Peroxidase-catalysed fluorescence reaction using tyrosine as substrate
The fluorescence reaction using the catalytic effect of horse radish peroxidase on the oxidation of tyrosine by hydrogen peroxide has been investigated. The reaction product fluoresces at 412 nm (excitation at 316 nm). Microamounts of hydrogen peroxide and tyrosine can be determined with good accuracy and reproducibility. The proposed method has been used to determine glucose and tyrosine in human serum. The results are satisfactory. The characteristics of tyrosine have been compared with those of homovanillic acid in the enzyme-catalysed reaction system.
KeywordsOxidation Hydrogen Glucose Physical Chemistry Analytical Chemistry
Peroxidase-katalysierte Fluorescenzreaktion mit Tyrosin als Substrat
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