-fixed sections of various tissues were treated with the PAS-PTA-DSB FGL reaction and the peroxidase-antiperoxidase (PAP) technique for type I collagen and the staining patterns were compared. Fibers binding the blue dye were found only at sites reacting with antibodies against type I collagen. These observations indicate that the PAS-PTA-DSB FGL procedure is suitable for visualization of type I collagen, e.g. in screening of large series of sections and in the practice of surgical and autopsy pathology.

Keywords

Public Health Collagen Large Series Staining Pattern Histological Study 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adachi E, Hayashi T (1986) In vitro formation of hybrid fibrils of type V collagen and type I collagen. Limited growth of type I collagen into thick fibrils by type V collagen. Connect Tissue Res 14:257–266Google Scholar
  2. Altmannsberger M, Osborn M, Schauer A, Weber K (1981) Antibodies to different intermediate filament proteins. Lab Invest 45:427–434Google Scholar
  3. Bartholomew JS, Anderson JC (1983) Investigation of relationship between collagen, elastin and proteoglycans in bovine thoracic aorta by immunofluorescence technique. Histochem J 15:1177–1190Google Scholar
  4. Barton BP, Hobbs JL, Waldrop FS, Meloan SN, Puchtler H (1984) Effects of fixation on PAP reactions for prekeratin: formalin versus methacarn. Acta Anat 120:10Google Scholar
  5. Becker V, Nowack H, Gay S, Timpl R (1976) Production and specificity of antibodies against the aminoterminal region in type III collagen. Immunology 31:57–65Google Scholar
  6. Biempica L, Morecki RR, Wu CH, Giambrone M, Rojkind M (1980) Immunocytochemical localization of type B collagen. Am J Pathol 98:591–602Google Scholar
  7. Billroth T (1861) Zur normalen und pathologischen Anatomie der menschlichen Milz. Virchows Arch 20:409–425Google Scholar
  8. Chung E, Keele EM, Miller EJ (1974) Isolation and characterization of the cyanogen bromide peptides from the α1(III) chain of human collagen. Biochemistry 13:3459–3464Google Scholar
  9. Colour Index, 3rd edn, vol 4 (1971) The society of dyers and colourists and the American association of textile chemists and colorists. Lund Humphries, BradfordGoogle Scholar
  10. D'Ardenne AJ, Burns J, Sykes AC, Kirkpatrick P (1983) Comparative distribution of fibronectin and type III collagen in normal tissue. J Pathol 98:591–602Google Scholar
  11. Dean JG, Kay H, Larson ML (1959) Molybdenum chelates, estert, and organometallics. Adv Chem Ser 23:357–367Google Scholar
  12. Ewald A (1890) Zur Histologie und Chemie der elastischen Fasern und des Bindegewebes. Z Biol 26:1–56Google Scholar
  13. Fietzek PP, Kühn K (1976) The primary structure of collagen. Int Rev Connect Tissue Res 7:1–60Google Scholar
  14. Furthmayr H (1982) Immunization procedures, isolation by affinity chromatography and serological and immunological characterization of collagen specific antibodies. In: Furthmayr H (ed) Immunochemistry of the extracellular matrix, vol I. CRC Press, Boca Raton, pp 143–178Google Scholar
  15. Furthmayr H, Mark Kvd (1982) The use of antibodies to connective tissue proteins in studies on their localization in tissues. In: Furthmayr H (ed) Immunochemistry of the extracellular matrix, vol II. CRC Press, Boca Raton, pp 90–117Google Scholar
  16. Gay S, Miller EJ (1978) Collagen in the physiology and pathology of connective tissue. Gustav Fischer, Stuttgart New YorkGoogle Scholar
  17. Gay S, Miller EJ (1983) What is collagen, what is not. Ultrastruct Pathol 4:365–377Google Scholar
  18. Gay S, Fietzek PP, Remberger K, Eder M, Kühn K (1975) Liver cirrhosis: immunofluorescence and biochemical studies demonstrate two types of collagen. Klin Wochenschr 53:205–208Google Scholar
  19. Giles CH (1975) Dye-fibre bonds and their investigation. In: Bird CI, Boston WS (eds) The theory of coloration of textiles. Dyers Company Publications Trust, Bradford, pp 41–110Google Scholar
  20. Grimand JA, Druguet M, Peyrol S, Chevalier O, Herbage D, Badrawy NE (1980) Collagen immunotyping in human liver: light and electron microscopic study. J Histochem Cytochem 28:1145–1156Google Scholar
  21. Grimmel HW (1953) Organic dyes. In: Gilman H (ed) Organic chemistry, and advanced treatise. John Wiley & Sons, New York, pp 243–373Google Scholar
  22. Grünstein N (1896) Über den Bau der grösseren menschlichen Arterien in verschiedenen Altersstufen. Arch Mikrosk Anat 47:583–654Google Scholar
  23. Gustavson KH (1956) Aldehyde tanning. The chemistry of tanning processes. Academic Press, New York, pp 244–282Google Scholar
  24. Gusterson BA, Warburton MJ, Mitchell D, Ellison M, Neville AM, Rudland PS (1982) Distribution of myoepithelial cells and basement membrane proteins in the normal breast and in benign and malignant breast diseases. Cancer Res 42:4763–4770Google Scholar
  25. Gusterson BA, Warburton MJ, Mitchell D, Kraft N, Hancock WW (1984) Invading squamous cell carcinoma can retain a basal lamina. An immunohistochemical study using a monoclonal antibody to type IV collagen. Lab Invest 51:82–87Google Scholar
  26. Haust MD (1983) Atherosclerotic lesions and sequelae. In: Silver MD (ed) Cardiovascular pathology. Churchill Livingstone, New York, pp 191–315Google Scholar
  27. Hinglais-Guillaud N, Galle P (1961) Presence of collagen fibers in intraluminal regions of human pathologic glomerulus. CR Acad Sci 253:1627–1629. Quoted from Collagen Currents 3 (No. 9):33 (March) 1963Google Scholar
  28. Hølund B, Clausen PP, Clemmensen I (1981) The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissues. Histochemistry 72:291–299Google Scholar
  29. Kaku T, Ekem JK, Lindayen C, Bailey DJ, van Nostrand AWP, Farber E (1983) Comparison of formalin- and acetone-fixation for immunohistochemical detection of carcinoembryonic antigen (CEA) and keratin. Am J Clin Pathol 80:806–815Google Scholar
  30. Kefalides NA (1966) A collagen of unusual composition and a glycoprotein isolated from canine glomerular basement membrane. Biochem Biophys Res Commun 22:26–32Google Scholar
  31. Kefalides NA, Winzler RJ (1966) The chemistry of glomerular basement membrane and its relation to collagen. Biochemistry 5:702–713Google Scholar
  32. Kent G, Gay S, Inouye T, Bahu R, Minick O, Popper H (1976) Vitamin A-containing lipocytes and formation of type III collagen in liver injury. Proc Natl Acad Sci USA 73:3719–3722Google Scholar
  33. Kölliker A (1863) Handbuch der Gewebelehre des Menschen, 4 Aufl. Engelmann, LeipzigGoogle Scholar
  34. Krahler SE (1955) Miscellaneous dyes. In: Lubs HA (ed) The chemistry of synthetic dyes and pigments. Reinhold, New York, pp 228–390Google Scholar
  35. Kühn K (1960) Über den Ursprung des Querstreifungsmusters bei Kollagen. Leder 11:110–117Google Scholar
  36. Lehrell F (1903) Histochemische Untersuchungen über das bindegewebige Gerüst der Milz der Wirbeltiere. Int Monatsschr Anat Physiol 20:171–206Google Scholar
  37. Leushner JRA, Haust MD (1986) Interstitial collagens in fibrous arteriosclerotic lesions of human aorta. Pathol Biol (Paris) 34:14–18Google Scholar
  38. Lillie RD (1951) The allochrome procedure: a differential method segregating the connective tissue: collagen, reticulum and basement membranes into two groups. Am J Clin Pathol 21:484–487Google Scholar
  39. Lillie RD (1952) Histochemistry of connective tissues: collagen, reticulum, basement membranes, sarcolemma, ocular membranes. Lab Invest 1:30–45Google Scholar
  40. Lillie RD (1969) Biological stains, 8th edn. Williams & Wilkins, BaltimoreGoogle Scholar
  41. Madri J, Dreyer B, Pitlick FA, Furthmayr H (1980) The collagenous components of the subendothelium. Correlation of structure and function. Lab Invest 43:303–315Google Scholar
  42. Mall F (1902) On the development of the connective tissues from the connective-tissue syncytium. Am J Anat 1:329–365Google Scholar
  43. Mark Kvd (1978) Biochemical processes in cartilage and bone formation. Effect of collagen on the chondrogenic differentiation of somite and limb bud mesenchyme. Fed Eur Biochem Soc Proc Meet 48:35–44Google Scholar
  44. Mark Kvd (1981) Localization of collagen types in tissues. Int Rev Connect Tissue Res 9:265–324Google Scholar
  45. Martinez-Hernandez A (1984) The hepatic extracellular matrix I. Electron immunohistochemical studies in normal rat liver. Lab Invest 51:57–74Google Scholar
  46. Maximow A (1927) Bindegewebe und blutbildende Gewebe. In: Mollendorff Wv (ed) Handbuch der mikroskopischen Anatomie des Menschen, 2 Bd, 1 Teil Springer, Berlin, pp 232–583Google Scholar
  47. McCullagh KG, Balian G (1975) Collagen characterization and cell transformation in human arteriosclerosis. Nature 258:73–75Google Scholar
  48. McCullagh KG, Duance VC, Bishop KA (1980) The distribution of collagen types I, III and V (AB) in normal and arteriosclerotic human aorta. J Pathol 130:45–55Google Scholar
  49. Miller EJ, Gay S (1982) Collagen, an overview. Methods Enzymol 82 Pt A: 3–32Google Scholar
  50. Miller EJ, Matukas VJ (1969) Chick cartilage collagen: a new type of alpha I chain not present in bone or skin of the species. Proc Natl Acad Sci USA 64:1264–1268Google Scholar
  51. Misra RP, Berman LB (1966) Chemistry of mammalian glomerular basement membrane. Clin Res 14:76Google Scholar
  52. Pratt LS (1948) The chemistry and physics of organic pigments. John Wiley & Sons, New YorkGoogle Scholar
  53. Puchtler H, Isler H (1958) The effect of phosphomolybdic acid on the stainability of connective tissues by various dyes. J Histochem Cytochem 6:265–270Google Scholar
  54. Puchtler H, Meloan SN (1985) On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions. Histochemistry 82:201–204Google Scholar
  55. Puchtler H, Sweat F (1963) Influence of various pretreatments on the staining properties of connective tissue fibers. Ann d'Histochim 8:suppl 1:189–198Google Scholar
  56. Puchtler H, Sweat F (1964) Histochemical specifity of staining methods for connective tissue fibers: resorcin-fuchsin and van Gieson's picro-fuchsin. Histochemie 4:24–34Google Scholar
  57. Purchtler H, Waldrop FS, Meloan SN, Terry MS, Conner HM (1970) Methacarn (methanol-Carnoy) fixation: practical and theoretical considerations. Histochemie 21:97–116Google Scholar
  58. Puchtler H, Meloan SN, Brewton BR (1974) On the binding of Schiff's reagent in the PAS reactions: effects of molecular configurations in models. Histochemistry 40:291–299Google Scholar
  59. Puchtler H, Meloan SN, Waldrop FS (1988) Are picro-dye reactions for collagens quantitative? Chemical and histochemical considerations.Histochemistry 88:243–256Google Scholar
  60. Ranvier L (1889) Traité technique d'histologie, 2nd edn. Librairie F. Savy, ParisGoogle Scholar
  61. Remberger K, Gay S, Fietzek PP (1975) Immunohistochemische Untersuchungen zur Kollagencharakterisierung in Lebercirrhosen. Virchows Arch 367:231–240Google Scholar
  62. Richards KM (1936) Phosphotungstic lakes. J Soc Dyers Colour 52:378–380Google Scholar
  63. Risse A (1853) Observations quaedam de artiarum statu normali atque pathologico. Dalkowski, RegiomontiGoogle Scholar
  64. Roll FJ, Madri JA, Albert T, Furthmayr H (1980) Codistribution of collagen types IV and AB2 in basement membranes and mesangium of the kidney. An immunoferritin study of ultrathin frozen sections. J Cell Biol 85:597–616Google Scholar
  65. Rowe FM (1938) The development of the chemistry of commercial synthetic dyes (1856–1938). The Institute of Chemistry of Great Britain and Ireland, LondonGoogle Scholar
  66. Schaeffer A (1963) Chemie der Farbstoffe und deren Anwendung. Theodor Steinkopff, Dresden LeipzigGoogle Scholar
  67. Schiefferdecker P, Kossel A (1891) Gewebelehre mit besonderer Berücksichtigung des menschlichen Körpers. Erst Abt. Harald Bruhn, BraunschweigGoogle Scholar
  68. Schupppan D, Becker J, Boehm H, Hahn EG (1986) Immunofluorescent localization of type V collagen as a fibrillar component of the interstitial connective tissue of oral mucosa, artery and liver. Cell Tissue Res 243:535–543Google Scholar
  69. Seidenfaden W (1957) Künstliche organische Farbstoffe und ihre Anwendungen. Ferdinand Enke, StuttgartGoogle Scholar
  70. Silbermann (1891) Die diffuse Skerose der Aorta, nebst Bemerkungen über das Traktionsancurysma der Kinder. Inaug-Diss, DorpatGoogle Scholar
  71. Sokoloff N (1888) Über die venöse Hyperämie der Milz. Virchows Arch 112:209–236Google Scholar
  72. Sternberger L (1979) Immunocytochemistry, 2nd edn. John Wiley & Sons, New YorkGoogle Scholar
  73. Sweat F, Puchtler H, Woo P (1964) Periodic acid-Schiff — picro Sirius Supra Blue GL: a light fast modification of Lillie's allochrome stain. Arch Pathol 78:73–75Google Scholar
  74. Sweat F, Puchtler H, Sesta JJ (1968) Periodic acid-Schiff — phosphomolybdic acid — Sirius Supra Blue FGL-CE: a convenient stain for demonstration of glomerular fibrosis. Arch Pathol 86:33–39Google Scholar
  75. Thoma R (1891) Über Gefäss- und Bindegewebsneubildung in der Arterienwand. Beitr Pathol Anat 10:433–448Google Scholar
  76. Trelstad RL (1974) Human aorta collagen: evidence for three distinct species. Biochem Biophys Res Commun 57:717–725Google Scholar
  77. Voss B, Rauterberg J, Allam S, Pott G (1980) Distribution of collagen type I and type III and of two collagenous components of basement membranes in the human liver. Pathol Res Pract 170:50–60Google Scholar
  78. Waldrop FS, Puchtler H (1981) PAS-PTA-Sirius Supra Blue FGL-CF stain for fibrosis in Carnoy-fixed tissues. Arch Pathol Lab Med 105:439Google Scholar
  79. Warburton MJ, Mitchell D, Ormerod EJ, Rudland P (1982) Distribution of myoepithelial cells and basement membrane proteins in the resting, pregnant, lactating and involuting rat mammary gland. J Histochem Cytochem 30:667–676Google Scholar
  80. Wick G, Olsen BR, Timpl R (1978) Immunohistologic analysis of fetal and dermatosparactic calf and sheep skin with antisera to procollagen and collagen type I. Lab Invest 39:151–156Google Scholar
  81. Zollinger H (1987) Color chemistry: syntheses, properties and applications of organic dyes and pigments. Verlag Chemie, WeinheimGoogle Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • R. N. Rao
    • 1
  • P. M. Fallman
    • 1
  • D. Greer Falls
    • 1
  • S. N. Meloan
    • 1
  1. 1.Department of PathologyMedical College of GeorgiaAugustaUSA

Personalised recommendations