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Alkaline lead citrate: A selective stain for glutaraldehyde precipitates of indolamines and primary catecholamines in electron microscopy

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Summary

Test-tube experiments proved that alkaline lead citrate (Reynolds, 1963), which is generally used as an electron-opaque stain, specifically reacts with precipitates formed by glutaraldehyde and biogenic amines (indolamines, primary catecholamines). Glutaraldehyde/osmium tetroxide fixation and staining of thin sections with alkaline lead citrate is recommended as an optimal preparation method of studying the above-mentioned amines at a fine structural level.

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References

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Authors and Affiliations

  1. Department of Anatomy, University of Cologne, Cologne, FRG

    Peter Böck & Karin Gorgas

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  1. Peter Böck
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  2. Karin Gorgas
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Supported by a grant from Deutsche Forschungsgemeinschaft, project No. Bo 525/1

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Böck, P., Gorgas, K. Alkaline lead citrate: A selective stain for glutaraldehyde precipitates of indolamines and primary catecholamines in electron microscopy. Histochemistry 47, 59–62 (1976). https://doi.org/10.1007/BF00492993

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  • DOI: https://doi.org/10.1007/BF00492993

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