Fresenius' Zeitschrift für analytische Chemie

, Volume 332, Issue 3, pp 258–260 | Cite as

Peroxidase-catalysed fluorescence reaction using tyrosine as substrate

  • Yun-Xiang Ci
  • Lie Chen
  • Shan Wei
Original Papers Biochemical Analysis

Summary

The fluorescence reaction using the catalytic effect of horse radish peroxidase on the oxidation of tyrosine by hydrogen peroxide has been investigated. The reaction product fluoresces at 412 nm (excitation at 316 nm). Microamounts of hydrogen peroxide and tyrosine can be determined with good accuracy and reproducibility. The proposed method has been used to determine glucose and tyrosine in human serum. The results are satisfactory. The characteristics of tyrosine have been compared with those of homovanillic acid in the enzyme-catalysed reaction system.

Keywords

Oxidation Hydrogen Glucose Physical Chemistry Analytical Chemistry 

Peroxidase-katalysierte Fluorescenzreaktion mit Tyrosin als Substrat

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References

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    Guilbault G-G, Brignac P-J Jr, Juneau M (1968) Anal Chem 40:1256Google Scholar
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    Guilbault G-G, Brignac P-J Jr, Zimmer M (1968) Anal Chem 40:190Google Scholar
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    Guilbault G-G (1968) Anal Biochem 26:1Google Scholar
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    Briggs G-E, Haldane J-B-S (1925) Biochem J 19:338Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Yun-Xiang Ci
    • 1
  • Lie Chen
    • 1
  • Shan Wei
    • 1
  1. 1.Department of ChemistryPeking UniversityBeijingPeople's Republic of China

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