, Volume 91, Issue 4, pp 283–289 | Cite as

A comparative study of PAS-phosphotungstic acid-Diamine Supra Blue FGL and immunological reactions for type I collagen

  • R. N. Rao
  • P. M. Fallman
  • D. Greer Falls
  • S. N. Meloan


Immunohistochemical techniques proved valuable in histological studies of various types of collagens. However drawbacks include non-specific reactions of antibodies, masking of antigens, and the high cost of antibodies. This study was undertaken to ascertain the specificity of the PAS-phosphotungstic acid-Diamine Supra Blue FGL (PAS PTA-DSB-FGL) reaction for type I collagen, differentiating it from other collagens. Duplicate series of methaearn-fixed sections of various tissues were treated with the PAS-PTA-DSB FGL reaction and the peroxidase-antiperoxidase (PAP) technique for type I collagen and the staining patterns were compared. Fibers binding the blue dye were found only at sites reacting with antibodies against type I collagen. These observations indicate that the PAS-PTA-DSB FGL procedure is suitable for visualization of type I collagen, e.g. in screening of large series of sections and in the practice of surgical and autopsy pathology.


Public Health Collagen Large Series Staining Pattern Histological Study 
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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • R. N. Rao
    • 1
  • P. M. Fallman
    • 1
  • D. Greer Falls
    • 1
  • S. N. Meloan
    • 1
  1. 1.Department of PathologyMedical College of GeorgiaAugustaUSA

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